Early outgrowth EPCs generation is reduced in patients with Buerger’s disease
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Buerger’s disease often shows poor collateral artery generation (i.e. neovascularization) in the ischemic limbs. However, the etiology has not yet been clarified. Circulating endothelial progenitor cells (EPCs) derived from bone marrow contribute to neovascularization in the multi-step process which includes the following capacities; mobilization, differentiation, adhesion, migration, invasion and secretion.
Materials and methods
We assessed EPCs capacities in vitro and ex vivo in age- and sex-matched controls (n = 12) and patients with Buerger’s disease (n = 12), derived from peripheral blood-derived mononuclear cells (PB-MNCs).
In the flow cytometry analysis, the numbers of circulating EPC (CD34+/KDR+ or CD133+/KDR+ PB-MNC) were similar between controls and patients with Buerger’s disease. Next, we cultured PB-MNC to obtain EPCs. The number of early outgrowth EPCs was significantly decreased in patients with Buerger’s disease (p < 0.005), indicating the reduced generation of early outgrowth EPCs in Buerger’s disease. However, adhesion, migration, invasion and secretion capacities were not impaired in patients with Buerger’s disease.
The early outgrowth EPCs generation is reduced in patients with Buerger’s disease.
KeywordsBuerger’s disease Thromboangiitis obliterans Endothelial progenitor cell Cell function
Endothelial progenitor cells
Peripheral blood-derived mononuclear cells
Kinase domain receptor
Vascular endothelial growth factor
Fluorescence-activated cell sorting
Human umbilical vein endothelial cell
1,1′-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labeled acetylated low-density lipoprotein
Basic fibroblast growth factor
Platelet-derived growth factor BB
Insulin growth factor
Stromal cell-derived factor-1α
Enzyme-linked immunosorbent assay
- IL-1, -6, -8
Interleukin-1, -6, -8
Tumor growth factor-1α
Vascular cell adhesion molecule-1
Monocyte chemotactic protein-1
High-sensitivity C-reactive protein
Messenger ribonucleic acid
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