Implications of oxidative stress in high sucrose low magnesium diet fed rats
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Magnesium deficiency as well as excess sucrose in the diet have been shown to be associated with the generation of reactive oxygen species (ROS).
Aim of the study
In the present study we have investigated the combined effect of low magnesium high sucrose diet on the development of oxidative stress in rats.
Male Wistar rats were divided into four groups and fed control (C), low magnesium (LM), high sucrose (HS) and low magnesium high sucrose (HSLM) diet for a period of 3 months. Levels of various antioxidants, viz. ascorbic acid, vitamin E, uric acid, glutathione and non-protein thiols were determined along with malondialdehyde levels (lipid peroxidation marker). Anti-oxidant enzyme activities were determined in livers of experimental diet fed animals.
Compared to controls, significantly increased lipid peroxidation was observed in plasma and liver tissue of animals in the three experimental groups, however, the combined HSLM group showed greater lipid peroxidation. Levels of various antioxidants fell significantly in plasma and tissue of LM, HS and HSLM rats. Total thiols as well as liver non-protein thiols followed a similar trend with the greatest drop in anti-oxidant potential seen in the HSLM rats. The activities of the anti-oxidant enzymes viz. SOD, GST and catalase also declined considerably in test animals w.r.t controls, with the HSLM group showing the lowest activities.
These findings suggest that a diet low in magnesium and high in sucrose causes oxidative stress in rats, as reflected by increased lipid peroxidation and reduced anti-oxidant potential.
Keywordssucrose low magnesium oxidative stress lipid peroxidation anti- oxidant enzymes
- 2.Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888Google Scholar
- 5.Bruegere CM, Nowacki W, Gueux E, Keryszko J, Rock E, Rayssigiuer Y, Mazur A (1991) Accelerated thymus involution in magnesium deficient rats in related to enhanced apoptosis and sensitivity to oxidative stress. Br J Nutr 81:405Google Scholar
- 7.Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310Google Scholar
- 10.Chaudhary DP, Boparai RK, Sharma R, Bansal DD (2004) Studies on the development of an insulin resistance rat model by chronic feeding of high sucrose low magnesium diet. Magnesium Res 17:293–300Google Scholar
- 13.El Hindi HM, Amer HA (1989) Effect of thiamine, magnesium and sulphate salts on growth, thiamine levels and serum lipid constituents in rats. J Nutr Sci Vitaminol 35:505–510Google Scholar
- 15.Faure P, Rossini E, Lafond JL, Richard MJ, Favier A, Halimi S (1997) Vitamin E improves free radical defense system potential and insulin sensitivity of rats fed high fructose diet. J Nutr 127:103–107Google Scholar
- 16.Frankel EN, Neff WE (1983) Formation of malondialdehyde from lipid peroxidation products. Biochim Biophys Acta 754:264–270Google Scholar
- 17.Habig WH, Pabst MJ, Kakoby WB (1974) Glutathione-S-transferase. J Biol Chem 248:7130–7139Google Scholar
- 18.Halliwell B (1996) Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional anti-oxidant intake in humans. Free Radic Res 25:57–74Google Scholar
- 19.Hans CP, Chaudhary DP, Bansal DD (2002) Magnesium deficiency increases oxidative stress in rats. Ind J Exp Biol 40:1275–1279Google Scholar
- 20.Hollenbeck CB, Coulston AM, Reaven GM (1986) Glycemic effects of carbohydrates: a different perspective. Diabetes Care 9:641–647Google Scholar
- 21.Hsu JM, Smith JC, Yunice AA, Kepford G (1983) Impairment of ascorbic acid synthesis in liver extracts of magnesium deficient rats. J Nutr 113:2041–2047Google Scholar
- 23.Koster JF, Biemond P, Swaak AJ (1986) Intracellular and extracellular sulfhydryl levels in rheumatoid arthritis. Annu Rheum Dis 45:44–46Google Scholar
- 24.Levi BC, Bermer MJ (1998) Long term fructose consumption accelerate glycation and several age related variables in male rats. J Nutr 128:1442–1449Google Scholar
- 25.Luck H (1971) In: Bergmeyer HU (ed) Methods in enzymatic analysis, vol III. Academic Press, New York, pp 279Google Scholar
- 26.Martinek RG (1964) Methods for estimation of vitamin E (α-tocopherol) in serum. Clin Chem 10:1078–1086Google Scholar
- 27.McDonald RB (1995) Influence of dietary sucrose on biological ageing. Am J Clin Nutr 62:284S–293SGoogle Scholar
- 29.Rayssiguier Y, Durlach J, Gueux E, Rock E, Mazur A (1993) Magnesium and ageing. 1 Experimental data; importance of oxidative damage. Magnesium Res 6:369–378Google Scholar
- 30.Rayssiguier Y, Gueux E, Bussiere L, Mazur A (1993) Copper deficiency increases the susceptibility of lipoproteins and tissues to peroxidation in rats. J Nutr 123:1343–1348Google Scholar
- 31.Rayssiguier Y, Gueux E, Wieser D (1981) Effect of magnesium deficiency on lipid metabolism in rats fed a high carbohydrate diet. J Nutr 111:1876–1883Google Scholar
- 32.Reiser S, Michaelis OV, Putney J, Hallfrisch J (1975) Effect of sucrose feeding on intestinal transport of sugars in two strains of rats. J Nutr 105:894–905Google Scholar
- 33.Rice-Evans CA, Diplack AT, Symons MCR (1991) In: Burden RH, van Knippenber PH (eds) Amsterdam, ElsevierGoogle Scholar
- 34.Roe RC, Kuether D (1943) Determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenyhydrazine derivatives of dehydroascorbic acid. J Biol Chem 147:399–407Google Scholar
- 35.Rosan GM, Pou S, Ramos CL, Cohen MS, Britigan BE (1995) Free radicals and phagocytic cells. FASEB J 9:200–209Google Scholar
- 38.Szezeklik A, Gryglewski RJ, Demagala R, Dworski R, Basista M (1985) Dietary supplementation with vitamin E in hyperlipopropteinemias; effect on plasma lipid peroxides, antioxidant activity, prostacyclin generation and platelet aggregability. Thromb Haemost 54:425–430Google Scholar
- 39.Thefeld W, et al. (1973) DTSCH. Medws chr 98380Google Scholar
- 40.Thomas SR, Neuzil J, Mohar D, Stocker R (1995) Restoration of tocopherol by co-incubation makes (-tocopherol an effective anti-oxidant for low density lipoproteins. Am J Clin Nutr 62:S1357Google Scholar
- 41.Thuvasethakul P, Wajjwalku W (1987) Serum magnesium determined by use of methyl thymol blue. Clin Chem 33:614–615Google Scholar
- 43.Wolf SP, Dean RT (1987) Glucose auto-oxidation and protein modification. The potential role of anti-oxidation glycosylation in diabetes. Biochem J 245:243–250Google Scholar