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European Journal of Nutrition

, Volume 48, Issue 4, pp 235–242 | Cite as

Methanolic extract of onion (Allium cepa) attenuates ischemia/hypoxia-induced apoptosis in cardiomyocytes via antioxidant effect

  • Sok Park
  • Mi-Young Kim
  • Dong Ha Lee
  • Soo Hwan Lee
  • Eun Joo Baik
  • Chang-Hyun Moon
  • Se Won Park
  • Eun Young Ko
  • Sei-Ryang Oh
  • Yi-Sook JungEmail author
Original Contribution

Abstract

Background

Although there is growing awareness of the beneficial potential of onion intake to lower the risk of cardiovascular disease, there is little information about the effect of onion on ischemic heart injury, one of the most common cardiovascular diseases.

Aim of the study

This study investigates the effect of the methanol-soluble extract of onion on ischemic injury in heart-derived H9c2 cells in vitro and in rat hearts in vivo. The underlying mechanism is also investigated.

Methods

To evaluate the effect of onion on ischemia-induced cell death, LDH release and TUNEL-positivity were assessed in H9c2 cells, and the infarct size was measured in a myocardial infarct model. To investigate the mechanism of the cardioprotection by onion, the reactive oxygen species (ROS) level and the mitochondrial membrane potential (ΔΨm) were measured using an imaging technique; the caspase-3 activity was assayed, and Western blotting was performed to examine cytochrome c release in H9c2 cells.

Results

The methanolic extract of onion had a preventive effect on ischemia/hypoxia-induced apoptotic death in H9c2 cells in vitro and in rat heart in vivo. The onion extract (0.05 g/ml) inhibited the elevation of the ROS, mitochondrial membrane depolarization, cytochrome c release and caspase-3 activation during hypoxia in H9c2 cells. In the in vivo rat myocardial infarction model, onion extract (10 g/kg) significantly reduced the infarct size, the apoptotic cell death of the heart and the plasma MDA level.

Conclusion

In conclusion, the results of this study suggest that the methanolic extract of onion attenuates ischemia/hypoxia-induced apoptosis in heart-derived H9c2 cells in vitro and in rat hearts in vivo, through, at least in part, an antioxidant effect.

Keywords

Onion extract Antioxidant Cardioprotection Ischemic injury Apoptosis 

Notes

Acknowledgments

This study was supported by Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, and by “Specific Joint Agricultural Research-promoting Projects (20080401-033-302-001-02-00)”, RDA, Republic of Korea. This work was also supported by (2004) grant from “Department of Medical Sciences, The Graduate School, Ajou University”, and by a grant (CBM32-B3003-01-02-00) from the center for Biological Modulators of the 21st Century Frontier R&D Program of the Ministry of Science and Technology.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sok Park
    • 1
  • Mi-Young Kim
    • 1
    • 2
  • Dong Ha Lee
    • 1
    • 2
  • Soo Hwan Lee
    • 1
  • Eun Joo Baik
    • 1
  • Chang-Hyun Moon
    • 1
  • Se Won Park
    • 3
  • Eun Young Ko
    • 3
  • Sei-Ryang Oh
    • 4
  • Yi-Sook Jung
    • 1
    • 2
    Email author
  1. 1.Department of Physiology, School of MedicineAjou UniversitySuwonSouth Korea
  2. 2.Department of Molecular Science and TechnologyAjou UniversitySuwonSouth Korea
  3. 3.College of Life and Environmental SciencesKonkuk UniversitySeoulSouth Korea
  4. 4.Natural Medicine Research CenterKRIBBDaejeonSouth Korea

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