European Journal of Nutrition

, Volume 52, Issue 2, pp 457–466 | Cite as

Active hexose-correlated compound and Bifidobacterium longum BB536 exert symbiotic effects in experimental colitis

  • Borja Ocón
  • Andrea Anzola
  • Mercedes Ortega-González
  • Antonio Zarzuelo
  • María D. Suárez
  • Fermín Sánchez de Medina
  • Olga Martínez-AugustinEmail author
Original Contribution



Active hexose-correlated compound (AHCC) is a commercial extract obtained from Basidiomycetes under controlled conditions, yielding a 74 % content in oligosaccharides, especially α-glucans. AHCC has a number of therapeutic effects, including intestinal anti-inflammatory activity. Bifidobacterium longum BB536 is a probiotic with potential health-promoting effect at the gut level. The purpose of the present study was to evaluate the possibility of synergism between AHCC, which is believed to act as a prebiotic, and B. longum BB536.


We used the trinitrobenzene sulfonic acid model (TNBS) of colitis in rats. AHCC (100 or 500 mg kg−1) and B. longum BB536 (5 × 106 CFU rat−1 day−1) were administered together or separately for 7 days prior to colitis induction and then for another 7 days and compared with control (noncolitic) and TNBS rats.


The results show that both treatments had intestinal anti-inflammatory activity separately, which was enhanced when used in combination, as shown by changes in body weight gain, colonic weight to length ratio, myeloperoxydase activity and iNOS expression. Interestingly, the association of AHCC 100 mg kg−1 + B. longum BB536 showed the highest anti-inflammatory activity.


Our data provide a preclinical experimental basis for the synergistic effect of AHCC and B. longum BB536 on inflammatory bowel disease.


Active hexose-correlated compound Bifidobacterium longum BB536 Symbiotic Colitis 



This study was supported by funds from the Spanish Ministery of Science and Innovation (SAF2008-01432, AGL2008-04332, SAF2011-22922, SAF2011-22812), by funds from Junta de Andalucía (CTS-6736), by a GREIB (Granada Research of Excellence Initiative on BioHealth) translational grant and by the Fundación Ramón Areces. BO and MOG are supported by a fellowship of the Ministery of Education and Science of Spain. CIBERehd (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) is funded by the Instituto de Salud Carlos III.

Conflict of interest

AminoUp Chemical (Sapporo, Japan) partially supported these experiments.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Borja Ocón
    • 1
  • Andrea Anzola
    • 1
  • Mercedes Ortega-González
    • 2
  • Antonio Zarzuelo
    • 1
  • María D. Suárez
    • 2
  • Fermín Sánchez de Medina
    • 1
  • Olga Martínez-Augustin
    • 2
    Email author
  1. 1.Department of Pharmacology, CIBERehd, School of PharmacyUniversity of GranadaGranadaSpain
  2. 2.Department of Biochemistry and Molecular Biology II, CIBERehd, School of PharmacyUniversity of GranadaGranadaSpain

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