Effects of a tryptic hydrolysate from bovine milk αS1–casein on hemodynamic responses in healthy human volunteers facing successive mental and physical stress situations
- 285 Downloads
Preclinical results in rats have demonstrated anxiolytic–like effects of a tryptic bovine αS1–casein hydrolysate.
Aim of the study
We investigated the putative effects of this tryptic hydrolysate on systolic (SBP), diastolic (DBP) blood pressures, heart rate (HR) values and plasma cortisol concentrations (CC) in human healthy volunteers facing successive stress situations.
The subjects were (double blind) randomly allocated to ingest three times, 12 hours apart, two capsules containing either 200 mg of αS1–casein hydrolysate (TS) or bovine skimmed milk powder as a placebo (CS). On the morning of the test day, a first blood sample for baseline measurement of CC was taken before the subjects were submitted to the Stroop test (ST) and, after a 30–min rest, to a Cold Pressor test (CPT). SBP, DBP, and HR were continuously recorded for 5 min before the ST and during each stress situation. A second blood sample was taken 15 min after the end of the CPT condition.
ST and ST + CPT combined test situations increased SBP, DBP and HR. The significant “Treatment × SBP” and “Treatment × DBP” interactions indicated the lower percentage changes in SBP and DBP of the TS. In addition, the results showed a significant decrease of the CC in the TS but not in the CS throughout the ST + CPT combined stress tests. HR remained stable in TS between the initial rest period and the CPT unlike what happened in CS.
On the basis of blood pressure and cortisol changes, these results suggest an antistress profile of this αS1–casein hydrolysate in human subjects.
Key wordsmilk αS1–casein hydrolysate blood pressure heart rate cortisol stress
Unable to display preview. Download preview PDF.
- 3.Chabance B, Jollès P, Izquierdo C, Mazoyer E, Francoual C, Drouet L, Fait AM (1995) Characterization of an antithrombotic peptide from k-casein in newborn plasma after milk ingestion. Br J Nutr 73:582–590Google Scholar
- 5.Léonil J, Bosc C, Maubois JL, Tomé D(2001) Protéines. In: Debry G, coordinateur, Tec et Doc (ed) Lait,Nutrition et Santé. Deuxième partie: Le lait et ses constituants: biodisponibilité et valeur nutritionnelle, pp 43–83Google Scholar
- 6.Maruyama S, Mitachi H, Awaya J, Kurono M, Tomizuka N, Susuki H (1987) Angiotensin-I converting enzyme inhibitory activity of the C-terminal hexapeptide of αs1-casein. Agric Biol Chem 51:2557–2561Google Scholar
- 7.Blass EM (1997) Infant formula quiets crying human newborns. J Dev Behav Pediatr 18:162–165Google Scholar
- 8.Lecouvey M, Frochot C, Miclo, Orlewski P, Driou A, Linden G, Gaillard JL, Marraud M, Cung MT and Vanderesse R (1997) Two-dimensional H-NMR and CD structural analysis in a micellar medium of a bovine αS1-casein fragment having benzodiazepine-like properties. Eur J Biochem 248:872–878CrossRefPubMedGoogle Scholar
- 10.Miclo L, Perrin E, Driou A, Papadopoulos V, Boujrad N, Vanderesse R, Boudier JF, Desor D, Linden G, Gaillard L (2001) Characterization of α-casozepine, a tryptic peptide from bovine α-casein with benzodiazepine-like activity. The FASEB Journal express article 10.1096/ fj 0.00–0685fje. Published online June 8:2001Google Scholar
- 14.Imholz BP (1996) Automated blood pressure measurement during ergometric stress testing: possibilities of Finapres. Z Kardiol 3:76–80Google Scholar
- 20.Weitzman ED, Fukushima D, Nogeire C, Roffwarg H, Gallagher TF, Hellman L (1971) Twenty four hour pattern of the episodic secretion of cortisol in normal subjects. J Clin Endocr Met 33:13–22Google Scholar