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International Journal of Colorectal Disease

, Volume 34, Issue 1, pp 193–196 | Cite as

Obstructed defecation—an enteric neuropathy? An exploratory study of patient samples

  • Mia KimEmail author
  • Corinna Rosenbaum
  • Nicolas Schlegel
  • Christian Grumaz
  • Kai Sohn
  • Christoph Isbert
  • Heike Walles
  • Marco Metzger
Short Communication

Abstract

Purpose

Although various strategies exist for chronic constipation therapy, the pathogenesis of chronic constipation is still not completely understood. The aim of this exploratory experimental study is to elucidate alterations of the autonomous enteric nervous system at the molecular level in patients with obstructed defecation, who represent one of the most predominant groups of constipated patients.

Methods

Full-thickness rectal wall samples of patients with obstructed defecation were analyzed and compared with controls. Differential gene expression analyses by RNA-Seq transcriptome profiling were performed and gene expression profiles were assigned to gene ontology pathways by application of different biological libraries.

Results

Analysis of the transcriptome showed that genes associated with the enteric nervous system functions were significantly downregulated in patients with obstructed defecation. These affected functions included developmental processes and synaptic transmission.

Conclusions

Our results therefore indicate that obstructed defecation may represent an enteric neuropathy, comparable to Hirschsprung disease and slow-transit constipation.

Keywords

Chronic constipation Obstructed defecation Enteric nervous system Enteric neuropathy Transcriptome profiling, RNA-Seq 

Notes

Acknowledgements

We would like to thank Mrs. Renate Bausch, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Stuttgart, Germany, for technical assistance.

Funding

This project was funded by the Interdisciplinary Center of Clinical Studies (IZKF) Würzburg (Z-2/46).

Compliance with ethical standards

The study was approved by an institutional review board (Ethics committee, University Würzburg, Germany, approval number 23/12). All patients gave their informed, written consent.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

384_2018_3160_MOESM1_ESM.xlsx (10.7 mb)
ESM 1 (XLSX 10917 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.General, Visceral, Transplantational, Vascular and Paedeatric SurgeryUniversity Hospital WürzburgWürzburgGermany
  2. 2.Department for Tissue Engineering and Regenerative MedicineUniversity Hospital WürzburgWürzburgGermany
  3. 3.Department of Molecular BiotechnologyFraunhofer Institute for Interfacial Engineering and Biotechnology (IGB)StuttgartGermany
  4. 4.Amalie Sieveking HospitalHamburgGermany
  5. 5.Translational Centre TZKMEFraunhofer-Institute Interfacial Engineering and Biotechnology IGBWürzburgGermany

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