Amniotic fluid stem cell migration after intraperitoneal injection in pup rats: implication for therapy
- First Online:
- 304 Downloads
Despite being commonly used in clinical practice, the intraperitoneal (i.p.) route has been rarely used for cell delivery. We evaluated the capacity of amniotic fluid stem (AFS) cells, administered i.p., to diffuse systemically and to integrate into tissues of healthy newborn rats.
AFS cells were obtained from pregnant GFP + Sprague–Dawley rats by c-kit selection. Wild-type Sprague–Dawley newborn rats were divided into two groups receiving i.p.: (1) 2 × 106 AFS cells (n = 12); (2) of phosphate buffer saline (PBS) (n = 2) at 24 and 48 h after birth. Animals were either killed at 96 h of life, and organs collected for gfp amplification, or at 3 weeks of life and tissues isolated for green fluorescence protein (GFP) immunofluorescence.
No adverse effects were observed after i.p. injection of PBS or AFS cells. Gfp was amplified in at least one organ in all rats injected with AFS cells except one (11/12). The intestine was the organ found most frequently positive (67%) followed by liver (25%), spleen (16%), heart (16%), lungs (16%), femur (8%) and brain (0%). Immunohistochemistry confirmed PCR results.
In the short term, the i.p. administration of AFS cells, is a safe procedure and allows their migration, homing and integration into various organs of healthy newborn rats.
KeywordsPup rat Amniotic fluid stem cell Intraperitoneal injection Cell therapy
- 8.Chua TC, Yan TD, Saxena A, Morris DL (2009) Should the treatment of peritoneal carcinomatosis by cytoreductive surgery and hyperthermic intraperitoneal chemotherapy still be regarded as a highly morbid procedure: a systematic review of morbidity and mortality. Ann Surg 249(6):900–907CrossRefPubMedGoogle Scholar
- 9.Kumagai K, Saikawa Y, Fukuda K, Ito R, Igarashi T, Tsuwano S, Nakamura R, Takahashi T, Takeuchi H, Kitagawa Y (2009) Octreotide acetate successfully treated a bowel obstruction caused by peritoneally disseminated gastric cancer, there by enabling the subsequent use of oral S-1 chemotherapy. Int J Clin Oncol 14(4):372–375CrossRefPubMedGoogle Scholar
- 11.Chua TC, Yan TD, Zhao J, Morris DL (2009) Peritoneal carcinomatosis and liver metastases from colorectal cancer treated with cytoreductive surgery perioperative intraperitoneal chemotherapy and liver resection. Eur J Surg Oncol 2009 July 23Google Scholar
- 15.Di Campli C, Piscaglia AC, Pierelli L, Rutella S, Bonanno G, Alison MR, Mariotti A, Vecchio FM, Nestola M, Monego G, Michetti F, Mancuso S, Pola P, Leone G, Gasbarrini G, Gasbarrini A (2004) A human umbilical cord stem cell rescue therapy in a murine model of toxic liver injury. Dig Liver Dis 36(9):603–613CrossRefPubMedGoogle Scholar
- 16.Piscaglia AC, Di Campli C, Zocco MA, Di Gioacchino G, Novi M, Rutella S, Bonanno G, Monego G, Vecchio FM, Michetti F, Mancuso S, Leone G, Gasbarrini G, Pola P, Gasbarrini A (2005) Human cordonal stem cell intraperitoneal injection can represent a rescue therapy after an acute hepatic damage in immunocompetent rats. Transplant Proc 37(6):2711–2714CrossRefPubMedGoogle Scholar
- 22.Caughey AB, Hopkins LM, Norton ME (2006) Chorionic villus sampling compared with amniocentesis and the difference in the rate of pregnancy loss. Obstet Gynecol 108(3 Pt 1):612–616Google Scholar
- 23.Eddleman KA, Malone FD, Sullivan L, Dukes K, Berkowitz RL, Kharbutli Y, Porter TF, Luthy DA, Comstock CH, Saade GR, Klugman S, Dugoff L, Craigo SD, Timor-Tritsch IE, Carr SR, Wolfe HM, D’Alton ME (2006) Pregnancy loss rates after midtrimester amniocentesis. Obstet Gynecol 108(5):1067–1072PubMedGoogle Scholar
- 25.Carraro G, Perin L, Sedrakyan S, Giuliani S, Tiozzo C, Lee J, Turcatel G, De Langhe SP, Driscoll B, Bellusci S, Minoo P, Atala A, De Filippo RE, Warburton D (2008) Human amniotic fluid stem cells can integrate and differentiate into epithelial lung lineages. Stem Cells 26(11):2902–2911CrossRefPubMedGoogle Scholar
- 32.Hristov M, Weber C (2009) Progenitor cell trafficking in the vascular wall. J Thromb Haemost Suppl 1:31–34Google Scholar
- 35.Lundberg J, Le Blanc K, Söderman M, Andersson T, Holmin S (2009) Endovascular transplantation of stem cells to the injured rat CNS. Neuroradiology June 27Google Scholar
- 38.Chiavegato A, Bollini S, Pozzobon M, Callegari A, Gasparotto L, Taiani J, Piccoli M, Lenzini E, Gerosa G, Vendramin I, Cozzi E, Angelini A, Iop L, Zanon GF, Atala A, De Coppi P, Sartore S (2007) Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat. J Mol Cell Cardiol 42(4):746–759CrossRefPubMedGoogle Scholar