Effects of high-AGE beverage on RAGE and VEGF expressions in the liver and kidneys
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The formation and accumulation of advanced glycation end products (AGEs) increase in some lifestyle-related diseases as well as in aging; however, little is known about the relationship between food-derived AGEs and the pathology of such diseases.
Aim of the study and methods
To explore whether food items containing high levels of AGEs are involved in the development of lifestyle-related diseases, rats were orally administered a commercial high-AGE beverage [Lactobacillus beverage-A (LB-A)]. With a particular focus on angiogenesis-associated diseases, the gene expressions of vascular endothelial growth factor (VEGF) and the receptor for AGEs (RAGE) were examined in the liver and kidneys using real-time reverse transcription-polymerase chain reaction. Moreover, AGE deposition was immunohistochemically investigated in these tissues.
Results and conclusions
Hepatic VEGF expression was significantly increased in rats administered LB-A (P < 0.01 vs. control). Furthermore, immunohistochemical analysis detected glucose-derived AGE-positive cells in the liver from the LB-A group. These results suggest that AGE-rich beverages increase hepatic VEGF expression and AGE accumulation, bringing about early events associated with lifestyle-related diseases.
KeywordsLactobacillus beverage food-derived AGEs lifestyle-related diseases VEGF RAGE
This work was supported in part by a grant from the Japan Society for the Promotion of Science (Grant-in-Aid for Scientific Research [B], #19300254) to Masayoshi Takeuchi and by the Specific Research Fund of Hokuriku University (category B, code No. 230007, FY2007) to Takashi Sato and Masayoshi Takeuchi.
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