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European Journal of Nutrition

, Volume 47, Issue 4, pp 217–223 | Cite as

Does the biomarker 15N-lactose ureide allow to estimate the site of fermentation of resistant starch?

  • Lieselotte Cloetens
  • Vicky De Preter
  • Henriette De Loor
  • Paul Rutgeerts
  • Kristin VerbekeEmail author
ORIGINAL CONTRIBUTION

Abstract

We evaluated the effect of resistant starch (RS) and resistant starch with wheat bran (RS+WB) on the colonic ammonia metabolism in healthy volunteers using the biomarker 15N-lactose ureide (15N-LU). Particularly, it was investigated whether this biomarker allowed to estimate differences in the site of fermentation. Ten volunteers were included in a placebo-controlled crossover study. They consumed in random order 2 × 15 g RS/day for 2 weeks and placebo for 2 weeks separated by 2 weeks wash-out. At baseline, on the first day of each intake period and after each intake period, they consumed a 15N-labelled test meal and collected all urine in different fractions for 48 h. In ten other volunteers, the effect of 2 × 15 g RS/day and of 2 × 15 g RS  + 2 × 6 g WB was compared. These volunteers collected urine and feces for 72 h. 15N-content of urine and feces was measured using combustion-isotope ratio mass spectrometry. RS exerted a significant decrease in cumulative urinary 15N-excretion which was different from placebo. The effect was most pronounced in the 6–24 h urine fraction which suggest fermentation in the proximal colon. The effect of RS+WB on cumulative urinary 15N-excretion was not significantly different from the effect of RS. A less pronounced decrease in the 6–24 h fraction was observed suggesting less fermentation in the proximal colon whereas no indications for more distal fermentation were observed. Since about 80% of the cumulative urinary 15N was recovered within 24 h, it was concluded that the biomarker 15N-LU was useful to monitor processes in the proximal colon rather than in the distal colon.

Keywords

stable isotope prebiotic wheat bran resistant starch site of fermentation 

Notes

Acknowledgments

The present study was carried out with financial support from the Commission of the European Communities, specific RTD programme “Quality of Life and Management of Living Resources”, QLK1-2001-00431 “Stable isotope applications to monitor starch digestion and fermentation for the development of functional foods”. It does not necessarily reflect its views and in no way anticipates the Commission’s future policy in this area.

References

  1. 1.
    Bouhnik Y, Raskine L, Simoneau G, Vicaut E, Neut C, Flourie B, Brouns F, Bornet FR (2004) The capacity of nondigestible carbohydrates to stimulate fecal bifidobacteria in healthy humans: a double-blind, randomized, placebo-controlled, parallel-group, dose-response relation study. Am J Clin Nutr 80:1658–1664Google Scholar
  2. 2.
    Brouns F, Kettlitz B, Arrigoni E (2002) Resistant starch and “the butyrate revolution”. Trends Food Sci Technol 13:251–261CrossRefGoogle Scholar
  3. 3.
    Cummings JH (1997) The large intestine in nutrition and disease. Brussels, Institut Danone. Danone Chair Monograph 3Google Scholar
  4. 4.
    Cummings JH, Beatty ER, Kingman SM, Bingham SA, Englyst HN (1996) Digestion and physiological properties of resistant starch in the human large bowel. Br J Nutr 75:733–747CrossRefGoogle Scholar
  5. 5.
    De Preter V, Geboes K, Verbrugghe K, De Vuyst L, Vanhoutte T, Huys G, Swings J, Pot B, Verbeke K (2004) The in vivo use of the stable isotope-labelled biomarkers lactose-[15N]ureide and [2H4]tyrosine to assess the effects of pro- and prebiotics on the intestinal flora of healthy human volunteers. Br J Nutr 92:439–446CrossRefGoogle Scholar
  6. 6.
    De Preter V, Vanhoutte T, Huys G, Swings J, De Vuyst L, Rutgeerts P, Verbeke K (2007) Effects of Lactobacillus casei Shirota, Bifidobacterium breve, and oligofructose-enriched inulin on colonic nitrogen-protein metabolism in healthy humans. Am J Physiol Gastrointest Liver Physiol 292:G358–G368CrossRefGoogle Scholar
  7. 7.
    De Preter V, Vanhoutte T, Huys G, Swings J, Rutgeerts P, Verbeke K (2006) Effect of lactulose and Saccharomyces boulardii administration on the colonic urea-nitrogen metabolism and the bifidobacteria concentration in healthy human subjects. Aliment Pharmacol Ther 23:963–974CrossRefGoogle Scholar
  8. 8.
    De Preter V, Cloetens L, Rutgeerts P, Verbeke K (2007) Influence of resistant starch alone or combined with wheat bran on gastric emptying and protein digestion in healthy volunteers. Scand J Gastroenterol 42:1187–1193CrossRefGoogle Scholar
  9. 9.
    Englyst HN, Hay S, Macfarlane GT (1987) Polysaccharide breakdown by mixed populations of human faecal bacteria. FEMS Microbiol Lett 45:163–171CrossRefGoogle Scholar
  10. 10.
    Geboes KP, De Hertogh G, De PV, Luypaerts A, Bammens B, Evenepoel P, Ghoos Y, Geboes K, Rutgeerts P, Verbeke K (2006) The influence of inulin on the absorption of nitrogen and the production of metabolites of protein fermentation in the colon. Br J Nutr 96:1078–1086Google Scholar
  11. 11.
    Geboes KP, De Preter V, Luypaerts A, Bammens B, Evenepoel P, Ghoos Y, Rutgeerts P, Verbeke K (2005) Validation of lactose[15N,15N]ureide as a tool to study colonic nitrogen metabolism. Am J Physiol Gastrointest Liver Physiol 288:G994–G999CrossRefGoogle Scholar
  12. 12.
    Govers MJ, Gannon NJ, Dunshea FR, Gibson PR, Muir JG (1999) Wheat bran affects the site of fermentation of resistant starch and luminal indexes related to colon cancer risk: a study in pigs. Gut 45:840–847CrossRefGoogle Scholar
  13. 13.
    Henningsson AM, Bjorck IME, Nyman EM (2002) Combinations of indigestible carbohydrates affect short-chain fatty acid formation in the hindgut of rats. J Nutr 132:3098–3104Google Scholar
  14. 14.
    Hofmann E (1931) Ueber den Abbau von glucoseureid durch bakterien. Biochem Zeitschr 243:416–422Google Scholar
  15. 15.
    Jackson AA, Bundy R, Hounslow A, Murphy JL, Wootton SA (1999) Metabolism of lactose-[C-13]ureide and lactose-[N-15,N-15]ureide in normal adults consuming a diet marginally adequate in protein. Clin Sci 97:547–555CrossRefGoogle Scholar
  16. 16.
    Metcalf AM, Phillips SF, Zinsmeister AR, MacCarty RL, Beart RW, Wolk A (1987) Simplified assessment of segmental colonic transit. Gastroenterology 92:40–47Google Scholar
  17. 17.
    Morita T, Kasaoka S, Hase K, Kiriyama S (1999) Psyllium shifts the fermentation site of high-amylose cornstarch toward the distal colon and increases fecal butyrate concentration in rats. J Nutr 129:2081–2087Google Scholar
  18. 18.
    Morrison DJ, Dodson B, Preston T, Weaver LT (2001) Rapid quality control analysis of C-13—enriched substrate synthesis by isotope ratio mass spectrometry. Rapid Commun Mass Spectrom 15:1279–1282CrossRefGoogle Scholar
  19. 19.
    Muir JG, Walker KZ, Kaimakamis MA, Cameron MA, Govers MJ, Lu ZX, Young GP, O’Dea K (1998) Modulation of fecal markers relevant to colon cancer risk: a high-starch Chinese diet did not generate expected beneficial changes relative to a Western-type diet. Am J Clin Nutr 68:372–379Google Scholar
  20. 20.
    Muir JG, Yeow EG, Keogh J, Pizzey C, Bird AR, Sharpe K, O’Dea K, Macrae FA (2004) Combining wheat bran with resistant starch has more beneficial effects on fecal indexes than does wheat bran alone. Am J Clin Nutr 79:1020–1028Google Scholar
  21. 21.
    Ruemmele FM, Heine WE, Keller KM, Lentze MJ (1997) Metabolism of glycosyl ureides by human intestinal brush border enzymes. Biochim Biophys Acta Gen Subj 1336:275–280CrossRefGoogle Scholar
  22. 22.
    Schoorl MN (1903) Les ureides (carbamides) des sucres. Recl Trav Chim 22:1Google Scholar
  23. 23.
    Topping DL, Clifton PM (2001) Short-chain fatty acids and human colonic function: Roles of resistant starch and nonstarch polysaccharides. Physiol Rev 81:1031–1064Google Scholar
  24. 24.
    Vandokkum W, Pikaar NA, Thissen JTNM (1983) Physiological-effects of fiber-rich types of Bread .2. Dietary fiber from Bread—digestibility by the intestinal microflora and water-holding capacity in the colon of human-subjects. Br J Nutr 50:61–74CrossRefGoogle Scholar
  25. 25.
    Yao HT, Chiang MT (2006) Chitosan shifts the fermentation site toward the distal colon and increases the fecal short-chain fatty acids concentrations in rats. Int J Vitam Nutr Res 76:57–64CrossRefGoogle Scholar

Copyright information

© Spinger 2008

Authors and Affiliations

  • Lieselotte Cloetens
    • 1
  • Vicky De Preter
    • 1
  • Henriette De Loor
    • 1
  • Paul Rutgeerts
    • 1
  • Kristin Verbeke
    • 1
    • 2
    Email author
  1. 1.Dept. of Gastrointestinal ResearchKatholieke Universiteit LeuvenLeuvenBelgium
  2. 2.Dept. of Gastrointestinal ResearchUniversity Hospital LeuvenLeuvenBelgium

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