European Journal of Nutrition

, Volume 47, Issue 4, pp 192–200 | Cite as

Comparison of enzymatically synthesized inulin, resistant maltodextrin and clofibrate effects on biomarkers of metabolic disease in rats fed a high-fat and high-sucrose (cafeteria) diet

  • Junko Sugatani
  • Makoto Osabe
  • Tadashi Wada
  • Kasumi Yamakawa
  • Yasuhiro Yamazaki
  • Tadanobu Takahashi
  • Akira Ikari
  • Masao MiwaEmail author



While naturally occurring inulin has anti-hyperlipidemic effects in animals and humans, health effects of synthetic inulin with different degrees of fructose polymerization remain poorly understood.

Aim of the study

Our study aimed at distinguishing health effects of synthetic inulin with different degrees of fructose polymerization (DP) from those of resistant maltodextrin and clofibrate.


We examined effects of synthetic inulin on serum and liver lipid profiles and blood biochemical parameters in rats fed a high-fat and high-sucrose (HF, cafeteria) diet when compared to resistant maltodextrin and clofibrate.


Treatment with inulin (average DP = 6–8, 16–17 and 23) and resistant maltodextrin for 3 weeks reduced the elevation in liver levels of triacylglycerol and total cholesterol of rats fed the cafeteria diet but not the standard diet. In these groups, inulin (average DP = 16–17) significantly reduced the portal plasma glucose level. Moreover, the levels of portal plasma propionate and circulating serum adiponectin, which were decreased in cafeteria rats, recovered to nearly normal levels after administration of inulin (average DP  = 16–17). In addition, the dietary inulin suppressed elevation in levels of portal plasma insulin and circulating serum leptin and induction of acetyl-CoA carboxylase and fatty acid synthase mRNAs in the liver of cafeteria rats, consistent with the reduction of liver lipids. The dietary inulin and clofibrate markedly reduced triacylglycerol levels in serum very low density lipoprotein (VLDL) and liver and epididymal adipose tissue weights of cafeteria rats; the extent of suppression by the dietary inulin was higher than that by clofibrate. No additive or synergistic effect of the dietary inulin and clofibrate was found in decrease in circulating serum VLDL and liver lipid levels.


These observations indicate that the dietary inulin may prevent the development of metabolic disease such as hyperlipidemia and hyperinsulinemia caused by intake of cafeteria diet, in association with suppression of liver lipogenesis.


synthetic inulin anti-metabolic disease clofibrate propionate glucose 



Aspartate aminotransferase


Alanin aminotransferase


Carbohydrate responsive element-binding protein


Degree of polymerization

HMG-CoA reductase

3–hydroxy-3-methylglutaryl CoA reductase


Peroxisome proliferator- activated receptor


Sterol regulatory element-binding protein


White adipose tissue



We gratefully acknowledge Satoko Ishikawa, Tomoaki Fukuyama, Shiho Taniguchi, Akihiro Oumi, and Shuji Kamata for excellent technical assistance.

Supplementary material

394_2008_713_Fig3_ESM.jpg (144 kb)
Supplementary material (ESM1 144 kb)


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Copyright information

© Spinger 2008

Authors and Affiliations

  • Junko Sugatani
    • 1
    • 2
  • Makoto Osabe
    • 1
  • Tadashi Wada
    • 3
  • Kasumi Yamakawa
    • 1
  • Yasuhiro Yamazaki
    • 1
  • Tadanobu Takahashi
    • 1
  • Akira Ikari
    • 1
  • Masao Miwa
    • 1
    Email author
  1. 1.Dept. of Pharmaco-Biochemistry, School of Pharmaceutical SciencesUniversity of ShizuokaShizuoka CityJapan
  2. 2.Global Center of Excellence for Innovation in Human Health Sciences, School of Pharmaceutical SciencesUniversity of ShizuokaShizuokaJapan
  3. 3.Fuji Nihon Seito CorporationShizuokaJapan

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