European Journal of Nutrition

, Volume 50, Issue 4, pp 271–277 | Cite as

Effects of a diet with inulin-enriched pasta on gut peptides and gastric emptying rates in healthy young volunteers

  • Francesco RussoEmail author
  • Caterina Clemente
  • Michele Linsalata
  • Marisa Chiloiro
  • Antonella Orlando
  • Emanuele Marconi
  • Guglielmina Chimienti
  • Giuseppe Riezzo
Original Contribution



Our group has previously shown that the administration of pasta enriched along with the prebiotic inulin induces a significant reduction in triglyceride and glucose levels with a significant delay in gastric emptying (GE) rates. This protective effect may occur by affecting the release of a number of gut peptides involved in the control of gastrointestinal motility. The aim of the present study was to evaluate the effects of inulin-enriched pasta on the circulating levels of neurotensin (NT), somatostatin (SS), and corticotropin-releasing factor (CRF) in relation to the GE time in young healthy subjects.


Twenty healthy young male volunteers completed a randomized double-blind crossover study consisting of a 2-week run-in period and two 5-week study periods (11% inulin-enriched/control pasta), with an 8-week wash-out period in between. Gut peptide concentrations were evaluated by radioimmunoassay. GE time was evaluated by ultrasonography.


The prebiotic treatment significantly increased the area under the curve (AUC) values of both NT and SS (p < 0.05 Dunn’s post-test). With regard to gastric motility, along with a significant delay in both the final time and T 1/2 gastric emptying time, a positive correlation was found between T 1/2 and SS AUC values (r = 0.57, p = 0.009) in the inulin-enriched pasta group.


These results support the hypothesis that inulin plays an active role in mechanisms affecting the release of these gut peptides, which may modulate the gastric emptying of digesta.


Corticotropin-releasing factor Diet Fibre Gastric emptying Gut peptides Inulin Metabolic syndrome Neurotensin Somatostatin 



This work was supported by a grant from the Italian Ministry of Health (#132).


  1. 1.
    Mancia G, Bombelli M, Facchetti R et al (2008) Long-term risk of diabetes, hypertension and left ventricular hypertrophy associated with the metabolic syndrome in a general population. J Hypertens 26:1602–1611CrossRefGoogle Scholar
  2. 2.
    Kaur N, Gupta AK (2002) Applications of inulin and oligofructose in health and nutrition. J Biosci 27:703–714CrossRefGoogle Scholar
  3. 3.
    Spiller GA (2001) Definitions of dietary fibres. In: Spiller GA (ed) CRC handbook of dietary fibres in human nutrition, 3rd edn. CRC press, Boca Raton, p 709CrossRefGoogle Scholar
  4. 4.
    Schulze MB, Liu S, Rimm EB, Manson JE, Willett WC, Hu FB (2004) Glycemic index, glycemic load, and dietary fiber intake and incidence of type 2 diabetes in younger and middle-aged women. Am J Clin Nutr 80:348–356Google Scholar
  5. 5.
    Cuche G, Cuber JC, Malbert CH (2000) Ileal short-chain fatty acids inhibit gastric motility by a humoral pathway. Am J Physiol (Gastrointest Liver Physiol) 279:G925–G930Google Scholar
  6. 6.
    Cherbut C (2002) Inulin and oligo-fructose in the dietary fibre concept. Br J Nutr 87:S159–S162CrossRefGoogle Scholar
  7. 7.
    Cherbut C (2003) Motor effects of short-chain fatty acids and lactate in the gastrointestinal tract. Proc Nutr Soc 62:95–99CrossRefGoogle Scholar
  8. 8.
    Brighenti F (2007) Dietary fructans and serum triacylglycerols: a meta-analysis of randomized controlled trials. J Nutr 137(Suppl 11):S2552–S2556Google Scholar
  9. 9.
    Cali AM, Caprio S (2008) Obesity in children and adolescents. J Clin Endocrinol Metab 93(Suppl 1):S31–S36CrossRefGoogle Scholar
  10. 10.
    Miller M, Seidler A, Moalemi A, Pearson TA (1998) Normal triglyceride levels and coronary artery disease events: the Baltimore coronary observational long-term study. J Am Coll Cardiol 31:1252–1257CrossRefGoogle Scholar
  11. 11.
    Russo F, Chimienti G, Riezzo G et al (2008) Inulin-Enriched pasta affects lipid profile and Lp (a) concentrations in Italian young healthy male volunteers. Eur J Nutr 47:453–459CrossRefGoogle Scholar
  12. 12.
    Russo F, Riezzo G, Chiloiro M et al (2010) Metabolic effects of a diet with inulin-enriched pasta in healthy young volunteers. Curr Pharm Des 16:825–831CrossRefGoogle Scholar
  13. 13.
    Naslund E, Gryback P, Backman L et al (1998) Distal small bowel hormones: correlation with fasting antroduodenal motility and gastric emptying. Dig Dis Sci 43:945–952CrossRefGoogle Scholar
  14. 14.
    Evers BM (2006) Neurotensin and growth of normal and neoplastic tissues. Peptides 27:2424–2433CrossRefGoogle Scholar
  15. 15.
    Di Lorenzo C, Lucanto C, Flores AF, Idries S, Hyman PE (1998) Effect of octreotide on gastrointestinal motility in children with functional gastrointestinal symptoms. J Pediatr Gastroenterol Nutr 27:508–509CrossRefGoogle Scholar
  16. 16.
    Martínez V, Wang L, Rivier JE, Vale W, Taché Y (2002) Differential actions of peripheral corticotropin-releasing factor (CRF), urocortin II, and urocortin III on gastric emptying and colonic transit in mice: role of CRF receptor subtypes 1 and 2. J Pharmacol Exp Ther 301:611–617CrossRefGoogle Scholar
  17. 17.
    Fukudo S, Nomura T, Hongo M (1998) Impact of corticotropin-releasing hormone on gastrointestinal motility and adrenocorticotropic hormone in normal controls and patients with irritable bowel syndrome. Gut 42:845–849CrossRefGoogle Scholar
  18. 18.
    Richard D, Lin Q, Timofeeva E (2002) The corticotropin-releasing factor family of peptides and CRF receptors: their roles in the regulation of energy balance. Eur J Pharmacol 40:189–197CrossRefGoogle Scholar
  19. 19.
    Taché Y, Kiank C, Stengel A (2009) A role for Corticotropin-releasing factor in functional gastrointestinal disorders. Current Gastroenterology Reports 11:270–277CrossRefGoogle Scholar
  20. 20.
    Marconi E, Carcea M (2001) Pasta from non-nutritional raw materials. Cereal Foods Word 46:522–530Google Scholar
  21. 21.
    Van Loo J, Coussement P, De Leenheer L, Hoebregs H, Smits G (1995) On the presence of inulin and oligo-fructose as natural ingredients in the Western diet. Crit. Rev. Food Sci Nutr 35:525–552CrossRefGoogle Scholar
  22. 22.
    Ku Y, Jansen O, Oles CJ, Lazar EZ, Rader JI (2003) Precipitation of inulin and oligo-glucoses by ethanol and other solvents. Food Chem 81:125–132CrossRefGoogle Scholar
  23. 23.
    American Association of Cereal Chemists Approved methods of the AACC, 11th edn. AACC, St Paul 2001Google Scholar
  24. 24.
    Roberfroid MB (1999) Caloric value of inulin and oligo-fructose. J Nutr 129:1436S–1437SGoogle Scholar
  25. 25.
    Bolondi L, Bortolotti M, Santi V, Calletti T, Gaiani S, Labò G (1985) Measurement of gastric emptying time by real time ultrasonography. Gastroenterology 89:752–759Google Scholar
  26. 26.
    Kivimäki M, Smith GD, Timpson NJ, Lawlor DA, Batty GD, Kähönen M et al (2008) Lifetime body mass index and later atherosclerosis risk in young adults: examining causal links using Mendelian randomization in the cardiovascular risk in young Finns study. Eur Heart J 29:2552–2560CrossRefGoogle Scholar
  27. 27.
    Zorio E, Falco C, Arnau MA, España F, Osa A, Ramon LA et al (2006) Lipoprotein (a) in young individuals as a marker of the presence of ischemic heart disease and the severity of coronary lesions. Haematologica 91:562–566Google Scholar
  28. 28.
    Li W, Shi YH, Yang RL, Cui J, Xiao Y, Wang B, Le GW (2010) Effect of somatostatin analog on high-fat diet-induced metabolic syndrome: involvement of reactive oxygen species. Peptides 31:625–629CrossRefGoogle Scholar
  29. 29.
    Taché Y, Perdue MH (2004) Role of peripheral CRF signalling pathways in stress-related alterations of gut motility and mucosal function. Neurogastroenterol Motil 16:137–142CrossRefGoogle Scholar
  30. 30.
    Williams CM, Jackson KG (2002) Inulin and oligo-fructose: effects on lipid metabolism from human studies. Br J Nutr 87(Suppl 2):S261–S264CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Francesco Russo
    • 1
    Email author
  • Caterina Clemente
    • 1
  • Michele Linsalata
    • 1
  • Marisa Chiloiro
    • 2
  • Antonella Orlando
    • 1
  • Emanuele Marconi
    • 3
  • Guglielmina Chimienti
    • 4
  • Giuseppe Riezzo
    • 2
  1. 1.Laboratory of Experimental BiochemistryI.R.C.C.S. “Saverio de Bellis”Castellana Grotte (Bari)Italy
  2. 2.Laboratory of Experimental PathophysiologyI.R.C.C.S. “Saverio de Bellis”Castellana Grotte (Bari)Italy
  3. 3.DiSTAAMUniversity of MoliseCampobassoItaly
  4. 4.Department of Biochemistry and Molecular BiologyUniversity of BariBariItaly

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