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International Journal of Colorectal Disease

, Volume 24, Issue 12, pp 1389–1394 | Cite as

Haemorrhoids are related to changes of cell function in mucosa and submucosa

  • Christian Klink
  • Marcel Binnebösel
  • Daniel Kämmer
  • Stefan Willis
  • Andreas Prescher
  • Uwe Klinge
  • Volker Schumpelick
Original Article

Abstract

Purpose

Epidemiology and risk factors of haemorrhoidal disease are not well defined. This study tried to evaluate if the appearance of haemorrhoids is related to a disturbed remodelling of the soft tissue of rectal mucosa and submucosa. Therefore, immunohistochemical expression profiles of five parameters as potential mediators in neoangiogenesis (EGFR), in inflammatory cell activity (COX-2), and in cell migration, differentiation, and wound healing (notch-3, c-myc, and ß-Catenin) were analysed (Saed et al., Fertil Steril 83(Suppl 1):1216–1219, 1; Saed et al., Fertil Steril 79:1404–1408, 2; Stojadinovic et al., Am J Pathol 167:59–69, 3).

Methods

Haemorrhoidal tissue specimens were collected from 44 patients. Healthy rectal mucosa was obtained from 16 non-fixed fresh cadavers and served as control. Histological and immunohistochemical markers like EGFR, COX-2, notch-3, c-myc, and ß-Catenin were analysed semi-quantitatively, separately for mucosal and submucosal layer.

Results

Significantly increased expressions were found for EGFR, COX-2, and notch-3 in the mucosal and submucosal layer of haemorrhoidal tissue in comparison to normal rectal tissue.

Conclusions

This finding confirms that haemorrhoidal disease may be regarded as a manifestation of a soft tissue disease.

Keywords

Haemorrhoids Extracellular matrix Pathogenesis Wound healing 

Notes

Acknowledgments

We are grateful to Mrs. Ellen Krott for most excellent and careful assistance during this investigation.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Christian Klink
    • 1
  • Marcel Binnebösel
    • 1
  • Daniel Kämmer
    • 1
  • Stefan Willis
    • 2
  • Andreas Prescher
    • 3
  • Uwe Klinge
    • 1
    • 4
  • Volker Schumpelick
    • 1
  1. 1.Department of SurgeryRWTH Aachen University HospitalAachenGermany
  2. 2.Department of SurgeryKlinikum Ludwigshafen/RheinLudwigshafenGermany
  3. 3.Institute of Anatomy and Cell Biology, RWTH AachenAachenGermany
  4. 4.Applied Medical EngineeringHelmholtz Institut, RWTH AachenAachenGermany

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