Effects of hempseed and flaxseed oils on the profile of serum lipids, serum total and lipoprotein lipid concentrations and haemostatic factors
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Both hempseed oil (HO) and flaxseed oil (FO) contain high amounts of essential fatty acids (FAs); i.e. linoleic acid (LA, 18:2n-6) and alpha-linolenic acid (ALA, 18:3n-3), but almost in opposite ratios. An excessive intake of one essential FA over the other may interfere with the metabolism of the other while the metabolisms of LA and ALA compete for the same enzymes. It is not known whether there is a difference between n-3 and n-6 FA of plant origin in the effects on serum lipid profile.
Aim of the study
To compare the effects of HO and FO on the profile of serum lipids and fasting concentrations of serum total and lipoprotein lipids, plasma glucose and insulin, and haemostatic factors in healthy humans.
Fourteen healthy volunteers participated in the study. A randomised, double-blind crossover design was used. The volunteers consumed HO and FO (30 ml/day) for 4 weeks each. The periods were separated by a 4-week washout period.
The HO period resulted in higher proportions of both LA and gamma-linolenic acid in serum cholesteryl esters (CE) and triglycerides (TG) as compared with the FO period (P < 0.001), whereas the FO period resulted in a higher proportion of ALA in both serum CE and TG as compared with the HO period (P < 0.001). The proportion of arachidonic acid in CE was lower after the FO period than after the HO period (P < 0.05). The HO period resulted in a lower total-to-HDL cholesterol ratio compared with the FO period (P = 0.065). No significant differences were found between the periods in measured values of fasting serum total or lipoprotein lipids, plasma glucose, insulin or hemostatic factors.
The effects of HO and FO on the profile of serum lipids differed significantly, with only minor effects on concentrations of fasting serum total or lipoprotein lipids, and no significant changes in concentrations of plasma glucose or insulin or in haemostatic factors.
Keywordscholesterol fat flaxseed oil glucose haemostasis hemp seed oil insulin
The authors wish to thank laboratory personnel, Ms. Erja Kinnunen, Mrs. Irja Kanniainen, and Mrs. Kaija Kettunen for excellent technical assistance. The study was financially supported by the National Technology Agency, Finland
- 1.Mensink RP, Katan MB (1992) Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler Thromb 12:911–919Google Scholar
- 2.Kris-Etherton PM, Krummel D, Russell ME, Dreon D, Mackey S, Borchers J, Wood PD (1988) The effect of diet on plasma lipids, lipoproteins, and coronary heart disease. J Am Diet Assoc 88:1373–1400Google Scholar
- 3.Hegsted DM, Ausman LM, Johnson JA, Dallal GE (1993) Dietary fat and serum lipids: an evaluation of the experimental data. Am J Clin Nutr 57:875–883Google Scholar
- 4.Harris WS (1997) N-3 fatty acids and serum lipoproteins: human studies. Am J Clin Nutr 65:1645S–1654SGoogle Scholar
- 7.Marckmann P, Sandström B, Jespersen J (1994) Low-fat, high-fiber diet favorably affects several independent risk markers of ischemic heart disease: observations on blood lipids, coagulation, and fibrinolysis from a trial of middle-aged Danes. Am J Clin Nutr 59:935–939Google Scholar
- 8.Marckmann P (1995) Diet, blood coagulation and fibrinolysis. Danish Med Bull 42:410–425Google Scholar
- 10.Rastas M, Seppänen R, Knuts L-R, Hakala P, Karttila V (1997) Nutrient composition of foods. The Social Insurance Institution, Turku, FinlandGoogle Scholar
- 11.Ågren JJ, Julkunen A, Penttilä I (1992) Rapid separation of serum lipids for analysis by a single aminopropyl column. J Lipid Res 33:1871–1876Google Scholar
- 12.Penttilä IM, Voutilainen E, Laitinen O, Juutilainen P (1981) Comparison of different analytical and precipitation methods for the direct estimation of high-density lipoprotein cholesterol. Scand J Lab Invest 41:353–360Google Scholar
- 13.Fruchart JC, Kora I, Cachera C, Clavey V, Duthilleul P, Moschetto Y (1982) Simultaneous measurement of plasma apolipoproteins A-I and B by electroimmunoassay. Clin Chem 28:59–62Google Scholar
- 14.Valsta LM, Salminen I, Aro A, Mutanen M (1996) Alpha-linolenic acid in rapeseed oil partly compensates for the effect of fish restriction on plasma long chain n-3 fatty acids. Eur J Clin Nutr 50:229–235Google Scholar
- 15.Mantzioris E, James MJ, Gibson RA, Cleland LG (1995) Differences exist in the relationships between dietary linoleic and alpha-linolenic acids and their respective long-chain metabolites. Am J Clin Nutr 61:320–324Google Scholar
- 16.Okuyama H, Kobayashi T, Watanabe (1997) Dietary fatty acids—the N-6/N-3 balance and chronic elderly diseases. Excess linoleic acid and relative N-3 deficiency syndrome seen in Japan. Prog Lipid Res 3:409–457Google Scholar
- 17.Brenner RR (1989) Factors influencing chain elongation and desaturation. In: Vergroesen AJ, Crawford M (eds) The role of fats in human nutrition, (2nd edn.). Academic Press Limited, London, pp 45–79Google Scholar
- 18.Simopoulos AP (1999) Essential fatty acids in health and chronic disease. Am J Clin Nutr 70:560S–569SGoogle Scholar
- 19.Callaway JC, Schwab U, Harvima I, Halonen P, Mykkänen O, Hyvönen P, Järvinen T (2005) Efficacy of dietary hempseed oil in patients with atopic dermatitis. J Derm Treat 16:87–94Google Scholar
- 23.Pawlosky RJ, Hibbeln JR, Novotny JA, Salem N Jr (2001) Physiological compartmental analysis of α-linolenic acid metabolism in adult humans. J Lipid Res 42:1257–1265Google Scholar
- 24.Fokkema MR, Brouwer DAJ, Hasperhoven MB, Martini IA, Muskiet FAJ (2000) Short-term supplementation of low-dose gamma-linolenic acid (GLA), alpha-linolenic acid (ALA), or GLA plus ALA does not augment LCP omega 3 status of Dutch vegans to an appreciable extent. Prostaglandins Leukot Essent Fatty Acids 63:287–292CrossRefGoogle Scholar
- 27.Kinosian B, Glick H, Preiss L, Puder KL (1995) Cholesterol and coronary heart disease: predicting risks in men by changes in levels and ratios. J Investig Med 43:443–450Google Scholar
- 28.Pang D, Allman-Farinelli MA, Wong T, Barnes R, Kingham KM (1998) Replacement of linoleic acid with alpha-linolenic acid does not alter blood lipids in normolipidaemic men. Br J Nutr 80:163–167Google Scholar
- 29.Bemelmans WJ, Broer J, Feskens EJ, Smit AJ, Muskiet FA, Lefrand JD, Bom VJ, May JF, Meyboom-de Jong B (2002) Effect of an increased intake of alpha-linolenic acid and group nutritional education on cardiovascular risk factors on the Mediterranean Alpha-linolenic Enriched Groningen Dietary Intervention (MARGARIN) study. Am J Clin Nutr 75:221–227Google Scholar
- 30.Zhao G, Etherton TD, Martin KR, West SG, Gillies PJ, Kris-Etherton PM (2004) Dietary α-linolenic acid reduces inflammatory and lipid cardiovascular risk factors in hypercholesterolemic men and women. J Nutr 134:2991–2997Google Scholar
- 32.Brouwer IA, Katan MB, Zock PL (2004) Dietary α-linolenic acid is associated with reduced risk of fatal coronary heart disease, but increased prostate cancer risk: a meta-analysis. J Nutr 134:919–922Google Scholar
- 33.Sanders TA, Oakely FR, Miller GJ, Mitropoulos KA, Crook D, Olive MF (1997) Influence of n-6 versus n-3 polyunsaturated fatty acids in diets low in saturated fatty acids on plasma lipoproteins and hemostatic factors. Arterioscler Thromb Vasc Biol 17:3449–3460Google Scholar
- 34.Finnegan YE, Howarth D, Minihane AM, Kew S, Miller GJ, Calder PC, Williams CM (2003) Plant and marine derived (n-3) polyunsaturated fatty acids do not affect blood coagulation and fibrinolytic factors in moderately hyperlipidemic humans. J Nutr 133:2210–2213Google Scholar
- 35.Freese R, Mutanen M (1997) Alpha-linolenic acid and marine long-chain n-3 fatty acids differ only slightly in their effects on hemostatic factors in healthy subjects. Am J Clin Nutr 66:591–598Google Scholar