Simultaneous ingestion of dietary proteins reduces the bioavailability of galloylated catechins from green tea in humans
- 621 Downloads
To investigate the influence of dietary proteins (casein, soy protein) and skimmed milk on the plasma kinetics of green tea (GT) catechins.
In a randomized cross-over design with one-week intervals, 24 healthy normal-weight women consumed a test drink containing 1.75 g GT extract with or without the addition of different proteins. Treatments were GT (control), GT with skimmed milk (GT + M), GT with caseinate (GT + CS), or GT with soy protein (GT + S). Venous blood samples were taken before and several times during a period of 4.5 h after consumption of the test drink. Plasma concentrations of catechins were analyzed by HPLC with electrochemical detection.
Compared to control, consumption of GT with milk, caseinate, or soy protein significantly reduced the bioavailability (mean area under the plasma concentration–time curve) of total catechins (means ± SEM; GT + M, 87 ± 5%; GT + CS, 79 ± 5%; GT + S, 88 ± 4%), epigallocatechin gallate (GT + M, 68 ± 4%; GT + CS, 63 ± 5%; GT + S, 76 ± 5%), and epicatechin gallate (GT + M, 68 ± 5%; GT + CS, 66 ± 6%; GT + S, 77 ± 6%), while the bioavailability of non-galloylated catechins such as epigallocatechin (GT + M, 134 ± 9%; GT + CS, 118 ± 9 %; GT + S, 123 ± 8%) and epicatechin (GT + M, 125 ± 10%; GT + CS, 114 ± 11%; GT + S, 110 ± 8%) significantly increased. No significant differences in bioavailability of GT catechins were observed between the treatments GT + M, GT + CS, or GT + S.
Simultaneous ingestion of dietary proteins reduces the bioavailability of galloylated catechins from GT in humans.
KeywordsCatechins Flavan-3-ols Bioavailability Dietary protein Human study
We are indebted to Plantextrakt (Vestenbergsgreuth, Germany) for providing the green tea extract and analyses, to Meggle (Wasserburg, Germany) for providing the caseinate, to the Solae Company (St. Louis, North America) for providing the soy protein, to Maike Jürgensen for valuable technical assistance, and to Isabella Serafin for performing the venipunctures.
Conflict of interest
- 2.Rietveld A, Wiseman S (2003) Antioxidant effects of tea: evidence from human clinical trials. J Nutr 133:3285S–3292SGoogle Scholar
- 7.Arts IC, Hollman PC, Feskens EJ, Bueno de Mesquita HB, Kromhout D (2001) Catechin intake might explain the inverse relation between tea consumption and ischemic heart disease: the Zutphen Elderly Study. Am J Clin Nutr 74:227–232Google Scholar
- 8.Hertog MG, Sweetnam PM, Fehily AM, Elwood PC, Kromhout D (1997) Antioxidant flavonols and ischemic heart disease in a Welsh population of men: the Caerphilly Study. Am J Clin Nutr 65:1489–1494Google Scholar
- 9.Serafini M, Ghiselli A, Ferro-Luzzi A (1996) In vivo antioxidant effect of green and black tea in man. Eur J Clin Nutr 50:28–32Google Scholar
- 19.Lee MJ, Wang ZY, Li H, Chen L, Sun Y, Gobbo S, Balentine DA, Yang CS (1995) Analysis of plasma and urinary tea polyphenols in human subjects. Cancer Epidemiol Biomarkers Prev 4:393–399Google Scholar
- 23.Williamson MP (1994) The structure and function of proline-rich regions in proteins. Biochem J 297(Pt 2):249–260Google Scholar