European Journal of Nutrition

, Volume 44, Issue 2, pp 128–132 | Cite as

Effects of a tryptic hydrolysate from bovine milk αS1–casein on hemodynamic responses in healthy human volunteers facing successive mental and physical stress situations

  • M. MessaoudiEmail author
  • C. Lefranc–Millot
  • D. Desor
  • B. Demagny
  • L. Bourdon



Preclinical results in rats have demonstrated anxiolytic–like effects of a tryptic bovine αS1–casein hydrolysate.

Aim of the study

We investigated the putative effects of this tryptic hydrolysate on systolic (SBP), diastolic (DBP) blood pressures, heart rate (HR) values and plasma cortisol concentrations (CC) in human healthy volunteers facing successive stress situations.


The subjects were (double blind) randomly allocated to ingest three times, 12 hours apart, two capsules containing either 200 mg of αS1–casein hydrolysate (TS) or bovine skimmed milk powder as a placebo (CS). On the morning of the test day, a first blood sample for baseline measurement of CC was taken before the subjects were submitted to the Stroop test (ST) and, after a 30–min rest, to a Cold Pressor test (CPT). SBP, DBP, and HR were continuously recorded for 5 min before the ST and during each stress situation. A second blood sample was taken 15 min after the end of the CPT condition.


ST and ST + CPT combined test situations increased SBP, DBP and HR. The significant “Treatment × SBP” and “Treatment × DBP” interactions indicated the lower percentage changes in SBP and DBP of the TS. In addition, the results showed a significant decrease of the CC in the TS but not in the CS throughout the ST + CPT combined stress tests. HR remained stable in TS between the initial rest period and the CPT unlike what happened in CS.


On the basis of blood pressure and cortisol changes, these results suggest an antistress profile of this αS1–casein hydrolysate in human subjects.

Key words

milk αS1–casein hydrolysate blood pressure heart rate cortisol stress 


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  1. 1.
    Meisel H (1997) Biochemical properties of regulatory peptides derived from milk proteins. Biopolymers 43:119–128CrossRefPubMedGoogle Scholar
  2. 2.
    Clare DA, Swaisgood HE (2000) Bioactive milk peptides: a prospectus. J Dairy Sci 83:1187–1195PubMedGoogle Scholar
  3. 3.
    Chabance B, Jollès P, Izquierdo C, Mazoyer E, Francoual C, Drouet L, Fait AM (1995) Characterization of an antithrombotic peptide from k-casein in newborn plasma after milk ingestion. Br J Nutr 73:582–590Google Scholar
  4. 4.
    Meisel H, FitzGerald RJ (2000) Opioid peptides encrypted in intact milk protein sequences. BR J Nutr 84(Animal):S27–S31PubMedGoogle Scholar
  5. 5.
    Léonil J, Bosc C, Maubois JL, Tomé D(2001) Protéines. In: Debry G, coordinateur, Tec et Doc (ed) Lait,Nutrition et Santé. Deuxième partie: Le lait et ses constituants: biodisponibilité et valeur nutritionnelle, pp 43–83Google Scholar
  6. 6.
    Maruyama S, Mitachi H, Awaya J, Kurono M, Tomizuka N, Susuki H (1987) Angiotensin-I converting enzyme inhibitory activity of the C-terminal hexapeptide of αs1-casein. Agric Biol Chem 51:2557–2561Google Scholar
  7. 7.
    Blass EM (1997) Infant formula quiets crying human newborns. J Dev Behav Pediatr 18:162–165Google Scholar
  8. 8.
    Lecouvey M, Frochot C, Miclo, Orlewski P, Driou A, Linden G, Gaillard JL, Marraud M, Cung MT and Vanderesse R (1997) Two-dimensional H-NMR and CD structural analysis in a micellar medium of a bovine αS1-casein fragment having benzodiazepine-like properties. Eur J Biochem 248:872–878CrossRefPubMedGoogle Scholar
  9. 9.
    Lecouvey M, Frochot C, Miclo L, Orlewski P, Marraud M, Gaillard JL, Thon Cung M, Vanderesse R (1997) Conformational studies of a benzodiazepinlike peptide in SDS micelles by circular dichroism, H NMR and molecular dynamics simulation. Lett Pept Sci 4:359–364CrossRefGoogle Scholar
  10. 10.
    Miclo L, Perrin E, Driou A, Papadopoulos V, Boujrad N, Vanderesse R, Boudier JF, Desor D, Linden G, Gaillard L (2001) Characterization of α-casozepine, a tryptic peptide from bovine α-casein with benzodiazepine-like activity. The FASEB Journal express article 10.1096/ fj 0.00–0685fje. Published online June 8:2001Google Scholar
  11. 11.
    Tulen JH, Moleman P, van Steenis HG, Boosmsma F (1989) Characterization of stress reactions to the Stroop Color Word Test. Pharmacol Biochem Behav 32:9–15CrossRefPubMedGoogle Scholar
  12. 12.
    Fasano ML, Sand T, Brubakk AO, Kruszewski P, Bordini C, Sjaastad O (1996) Reproducibility of the cold pressor test: studies in normal subjects. Clin Auton Res 6:249–253PubMedGoogle Scholar
  13. 13.
    Bohnen N, Nicolson N, Sulon J, Jolles J (1991) Coping style, trait anxiety and cortisol reactivity during mental stress. J Psychosom Res 35:141–147CrossRefPubMedGoogle Scholar
  14. 14.
    Imholz BP (1996) Automated blood pressure measurement during ergometric stress testing: possibilities of Finapres. Z Kardiol 3:76–80Google Scholar
  15. 15.
    Allen MT, Obrist PA, Sherwood A, Crowell MD (1987) Evaluation of myocardial and peripheral vascular responses during reaction time, mental arithmetic and cold pressor tasks. Psychophysiology 24:648–656PubMedGoogle Scholar
  16. 16.
    Hoshikawa Y, Yamamoto Y (1997) Effects of Stroop color-word conflict test on the autonomic system responses. Am J Physiol 272(Pt2):H1113–H1121PubMedGoogle Scholar
  17. 17.
    Seals DR (1990) Sympathetic activation during the cold pressure test: influence of stimulus area. Clin Physiol 10:123–129PubMedGoogle Scholar
  18. 18.
    Belkic KL, Mickovic L, Milic B, Savic S (1987) Blood pressure and electrocardiographic changes elicited by the glare pressor test. Arch Environ Health 42:37–43PubMedGoogle Scholar
  19. 19.
    Negrao AB, Deuster PA, Gold PW, Singh A, Chrousos GP (2000) Individual reactivity and physiology of the stress response. Biomed Pharmacother 54:122–128CrossRefPubMedGoogle Scholar
  20. 20.
    Weitzman ED, Fukushima D, Nogeire C, Roffwarg H, Gallagher TF, Hellman L (1971) Twenty four hour pattern of the episodic secretion of cortisol in normal subjects. J Clin Endocr Met 33:13–22Google Scholar

Copyright information

© Steinkopff Verlag 2004

Authors and Affiliations

  • M. Messaoudi
    • 1
    Email author
  • C. Lefranc–Millot
    • 2
  • D. Desor
    • 3
  • B. Demagny
    • 2
  • L. Bourdon
    • 4
  1. 1.ETAP-Applied Ethology 13Vandoeuvre-lès-NancyFrance
  2. 2.INGREDIA ArrasFrance
  3. 3.Laboratoire de Neurosciences ComportementalesFaculté des Sciences Université Henri PoincaréVandoeuvre-lès-NancyFrance
  4. 4.Centre de Recherches du Service de Santé des ArméesLa TroncheFrance

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