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European Journal of Nutrition

, Volume 52, Issue 3, pp 1233–1242 | Cite as

Reduced cholesterol levels in renal membranes of undernourished rats may account for urinary Na+ loss

  • Fabiana S. T. Oliveira
  • Leucio D. Vieira-Filho
  • Edjair V. Cabral
  • Luzia S. Sampaio
  • Paulo A. Silva
  • Vera C. O. Carvalho
  • Adalberto Vieyra
  • Marcelo Einicker-Lamas
  • Vera L. M. Lima
  • Ana D. O. Paixão
Original Contribution

Abstract

Purpose

It has been demonstrated that reabsorption of Na+ in the thick ascending limb is reduced and the ability to concentrate urine can be compromised in undernourished individuals. Alterations in phospholipid and cholesterol content in renal membranes, leading to Na+ loss and the inability to concentrate urine, were investigated in undernourished rats.

Methods

Sixty-day-old male Wistar rats were utilized to evaluate (1) phospholipid and cholesterol content in the membrane fraction of whole kidneys, (2) cholesterol content and the levels of active Na+ transporters, (Na+ + K+)ATPase and Na+-ATPase, in basolateral membranes of kidney proximal tubules, and (3) functional indicators of medullary urine concentration.

Results

Body weight in the undernourished group was 73 % lower than in control. Undernourishment did not affect the levels of cholesterol in serum or in renal homogenates. However, membranes of whole kidneys revealed 56 and 66 % reduction in the levels of total phospholipids and cholesterol, respectively. Furthermore, cholesterol and (Na+ + K+)ATPase activity in proximal tubule membranes were reduced by 55 and 68 %, respectively. Oxidative stress remained unaltered in the kidneys of undernourished rats. In contrast, Na+-ATPase activity, an enzyme with all regulatory components in membrane, was increased in the proximal tubules of undernourished rats. Free water clearance and fractional Na+ excretion were increased by 86 and 24 %, respectively, and urinary osmolal concentration was 21 % lower in undernourished rats than controls.

Conclusion

Life-long undernutrition reduces the levels of total phospholipids and cholesterol in membranes of renal tubular cells. This alteration in membrane integrity could diminish (Na+ + K+)ATPase activity resulting in reduced Na+ reabsorption and urinary concentrating ability.

Keywords

Undernutrition Phospholipids Kidney (Na+ + K+)ATPase Na+-ATPase Free water clearance 

Notes

Acknowledgments

The authors thank Glória Costa-Sarmento for technical support and BioMedES for editing the manuscript. This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/PROCAD 519/2010), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco, Brazilian Research Council (470740/2010-8) and Fundação Carlos Chagas Filho de Amparo à pesquisa do Estado do Rio de Janeiro, Brazil.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Fabiana S. T. Oliveira
    • 1
    • 2
  • Leucio D. Vieira-Filho
    • 1
    • 3
  • Edjair V. Cabral
    • 1
    • 3
  • Luzia S. Sampaio
    • 3
    • 4
  • Paulo A. Silva
    • 3
    • 4
  • Vera C. O. Carvalho
    • 2
  • Adalberto Vieyra
    • 3
    • 4
  • Marcelo Einicker-Lamas
    • 3
    • 4
  • Vera L. M. Lima
    • 2
  • Ana D. O. Paixão
    • 1
    • 3
  1. 1.Departamento de Fisiologia e Farmacologia, Centro de Ciências BiológicasUniversidade Federal de PernambucoRecifeBrazil
  2. 2.Department of BiochemistryFederal University of PernambucoRecifeBrazil
  3. 3.National Institute of Science and Technology in Structural Biology and BioimageRio de JaneiroBrazil
  4. 4.Carlos Chagas Filho Institute of BiophysicsFederal University of Rio de JaneiroRio de JaneiroBrazil

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