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International Journal of Colorectal Disease

, Volume 23, Issue 1, pp 29–35 | Cite as

Genetic alterations of APC, K-ras, p53, MSI, and MAGE in Korean colorectal cancer patients

  • Chang-Ho JeonEmail author
  • Han-IL Lee
  • Im-Hee Shin
  • Jong-Wook Park
Original Article

Abstract

Background and aim

Colorectal cancer (CRC) is one of the most rapidly increasing cancers in Korea, but no comprehensive analysis has been performed to speculate the genetic basis of CRC development. We investigated the presence of adenomatous polyposis coli gene (APC), Kirsten-ras (K-ras), p53, microsatellite instability (MSI), and melanoma antigen gene (MAGE) alterations in CRC and correlated the results obtained with clinical data.

Materials and methods

We collected 78 cancer tissues from CRC patients. Genetic analyses were performed on APC, K-ras, p53, and MSI (BAT 25 and BAT 26), and in addition, MAGE expression was tested by reverse transcription polymerase chain reaction. Correlations between genetic markers and clinical factors were analyzed after reviewing medical records.

Result

The positive rates for alterations of APC, K-ras, p53, MSI, and MAGE in 78 tissue samples were 33.3, 29.5, 34.6, 9.0, and 68.4%, respectively. Mutations were frequently detected in codons 1291 and 1450 of APC, in codon 12 of K-ras and in codons 248, 282, and 176 of p53. APC mutations were frequently noted in early-stage cancer, whereas MSI was observed in right-sided and multiple cancers. No associations were found between the presence of alterations in APC, K-ras, p53, MSI, and MAGE.

Interpretation

In Koreans, positive rates of alterations in APC and p53 were slightly lower than those of APC and p53 in Caucasians, and the genetic alterations including MAGE expression are involved in 92.1% of CRCs. The lack of multiple mutations and of a relation between mutation rates and clinical stage suggest that genetic alterations might have independent influences on CRC development in Koreans.

Keywords

Colorectal neoplasm APC K-ras p53 MAGE 

Notes

Acknowledgments

This work was equally supported by two research funds. (The Korean Institute of Industrial Technology Evaluation and Planning through the Biomolecular Engineering Center at Kyungpook National University and grant no. RTI04-03-01 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy).

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Chang-Ho Jeon
    • 1
    Email author
  • Han-IL Lee
    • 2
  • Im-Hee Shin
    • 3
  • Jong-Wook Park
    • 4
  1. 1.Department of Laboratory Medicine, School of MedicineCatholic University of DaeguDaeguSouth Korea
  2. 2.Department of Surgery, School of MedicineCatholic University of DaeguDaeguSouth Korea
  3. 3.Department of Medical Statistics, School of MedicineCatholic University of DaeguDaeguSouth Korea
  4. 4.Department of Immunology, School of MedicineKeimyung UniversityKeimyungSouth Korea

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