Lupin protein compared to casein lowers the LDL cholesterol:HDL cholesterol-ratio of hypercholesterolemic adults
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Lupin protein had hypocholesterolemic effects in laboratory animals. However, the effect in humans has not been elucidated till now.
Aim of the study
To investigate the effect of lupin protein on circulating cholesterol in plasma and lipoproteins of hypercholesterolemic subjects.
Subjects and methods
A randomised, double-blind, placebo-controlled, parallel trial (23 females and 20 males completed the trial) was conducted to compare the effects of lupin protein versus casein as control protein on plasma lipids and amino acids. Thirty-five grams of the test protein were consumed daily for 6 weeks.
Both lupin protein and casein resulted in a reduction of circulating plasma cholesterol (−0.50 ± 0.64 and −0.47 ± 0.79 mM; P < 0.05) from baseline to week 6. The reduction of plasma cholesterol was mainly caused by a reduction of LDL cholesterol in the lupin protein group (−0.31 ± 0.46 mM; P < 0.05), while in the casein group HDL cholesterol significantly declined (−0.17 ± 0.15 mM; P < 0.05). Comparing the lupin protein group with the casein group yielded a difference in the net changes from baseline to week 6 in the LDL:HDL cholesterol-ratio of −0.24 (95% CI: −0.007, −0.479; P < 0.05). No significant differences in net changes were observed for plasma concentrations of triglycerides, glucose, homocysteine, taurine and most of the amino acids.
Lupin protein compared to casein slightly lowered the concentration of LDL cholesterol in hypercholesterolemic subjects, without altering HDL cholesterol. No or minor effects of lupin protein were observed on circulating glucose, homocysteine and plasma amino acids.
KeywordsLupin protein Plasma lipids Hypercholesterolemic subjects
We thank Fraunhofer-Institute (Freising, Germany) for producing the protein bars and Dr. Petra Pavel and Karin Richter for their help in blood collection for this study. The research was supported by a grant from the Bundesministerium für Wirtschaft und Technologie (BMWi).
- 4.Food and Drug Administration (1999) Food labeling, health claims, soy protein, and coronary heart disease. Fed Regist 57:699–733Google Scholar
- 5.Horton JD, Goldstein JL, Brown MS (2002) SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest 109:1125–1131Google Scholar
- 10.Nagata C, Shimizu H, Takami R, Hayashi M, Takeda N, Yasuda K (2003) Soy product intake is inversely associated with serum homocysteine level in premenopausal Japanese women. J Nutr 133:797–800Google Scholar
- 11.Petterson DS (1998) Composition and food uses of lupins. In: Gladstones JS, Atkins CA, Hamblin J (eds) Lupins as crop plants: biology, production and utilization. CAB International, Wallingford, UK, pp 353–384Google Scholar
- 14.Sirtori CR, Lovati MR, Manzoni C, Castiglioni S, Duranti M, Magni C, Morandi S, D’Agostina A, Arnoldi A (2004) Proteins of white lupin seed, a naturally isoflavone-poor legume, reduce cholesterolemia in rats and increase LDL receptor activity in HepG2 cells. J Nutr 134:18–23Google Scholar
- 15.Sirtori CR, Lovati MR, Manzoni C, Gianazza E, Bondioli A, Staels B, Auwerx J (1998) Reduction of serum cholesterol by soybean proteins: clinical experience and potential molecular mechanisms. Nutr Metab Cardiovasc Dis 8:334–340Google Scholar
- 18.Tonstad S, Smerud K, Høie L (2002) A comparison of the effects of 2 doses of soy protein or casein on serum lipids, serum lipoproteins, and plasma total homocysteine in hypercholesterolemic subjects. Am J Clin Nutr 76:78–84Google Scholar
- 19.Tovar AR, Murguía F, Cruz C, Hernández-Pando R, Aguilar-Salinas CA, Pedraza-Chaverri J, Correa-Rotter R, Torres N (2002) A soy protein diet alters hepatic lipid metabolism gene expression and reduces serum lipids and renal fibrogenic cytokines in rats with chronic nephrotic syndrome. J Nutr 132:2562–2569Google Scholar
- 20.Vanuzzo D, Pilotto L, Mirolo R, Pirelli S (2008) Cardiovascular risk and cardiometabolic risk: and epidemiological evaluation. G Ital Cardiol 9:6S–17SGoogle Scholar
- 21.Vester B, Rasmussen K (1991) High performance liquid chromatography method for rapid and accurate determination of homocysteine in plasma and serum. Eur J Clin Chem Clin Biochem 29:549–554Google Scholar
- 23.Yuan G, Al-Shali KZ, Hegele RA (2007) Hypertriglyceridemia: its etiology, effects and treatment. CMAJ 176:1113–1120Google Scholar