Coadministration of basic fibroblast growth factor-loaded polycaprolactone beads and autologous myoblasts in a dog model of fecal incontinence
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Basic fibroblastic growth factor (bFGF), a member of the heparin-binding growth factor family, regulates muscle differentiation. We investigated whether coadministration of autologous myoblasts and bFGF-loaded polycaprolactone beads could improve sphincter recovery in a dog model of fecal incontinence (FI).
FI was induced by resecting 25 % of the posterior anal sphincter in ten mongrel dogs. One month later, the dogs were randomized to receive either PKH-26-labeled autologous myoblasts alone (M group, five dogs) or autologous myoblasts and bFGF-loaded polycaprolactone beads (MBG group, five dogs). The outcomes included anal manometry, compound muscle action potentials (CMAPs) of the pudendal nerve, and histology.
The increase in anal contractile pressure over 3 months was significantly greater in the MBG group (from 4.85 to 6.83 mmHg) than that in the M group (from 4.94 to 4.25 mmHg), with a coefficient for the difference in recovery rate of 2.672 (95 % confidence interval [CI] 0.962 to 4.373, p = 0.002). The change in the CMAP amplitude was also significantly greater in the MBG group (from 0.59 to 1.56 mV) than that in the M group (from 0.81 to 0.67 mV) (coefficient 1.114, 95 % CI 0.43 to 1.80, p = 0.001). Labeled cells were detected in 2/5 (40 %) and 5/5 (100 %) dogs in the M and MBG groups, respectively.
Coadministration of bFGF-loaded PCL beads and autologous myoblasts improved the recovery of sphincter function in a dog model of FI and had better outcomes than cell-based therapy alone.
KeywordsFecal incontinence Tissue engineering Myoblast Tissue regeneration Growth factor
The authors thank Myong Hoon Ihn, Jung-Woo Park, Ok-Sun Kim, Sang A Lee, Tae-Ho Kim, Jun-Gu Kang, and Hye-Jin Kim for their assistance and advice.
Conflicts of interest
The authors have no conflicts of interest to report. This work was supported by a grant from the Korean Health Technology R&D Project, The Ministry of Health and Welfare, Republic of Korea (Grant No. A120357).
Research involving human participants and/or snimals (ethical approval)
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the the Animal Care and Use Committee of Seoul National University Bundang Hospital (64-2012-083).
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