International Journal of Colorectal Disease

, Volume 20, Issue 1, pp 9–17

Ameliorative effect of IDS 30, a stinging nettle leaf extract, on chronic colitis

  • Astrid Konrad
  • Michael Mähler
  • Stephan Arni
  • Beatrice Flogerzi
  • Sonja Klingelhöfer
  • Frank Seibold
Original Article

DOI: 10.1007/s00384-004-0619-z

Cite this article as:
Konrad, A., Mähler, M., Arni, S. et al. Int J Colorectal Dis (2005) 20: 9. doi:10.1007/s00384-004-0619-z

Abstract

Background and aims

Anti-TNF-α antibodies are very effective in the treatment of acute Crohn’s disease, but are limited by the decline of their effectiveness after repeated applications. The stinging nettle leaf extract, IDS 30, is an adjuvant remedy in rheumatic diseases dependent on a cytokine suppressive effect. We investigated the effect of IDS 30 on disease activity of murine colitis in different models.

Methods

C3H.IL-10−/− and BALB/c mice with colitis induced by dextran sodium sulphate (DSS) were treated with either IDS 30 or water. Mice were monitored for clinical signs of colitis. Inflammation was scored histologically, and faecal IL-1β and mucosal cytokines were measured by ELISA. Mononuclear cell proliferation of spleen and Peyer’s patches were quantified by 3H-thymidine.

Results

Mice with chronic DSS colitis or IL-10−/− mice treated with IDS 30 clinically and histologically revealed significantly (p<0.05) fewer signs of colitis than untreated animals. Furthermore, faecal IL-1β and mucosal TNF-α concentrations were significantly lower (p<0.05) in treated mice. Mononuclear cell proliferation after stimulation with lipopolysaccharide was significantly (p<0.001) reduced in mice treated with IDS 30.

Conclusions

The long-term use of IDS 30 is effective in the prevention of chronic murine colitis. This effect seems to be due to a decrease in the Th1 response and may be a new therapeutic option for prolonging remission in inflammatory bowel disease.

Keywords

Inflammatory bowel disease IDS 30 Murine colitis TNF-α 

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Astrid Konrad
    • 1
  • Michael Mähler
    • 2
  • Stephan Arni
    • 1
  • Beatrice Flogerzi
    • 1
  • Sonja Klingelhöfer
    • 3
  • Frank Seibold
    • 1
  1. 1.Division of Gastroenterology, Inselspital, University HospitalUniversity of BernBerneSwitzerland
  2. 2.Central Animal Facility and Institute for Laboratory Animal ScienceMedical School HannoverHannoverGermany
  3. 3.Strathmann AGHamburgGermany

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