Protective effects of vitamin D against injury in intestinal epithelium
Vitamin D deficiency is associated with intestinal barrier dysfunction, which contributes to pathogenesis of acute intestinal injury in children. We aim to investigate the effects of vitamin D on intestinal injury in intestinal epithelial cells and organoids.
Lipopolysaccharide (LPS) was used to induce injury in intestinal epithelial cells (IEC-18) and organoids, and the effect of vitamin D was assessed. Cell viability was measured and inflammation cytokines TNFα and IL-8 were quantified. FITC-dextran 4 kDa (FD4) permeability was measured using Transwell while tight junction markers were assessed by immunofluorescence staining in IEC-18 and intestinal organoids. Data were compared using one-way ANOVA with Bonferroni post-test.
IEC-18 viability was decreased by LPS treatment, but was prevented by vitamin D. The upregulation of inflammation was inhibited by vitamin D, which also decreased epithelium permeability. Vitamin D restored tight junction ZO-1 and claudin 2. In addition, vitamin D decreased TNFα expression and prevented the disruption of ZO-1 in injured organoids.
Vitamin D rescued epithelial barrier function by improving permeability and restoring tight junctions, leading to decrease inflammation. This study confirms the protective effects of vitamin D, which could be used as a treatment strategy for infants at risk of developing intestinal injury.
KeywordsVitamin D Intestinal epithelial cells Permeability Organoids
This work is supported by a Canadian Institutes of Health Research (CIHR) Foundation Grant 353857. AP is the Robert M. Filler Chair of Surgery, The Hospital for Sick Children (HSC).
CL: Conception and design, Collection and/or assembly of data, Data analysis and interpretation, Manuscript writing, Final approval of manuscript. EL, SC, RF, BL, HZ, MY: Collection and/or assembly of data, Data analysis and interpretation, Final approval of manuscript. AP: Conception and design, Financial support, Final approval of manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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