Changes in predictors and status of homocysteine in young male adults after a dietary intervention with vegetables, fruits and bread
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Elevated plasma total homocysteine (p-tHcy) is associated with increased risk of cardiovascular disease, and an inverse association has been shown between the dietary intake of B-vitamins, B-vitamin profile and the concentration of p-tHcy.
Aim of the study
The main objective of this investigation was to study the effect of a dietary intervention focusing on an increased intake of vegetables, fruits and bread. The effect of the dietary intervention was determined by the changes in plasma concentrations of tHcy, cysteine (cys), riboflavin, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) and serum concentrations of folate and vitamin B12.
An intervention study with duration of 5 months, including 541 male recruits from the Norwegian National Guard, Vaernes and a control group, including 209 male recruits from the Norwegian Army, Heggelia.
The dietary intervention resulted in decreased concentration of p-tHcy (−10%, P = 0.002), p-cys (−6%, P < 0.001) and FMN (−11%, P = 0.310) and increased concentration of riboflavin (+23%, P < 0.001) and FAD (+10%, P = 0.008) in the intervention group compared with the control group. The change in p-tHcy concentration was positively related to the change in the concentration of p-cys (P < 0.001) and FMN (P = 0.035) and inversely related to the change in concentration of folate (P = 0.021).
A dietary intervention program focusing on an increased intake of vegetables, fruits and bread showed a favourable effect on the concentration of p-tHcy and its metabolites. Our findings suggest that the changes in the concentration of p-cys, folate and FMN seem to be predictors of changes in the p-tHcy concentration.
Keywordsdietary intervention homocysteine B-vitamins young men
This study was funded by the Norwegian Research Council. The authors want to thank the personnel at The Norwegian National Guard, Værnes and the Norwegian Army, Heggelia for support and positive attitude regarding data collections. We also want to thank Madelene Johansson for participating in the data collection of the control group and Betzy Kvarme and Elin Skarland Frøyland for their help with analyses of p-tHcy and riboflavin, FAD and FMN in plasma.
- 1.Appel LJ, Miller ER 3rd, Jee SH, Stolzenberg-Solomon R, Lin PH, Erlinger T, Nadeau MR, Selhub J (2000) Effect of dietary patterns on serum homocysteine: results of a randomized, controlled feeding study. Circulation 102(8):852–857Google Scholar
- 3.Bates CJ, Mansoor MA, Gregory J, Pentieva K, Prentice A (2002) Correlates of plasma homocysteine, cysteine and cysteinyl-glycine in respondents in the British national diet and nutrition survey of young people aged 4–18 years, and a comparison with the survey of people Aged 65 years and over. Br J Nutr 87(1):71–79CrossRefGoogle Scholar
- 4.Becker W (1999) Vilka är källorna till våra näringsämnen? Vår Föda 3:16–20Google Scholar
- 9.Brody T (1994) Nutritional biochemistry. Academic Press, San DiegoGoogle Scholar
- 10.Broekmans WMR, Klopping-Ketelaars IAA, Schuurman CRWC, Verhagen H, van den Berg H, Kok FJ, van Poppel G (2000) Fruits and vegetables increase plasma carotenoids and vitamins and decrease homocysteine in humans. J Nutr 130(6):1578–1583Google Scholar
- 11.Brouwer IA, van Dusseldorp V, West CE, Meyboom S, Thomas CM, Duran M, van het Hof KH, Eskes TK, Hautvast JG, Steegers-Theunissen RP (1999) Dietary folate from vegetables and citrus fruit decreases plasma homocysteine concentrations in humans in a dietary controlled trial. J Nutr 129(6):1135–1139Google Scholar
- 15.Ganji V, Kafai MR (2003) Demographic, health, lifestyle, and blood vitamin determinants of serum total homocysteine concentrations in the third national health and nutrition examination survey, 1988–1994. Am J Clin Nutr 77(4):826–833Google Scholar
- 16.Ganji V, Kafai MR (2004) Frequent consumption of milk, yogurt, cold breakfast cereals, peppers, and cruciferous vegetables and intakes of dietary folate and riboflavin but not vitamins B-12 and B-6 are inversely associated with serum total homocysteine concentrations in the US population. Am J Clin Nutr 80(6):1500–1507Google Scholar
- 21.Hustad S, Ueland PM, Vollset SE, Zhang Y, Bjorke-Monsen AL, Schneede J (2000) Riboflavin as a determinant of plasma total homocysteine; effect modification by the methylenetetrahydrofolate reductase C677T polymorphism. Clin Chem 46(8):1065–1067Google Scholar
- 22.Jacques PF, Bostom AG, Williams RR, Ellison RC, Eckfeldt JH, Rosenberg IH, Selhub J, Rozen R (1996) Relation between folate status, a common mutation in methylenetetrahydrofolate reductase, and plasma homocysteine concentrations. Circulation 93(1):7–9Google Scholar
- 23.Jacques PF, Bostom AG, Wilson PWF, Rich S, Rosenberg IH, Selhub J (2001) Determinants of plasma total homocysteine concentration in the Framingham offspring cohort. Am J Clin Nutr 73(3):613–621Google Scholar
- 24.Jacques PF, Kalmbach R, Bagley PJ, Russo GT, Rogers G, Wilson PW, Rosenberg IH, Selhub J (2002) The relationship between riboflavin and plasma total homocysteine in the Framingham offspring cohort is influenced by folate status and the C677T transition in the methylenetetrahydrofolate reductase gene. J Nutr 132(2):283–288Google Scholar
- 26.Lonn E, Yusuf S, Arnold MJ, Sheridan P, Pogue J, Micks M, McQueen MJ, Probstfield J, Fedor G, Held C, Genest J Jr, Heart Outcomes Prevention Evaluation (HOPE) 2 Investigators (2006) Homocysteine lowering with folic acid and b vitamins in vascular disease. N Engl J Med 354(15):1567–1577CrossRefGoogle Scholar
- 27.Mansoor MA, Bergmark C, Svardal AM, Lønning PE, Ueland PM (1995) Redox status and protein binding of plasma homocysteine and other aminothiols in patients with early-onset peripheral vascular disease. Homocysteine and peripheral vascular disease. Arteriscler Thromb Vasc Biol 15(2):232–240Google Scholar
- 29.McKinley MC, McNulty H, McPartlin J, Strain JJ, Pentieva K, Ward M, Weir DG, Scott JM (2001) Low-dose vitamin B-6 effectively lowers fasting plasma homocysteine in healthy elderly persons who are folate and riboflavin replete. Am J Clin Nutr 73(4):759–764Google Scholar
- 34.Moore SE, Mansoor MA, Bates CJ, Prentice AM (2006) Plasma homocysteine, folate and vitamin B(12) compared between rural Gambian and UK adults. Br J Nutr 96(3):508–515Google Scholar
- 37.Refsum H, Nurk E, Smith AD, Ueland PM, Gjesdal CG, Bjelland I, Tverdal A, Tell GS, Nygard O, Vollset SE (2006) The hordaland homocysteine study: a community-based study of homocysteine, its determinants, and associations with disease. J Nutr 136(6):1731–1740Google Scholar
- 38.Rowley KG, Su Q, Cincotta M, Skinner M, Skinner K, Pindan B, White GA, O’Dea K (2001) Improvements in circulating cholesterol, antioxidants, and homocysteine after dietary intervention in an Australian aboriginal community. Am J Clin Nutr 74(4):442–448Google Scholar
- 40.Selhub J, Jacques PF, Bostom AG, Wilson PW, Rosenberg IH (2000) Relationship between plasma homocysteine and vitamin status in the Framingham study population. Impact of folic acid fortification. Public Health Rev 28(1–4):117–145Google Scholar
- 42.The Norwegian directorate for health and social affairs (2006) Utviklingen i norsk kosthold. Matforsyningsstatistikk og forbrukerundersøkelser. Oslo, pp 1–103Google Scholar
- 43.Ueland PM, Refsum H, Stabler SP, Malinow MR, Andersson A, Allen RH (1993) Total homocysteine in plasma or serum: methods and clinical applications. Clin Chem 39(9):1764–1779Google Scholar
- 45.Venn BJ, Mann JI, Williams SM, Riddell LJ, Chisholm A, Harper MJ, Aitken W (2002) Dietary counseling to increase natural folate intake: a randomized, placebo-controlled trial in free-living subjects to assess effects on serum folate and plasma total homocysteine. Am J Clin Nutr 76(4):758–765Google Scholar
- 48.Zimmermann MB, Shane B (1993) Supplemental folic acid. Am J Clin Nutr 58(2):127–128Google Scholar