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European Journal of Nutrition

, Volume 50, Issue 2, pp 127–133 | Cite as

Antiobesity effect of polyphenolic compounds from molokheiya (Corchorus olitorius L.) leaves in LDL receptor-deficient mice

  • Li Wang
  • Masayuki Yamasaki
  • Takuya Katsube
  • Xufeng Sun
  • Yukikazu Yamasaki
  • Kuninori Shiwaku
Original Contribution

Abstract

Background

Dietary supplementation with polyphenolic compounds is associated with reduced diet-induced obesity and metabolic disorders in humans. The antioxidative properties of polyphenolic compounds contribute to their antiobesity effect in animal experiments and human studies.

Aim

The aim of the study was to investigate the antiobesity effect of polyphenolic compounds from molokheiya leaves in LDLR-/- mice fed high-fat diet and to elucidate the mechanism of this effect.

Methods

Three groups of LDLR-/- mice were fed with a high-fat diet, supplemented with 0% (control), 1 or 3% molokheiya leaf powder (MLP). Gene expression in the liver associated with lipid and glucose metabolism was analyzed, and physical parameters and blood biochemistry were determined.

Results

Compared to controls, mice body weight gain (P = 0.003), liver weight (P = 0.001) and liver triglyceride levels (P = 0.005) were significantly lower in the two MLP groups. Epididymal adipose tissue weight (P = 0.003) was reduced in the 3% MLP group. Liver tissue gene expression of gp91phox (NOX2), involved in oxidative stress, was significantly down-regulated (P = 0.005), and PPARα and CPT1A, related to the activation of β-oxidation, were significantly up-regulated (P = 0.025 and 0.006, respectively) in the 3% MLP group compared to the control group.

Conclusions

Our results demonstrate an antiobesity effect of polyphenolic compounds from molokheiya leaves and that this effect is associated with reduction in oxidative stress and enhancement of β-oxidation in the liver. Consumption of molokheiya leaves may be beneficial for preventing diet-induced obesity.

Keywords

Corchorus olitorius L. Molokheiya Obesity Oxidative stress β-oxidation Polyphenolic compounds 

Abbreviations

ACOX1

Acyl-coenzyme A oxidase 1

CPT1A

Carnitine palmitoyl transferase 1A

Ehhadh

Enoyl-coenzyme A hydratase/3-hydroxyacyl coenzyme A dehydrogenase

FAS

Fatty acid synthase

GK

Glucokinase

LDLR-/-

Low-density lipoprotein receptor-deficient

MLP

Molokheiya leaf powder

PPARα

Peroxisome proliferator activated receptor alpha

Notes

Acknowledgments

This study was supported in part by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology to M. Yamasaki and Grants for Scientific Research from Shimane Prefecture and collaborated with Shimane Institute for Industrial Technology and Izumoya Co. Ltd. We thank Dr. Jeff Burgess for checking our manuscript.

References

  1. 1.
    Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M, Shimomura I (2004) Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 114:1752–1761Google Scholar
  2. 2.
    Wei Y, Clark SE, Thyfault JP, Uptergrove GM, Li W, Whaley-Connell AT, Ferrario CM, Sowers JR, Ibdah JA (2009) Oxidative stress-mediated mitochondrial dysfunction contributes to angiotensin II-induced nonalcoholic fatty liver disease in transgenic Ren2 rats. Am J Pathol 174:1329–1337CrossRefGoogle Scholar
  3. 3.
    Knekt P, Kumpulainen J, Jarvinen R, Rissanen H, Heliovaara M, Reunanen A, Hakulinen T, Aromma A (2002) Flavonoid intake and risk of chronic diseases. Am J Clin Nutr 76:560–568Google Scholar
  4. 4.
    Bose M, Lambert JD, Ju J, Reuhl KR, Shapses SA, Yang CS (2008) The major green tea polyphenol, (-)-epigallocatechin-3-gallate, inhibits obesity, metabolic syndrome, and fatty liver disease in high-fat-fed mice. J Nutr 138:1677–1683Google Scholar
  5. 5.
    Katsube T, Imawaka N, Kawano Y, Yamazaki Y, Shiwaku K, Yamane Y (2006) Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem 97:25–31CrossRefGoogle Scholar
  6. 6.
    Enkhmaa B, Shiwaku K, Katsube T, Kitajima K, Anuurad E, Yamasaki M, Yamane Y (2005) Mulberry (Morus alba L.) leaves and their major flavonol quercetin 3-(6-malonylglucoside) attenuate atherosclerotic lesion development in LDL receptor-deficient mice. J Nutr 135:729–734Google Scholar
  7. 7.
    Dulloo AG, Duret C, Rohrer D, Girardier L, Mensi N, Fathi M, Chantre P, Vandermander J (1999) Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans. Am J Clin Nutr 70:1040–1045Google Scholar
  8. 8.
    Hsu CL, Wu CH, Huang SL, Yen GC (2009) Phenolic compounds rutin and o-coumaric acid ameliorate obesity induced by high-fat diet in rats. J Agric Food Chem 57:425–461CrossRefGoogle Scholar
  9. 9.
    Murase T, Nagasawa A, Suzuki J, Hase T, Tokimitsu I (2002) Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver. Int J Obes Relat Metab Disord 26:1459–1464CrossRefGoogle Scholar
  10. 10.
    Kuda T, Iwai A, Yano T (2004) Effect of red pepper Capsicum annuum var. conoides and garlic Allium sativum on plasma lipid levels and cecal microflora in mice fed beef tallow. Food Chem Toxicol 42:1695–1700CrossRefGoogle Scholar
  11. 11.
    Hsu CL, Yen GC (2007) Effect of gallic acid on high fat diet-induced dyslipidemia, hepatosteatosis, and oxidative stress in rats. Br J Nutr 98:727–735CrossRefGoogle Scholar
  12. 12.
    Honda S, Aoki F, Tanaka H, Kishida H, Nishiyama T, Okada S, Matsumoto I, Abe K, Mae T (2006) Effects of ingested turmeric oleoresin on glucose and lipid metabolisms in obese diabetic mice: a DNA microarray study. J Agric Food Chem 54:9055–9062CrossRefGoogle Scholar
  13. 13.
    Davalos A, de la Pena G, Sanchez-Martin CC, Guerra MT, Bartolome B, Lasuncion MA (2009) Effects of red grape juice polyphenols in NAPDH oxidase subunit expression in human neutrophils and mononuclear blood cells. Br J Nutr 102:1125–1135CrossRefGoogle Scholar
  14. 14.
    Feillet-Coudray C, Sutra T, Fouret G, Ramos J, Wrutniak-Cabello C, Cabello G, Cristol JP, Coudray C (2009) Oxidative stress in rats fed a high-fat high-sucrose diet and preventive effect of polyphenols: involvement of mitochondrial and NAD (P) H oxidase systems. Free Radic Biol Med 46:624–632CrossRefGoogle Scholar
  15. 15.
    Hashimoto T, Fujita T, Usuda N, Cook W, Qi C, Peters JM, Gonzalez FJ, Yeldandi AV, Rao MS, Reddy JK (1999) Peroxisomal and mitochondrial fatty acid beta-oxidation in mice nullizygous for both peroxisome proliferator-activated receptor alpha and peroxisomal fatty acyl-CoA oxidase. Genotype correlation with fatty liver phenotype. J Biol Chem 274:19228–19236CrossRefGoogle Scholar
  16. 16.
    Shimoda H, Tanaka J, Kikuchi M, Fukuda T, Ito H, Hatano T, Yoshida T (2009) Effect of polyphenol-rich extract from walnut on diet-induced hypertriglyceridemia in mice via enhancement of fatty acid oxidation in the liver. J Agric Food Chem 57:1786–1792CrossRefGoogle Scholar
  17. 17.
    Azuma K, Nakayama M, Koshioka M, Ippoushi K, Yamaguchi Y, Kohata K, Yamauchi Y, Ito H, Higashio H (1999) Phenolic antioxidants from the leaves of Corchorus olitorius L. J Agric Food Chem 47:3963–3966CrossRefGoogle Scholar
  18. 18.
    Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917Google Scholar
  19. 19.
    Kao YH, Hiipakka RA, Liao S (2000) Modulation of endocrine systems and food intake by green tea epigallocatechin gallate. Endocrinology 141:980–987CrossRefGoogle Scholar
  20. 20.
    Han LK, Sumiyoshi M, Zhang J, Liu MX, Zhang XF, Zheng YN, Okuda H, Kimura Y (2003) Anti-obesity action of Salix matsudana leaves (Part 1). Anti-obesity action by polyphenols of Salix matsudana in high fat-diet treated rodent animals. Phytother Res 17(10):1188–1194CrossRefGoogle Scholar
  21. 21.
    Nemali MR, Usuda N, Reddy MK, Oyasu K, Hashimoto T, Osumi T, Rao MS, Reddy JK (1988) Comparison of constitutive and inducible levels of expression of peroxisomal beta-oxidation and catalase genes in liver and extrahepatic tissue of rat. Cancer Res 48:5316–5324Google Scholar
  22. 22.
    Aoyama T, Peters JM, Iritani N, Nakajima T, Furihata K, Hashimoto T, Gonzalez FJ (1998) Altered constitutive expression of fatty acid-metabolizing enzymes in mice lacking the peroxisome proliferator-activated receptor α (PPARα). J Biol Chem 273:5678–5684CrossRefGoogle Scholar
  23. 23.
    Schoonjans K, Staels B, Auwerx J (1996) Role of peroxisome proliferator-activated receptor (PPAR) in mediating the effects of fibrates and fatty acids on gene expression. J Lipid Res 37:907–925Google Scholar
  24. 24.
    Latruffe N, Vamecq J (1997) Peroxisome proliferators and peroxisome proliferator activated receptors (PPARs) as regulators of lipid metabolism. Biochimie 79:81–94CrossRefGoogle Scholar
  25. 25.
    Roberts CK, Barnard RJ, Sindhu RK, Jurczak M, Ehdaie A, Vaziri ND (2006) Oxidative stress and dysregulation of NAD (P) H oxidase and antioxidant enzymes in diet-induced metabolic syndrome. Metabolism 55:928–934CrossRefGoogle Scholar
  26. 26.
    Valentova K, Truong NT, Moncion A, de Waziers I, Ulrichova J (2007) Induction of glucokinase mRNA by dietary phenolic compounds in rat liver cells in vitro. J Agric Food Chem 55:7726–7731CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Li Wang
    • 1
  • Masayuki Yamasaki
    • 1
  • Takuya Katsube
    • 2
  • Xufeng Sun
    • 1
  • Yukikazu Yamasaki
    • 2
  • Kuninori Shiwaku
    • 1
  1. 1.Department of Environmental and Preventive MedicineShimane University School of MedicineIzumo City, ShimaneJapan
  2. 2.Shimane Institute for Industrial TechnologyMatsue City, ShimaneJapan

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