Pediatric Surgery International

, Volume 27, Issue 8, pp 857–862 | Cite as

Ischemic preconditioning and remote ischemic preconditioning have protective effect against cold ischemia–reperfusion injury of rat small intestine

  • Isamu Saeki
  • Toshiharu Matsuura
  • Makoto Hayashida
  • Tomoaki Taguchi
Original Article



To investigate the protective effect of ischemic preconditioning (IPC) and remote ischemic preconditioning (RIPC) against cold ischemia–reperfusion injury (IRI) associated with small bowel transplantation (SBT).


Male Lewis rats weighing 200–300 g were used for this study. The rats were assigned to three groups: control, ischemic preconditioning (IPC), or remote ischemic preconditioning (RIPC). Heterotopic SBT was thereafter performed. The recipient rats were killed 3, 6, 12 and 24 h after transplantation. Specimens from the intestine were histologically scored according to a grading system (Park et al.). Serum lactate dehydrogenase (LDH), aspirate aminotransferase (AST), alanine aminotransferase (ALT) were examined and heme oxygenase-1 (HO-1) were analyzed by ELISA where HO-1 served as an indicator of protection against IRI.


The values of tissue injury were significantly lower in the IPC and RIPC groups than in control group at 3 h after SBT. The serum LDH, AST and ALT levels also significantly decreased in the IPC and RIPC groups at 3 h after SBT, but these protective effects against cold IRI diminished by 12 and 24 h after SBT. The serum HO-1 level significantly increased in the IPC and RIPC groups 3 h after SBT.


Both IPC and RIPC were found to ameliorate ischemia–reperfusion injury after rat SBT in the early phase. HO-1 may therefore play a protective role against cold IRI.


Ischemic preconditioning Remote ischemic preconditioning Intestine Small bowel transplantation Ischemia–reperfusion injury HO-1 



Ischemic preconditioning


Remote ischemic preconditioning


Small bowel transplantation


Ischemia–reperfusion injury


Heme oxygenase-1



This work was supported by a Grant-in-Aid for Young Scientists (Start-up 20890158). The authors also thank Brian Quinn for reviewing the manuscript.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Isamu Saeki
    • 1
  • Toshiharu Matsuura
    • 1
  • Makoto Hayashida
    • 1
  • Tomoaki Taguchi
    • 1
  1. 1.Kyushu UniversityFukuokaJapan

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