Probiotics and dietary counselling targeting maternal dietary fat intake modifies breast milk fatty acids and cytokines
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Breast milk fatty acids possess immunomodulatory properties, and new intervention strategies beyond supplementation of maternal diet with single oils are called for. The objective of the present study was to evaluate the effect of dietary intervention during pregnancy and breastfeeding on breast milk fatty acid and cytokine composition.
Pregnant women were randomised into three study groups: dietary intervention with probiotics (diet/probiotic) or with placebo (diet/placebo) and a control group (control/placebo). Dietary intervention included dietary counselling and provision of rapeseed oil-based food products. The probiotics used were Lactobacillus rhamnosus GG and Bifidobacterium lactis Bb12 in combination. Dietary intake was evaluated by food records at every trimester of pregnancy and 1 month postpartum. Breast milk samples were collected after birth (colostrum) and 1 month after delivery for fatty acid and cytokine analysis (n = 125).
Dietary intervention improved the quality of fat in the diet. In breast milk, the proportion of α-linolenic acid and total n-3 fatty acids was higher in both dietary intervention groups compared with control group (p < 0.05). In the diet/probiotic group, the γ-linolenic acid content was higher compared with the diet/placebo group (p < 0.05). The concentrations of TNF-α, IL-10, IL-4 and IL-2 were higher in both dietary intervention groups compared with controls, and furthermore, long-chain n-3 fatty acids were associated with several cytokines in colostrum samples.
The present intervention demonstrated the possibility of modifying breast milk immunomodulatory factors by dietary means.
KeywordsBreast milk Fatty acid Cytokine Dietary counselling
The present study was supported by the Social Insurance Institution of Finland, the Juho Vainio Foundation and the Sigrid Juselius Foundation. Food products from Raisio plc (Raisio, Finland), B. lactis from Chr. Hansen (Hoersholm, Denmark) and L. rhamnosus GG from Valio Ltd (Helsinki, Finland).
Conflict of interest
Päivi Laakso was employed by Raisio plc, other authors have no conflict of interest.
- 7.Field CJ (2005) The immunological components of human milk and their effect on immune development in infants. J Nutr 135:1–4Google Scholar
- 9.Prescott SL, Wickens K, Westcott L et al (2008) Supplementation with Lactobacillus rhamnosus or Bifidobacterium lactis probiotics in pregnancy increases cord blood interferon-γ and breast milk transforming growth factor-β and immunoglobulin A detection. Clin Exp Allergy 38:1606–1614CrossRefGoogle Scholar
- 13.Lauritzen L, Halkjaer LB, Mikkelsen TB, Olsen SF, Michaelsen KF, Loland L, Bisgaard H (2006) Fatty acid composition of human milk in atopic Danish mothers. Am J Clin Nutr 84:190–196Google Scholar
- 19.Fineli–Finnish Food Composition Database (2010) National Institute for Health and Welfare. http://www.fineli.fi Accessed Nov 2010
- 21.Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509Google Scholar
- 22.Christie WW (2003) Lipid analysis–isolation, separation, identification and structural analysis of lipids, 3rd edn. The Oily Press, BridgewaterGoogle Scholar
- 24.Van Goor SA, Dijick-Brouwer DA, Hadders-Algra M, Doornbos B, Erwich JJ, Schaafsma A, Muskiet FA (2009) Human milk arachidonic acid and docosahexaenoic acid contents increase following supplementation during pregnancy and lactation. Prostaglandins Leukot Essent Fatty Acids 80:65–69CrossRefGoogle Scholar
- 25.Francois CA, Connor SL, Bolewicz LC, Connor WE (2003) Supplementing lactating women with flaxseed oil does not increase docosahexaenoic acid in their milk. Am J Clin Nutr 77:226–233Google Scholar
- 37.Hawkes JS, Bryan DL, Makrides M, Neuman MA, Gibson RA (2002) A randomized trial of supplementation with docosahexaenoic acid-rich tuna oil and its effects on the human milk cytokines interleukin 1β, interleukin 6, and the tumor necrosis factor α. Am J Clin Nutr 75:754–760Google Scholar
- 41.Koletzko B, Cetin I, Brenna JT for the Perinatal Lipid Intake Working Group (2007) Dietary fat intakes for pregnant and lactating women. Consensus Statement. Br J Nutr 98:873–877Google Scholar