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L-arginine and glycine supplementation in the repair of the irradiated colonic wall of rats

  • Etiene de Aguiar Picanço
  • Francisco Lopes-Paulo
  • Ruy G. Marques
  • Cristina F. Diestel
  • Carlos Eduardo R. Caetano
  • Mônica Vieira Mano de Souza
  • Gabriela Mendes Moscoso
  • Helena Maria F. Pazos
Original Article

Abstract

Purpose

Radiotherapy is widely used for cancer treatment but has harmful effects. This study aimed to assess the effects of L-arginine and glycine supplementation on the colon wall of rats submitted to abdominal irradiation.

Methods

Forty male Wistar rats were randomly divided into four groups: I—healthy, II—irradiated with no amino acid supplementation, III—irradiated and supplemented with L-arginine, and IV—irradiated and supplemented with glycine. The animals received supplementation for 14 days, with irradiation being applied on the eighth day of the experiment. All animals underwent laparotomy on the 15th day for resection of a colonic segment for stereologic analysis. Parametric and nonparametric tests were used for statistical analysis, with the level of significance set at p ≤0.05.

Results

Stereologic analysis showed that irradiation induced a reduction of the total volume of the colon wall of group II and III animals compared to healthy controls, but not of group IV animals supplemented with glycine. The mucosal layer of the irradiated animals of all groups was reduced compared to healthy group I animals, but supplementation with L-arginine and glycine was effective in maintaining the epithelial surface of the mucosal layer.

Conclusion

The present results suggest that glycine supplementation had a superior effect on the irradiated colon wall compared to L-arginine supplementation since it was able to maintain the thickness of the wall and the epithelial surface of the mucosa, whereas L-arginine maintained the partial volume of the epithelium and the epithelial surface, but not the total volume of the intestinal wall.

Keywords

L-arginine Glycine Irradiation Colitis Nutrition Radiotherapy 

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Etiene de Aguiar Picanço
    • 1
  • Francisco Lopes-Paulo
    • 2
  • Ruy G. Marques
    • 2
  • Cristina F. Diestel
    • 3
  • Carlos Eduardo R. Caetano
    • 1
  • Mônica Vieira Mano de Souza
    • 1
  • Gabriela Mendes Moscoso
    • 1
  • Helena Maria F. Pazos
    • 4
  1. 1.Laboratory of Experimental SurgeryRio de Janeiro State UniversityRio de JaneiroBrazil
  2. 2.Department of General SurgeryRio de Janeiro State UniversityRio de JaneiroBrazil
  3. 3.Nutrition Division, Pedro Ernesto University HospitalRio de Janeiro State UniversityRio de JaneiroBrazil
  4. 4.Department of Urogenital AnatomyRio de Janeiro State UniversityRio de JaneiroBrazil

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