Advertisement

Pediatric Surgery International

, Volume 20, Issue 3, pp 185–191 | Cite as

Effect of dietary fat on fat absorption and concomitant plasma and tissue fat composition in a rat model of short bowel syndrome

  • I. Sukhotnik
  • N. Mor-Vaknin
  • R. A. Drongowski
  • A. G. Coran
  • C. M. Harmon
Original Article

Abstract

The aim of this study was to investigate the effect of dietary fat on the time course of changes in fat absorption and tissue and plasma lipid composition in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats underwent either a bowel transection with re-anastomosis (Sham rats) or 75% small bowel resection (SBS rats). Animals were randomly assigned to one of three groups: Sham rats fed normal chow (Sham-NC), SBS rats fed normal chow (SBS-NC), or SBS rats fed a high-fat diet (SBS-HFD). Rats were sacrificed on day 3 or 14. Body weight, food intake, food clearance (dry fecal mass), and fat clearance (total fecal fat) were measured twice a week. Fat and energy intakes were calculated according to the amount of ingested food. Food and fat absorbability were calculated as intake minus clearance and were expressed as percent of intake. Serum cholesterol, triglyceride, and albumin were measured. Total lipid composition of the liver, epididymal adipose tissue, and the small intestine was determined. Statistical analysis was performed by a Student’s test, with p values <0.05 considered significant. Both food and fat absorbability diminished after bowel resection in rats fed NC. This was accompanied by a decrease in body weight gain, plasma triglyceride and protein levels, and total lipid content of the liver at day 3 and of a decrease in adipose tissue at day 14 following operation. SBS-HFD rats experienced a significant increase ( p <0.05) in food absorbability after 7 days and fat absorbability after 3 days compared with Sham-NC and SBS-NC rats ( p <0.05), as well as increases in serum cholesterol, triglycerides, and glucose compared with SBS-NC rats. On day 14, plasma lipid levels in SBS-HFD rats were not different from SBS-NC or control rats; however, albumin levels were higher. A high-fat diet increased total fat content of the liver early after operation. In conclusion, in a rat model of SBS, an early high-fat diet increased the absorptive capacity of the intestinal remnant as seen by increased food and fat absorbability. These findings suggest a benefit of a high-fat diet on intestinal adaptation in general and on lipid absorption in particular.

Keywords

Short bowel syndrome Dietary lipid Fat absorption Tissue lipids Plasma lipids 

References

  1. 1.
    Taylor SF, Sokol RJ (1995) Infants with short bowel syndrome. In: Hay WW (ed) Neonatal nutrition and metabolism. Mosby, St. Louis, pp 432–450Google Scholar
  2. 2.
    Biller JA (1987) Short bowel syndrome. In: Grand RI, Sutphen JL, Dietz WH (eds) Pediatric nutrition. Theory and practice. Butterworth, Stoneham, MA, pp 481–487Google Scholar
  3. 3.
    Vanderhoof JA (1996) Short bowel syndrome. Neonatal Gastroenterol 23:377–386Google Scholar
  4. 4.
    Vanderhoof JA, Burkley KT, Antonson KT (1983) Potential for mucosal adaptation following massive small bowel resection in 3-week-old versus 8-week-old rats. J Pediatr Gastroenterol Nutr 2:672–676PubMedGoogle Scholar
  5. 5.
    Chiba T, Ohi R (1998 ) Do we still need to collect stool? Evaluation of visualized fatty acid absorption: experimental studies using rats. J Parenter Enteral Nutr 22:22–26Google Scholar
  6. 6.
    Molina MT, Ruiz-Cutierrez V, Vazquez CM (1990) Changes in uptake of linoleic acid and cholesterol by jejunal sacs of rats in vitro, after distal small bowel resection. Scand J Gastroenterol 25:613-621PubMedGoogle Scholar
  7. 7.
    Mok HYI, Parry PM, Dowling RH (1974) The control of bile acid pool size: effect of jejunal resection and phenobarbital on bile acid metabolism in the rats. Gut 15:247–253PubMedGoogle Scholar
  8. 8.
    Tilson MD, Boyer JL, Wright HK (1975) Jejunal absorption of bile salts after resection of the ileum. Surgery 77:231–234PubMedGoogle Scholar
  9. 9.
    Pitchumoni CS (1973) Pancreas in primary malnutrition disorders. Am J Clin Nutr 26:374–379PubMedGoogle Scholar
  10. 10.
    Weser E, Heller R, Tawil T(1977) Stimulation of mucosal growth in the rat ileum by bile and pancreatic secretion after jejunal resection. Gastroenterology 73:524–529PubMedGoogle Scholar
  11. 11.
    Menge H, Grafe M, Lorenz-Meyer H, Riecken EO (1975) The influence of food intake on the development of structural and functional adaptation following ileal resection in the rat. Gut 16:468–472.PubMedGoogle Scholar
  12. 12.
    Vanderhoof JA (1996) Short bowel syndrome. Neonatal Gastroenterol 23:377–386Google Scholar
  13. 13.
    Hart MH, Grandjean CJ, Park JHY, Erdman SH, Vanderhoof JA (1988) Essential fatty acid deficiency and postresection mucosal adaptation in the rats. Gastroenterology 94:682–687PubMedGoogle Scholar
  14. 14.
    Park JHY, Grandjean CJ, Hart MH, Vanderhoof JA (1989) Effects of dietary linoleic acid on mucosal adaptation after small bowel resection. Digestion 44:57–65PubMedGoogle Scholar
  15. 15.
    Henry RJ, Cannon DC, Winkelman JW (1974) Clinical chemistry: principles and technics, 2nd edn. Harper & Row, New York, pp 1481–1483Google Scholar
  16. 16.
    Wilson MD, Blake WL, Salati LM (1990) Potency of polyunsaturated and saturated fats as short-term inhibitors of hepatic lipogenesis in rats. J Nutr 120:544–552PubMedGoogle Scholar
  17. 17.
    Wilson HD, Schedl HP (1987) Resection of rat small intestine: calcium, phosphorus, and fat balances and 1,25-dihydroxycholecalciferol. Am J Clin Nutr 45:437–442PubMedGoogle Scholar
  18. 18.
    Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–918Google Scholar
  19. 19.
    Holman RT (1968) Essential fatty acids deficiency. Prog Chem Fats Other Lipids 9: 275-348CrossRefGoogle Scholar
  20. 20.
    Thomson AB (1986) Defined formula diets after jejunal and colonic uptake of lipids in rabbits with intact intestinal tract and following ileal resection. Res Exp Med 186:413–426Google Scholar
  21. 21.
    Booth IW, Lander AD (1998) Short bowel syndrome. Bailliere’s Clinical Gastroenterology 12:739–772Google Scholar
  22. 22.
    Wesser E (1979) Nutritional aspects of malabsorption: short gut adaptation. Am J Med 67:1014–1019PubMedGoogle Scholar
  23. 23.
    Levine GM, Deren JJ, Yezdimir E (1976) Small-bowel resection: oral intake is the stimulus for hyperplasia. Digest Dis 21:542–546Google Scholar
  24. 24.
    Ohkohchi N, Igarashi Y, Tazawa Y, Kobayashi Y, Ohi R, Kasai M (1986) Evaluation of the nutritional condition and absorptive capacity of nine infants with short bowel syndrome. J Ped Gastroenterol Nutr 5:198–206Google Scholar
  25. 25.
    Woolf GM, Miller C, Kurian R, Jeejeebhoy KN (1987) Nutritional absorption in short bowel syndrome: evaluation of fluid, calorie, and divalent cation requirements. Dig Dis Sci 32:8-15PubMedGoogle Scholar
  26. 26.
    Schwartz MK, Medwid A, Roberts KE, et al. (1955) Fat and nitrogen metabolism in patients with massive small bowel resections. Surg Forum 6:385–390Google Scholar
  27. 27.
    Singh A, Balint JA, Edmonds RH, Rodgers JB (1972) Adaptive changes of the small intestine in response to a high fat diet. Biochim Biophys Acta 260:708–715PubMedGoogle Scholar
  28. 28.
    Roshanai F, Sanders TAB (1985) Influence of different supplements of n-3 polyunsaturated fatty acids on blood and tissue lipids in rats receiving high intake of linoleic acid. Ann Nutr Metab 29:189–196PubMedGoogle Scholar
  29. 29.
    Piche LA, Mahadevappa VG (1990) Modification of rat platelet fatty acid composition by dietary lipids of animal and vegetable origin. J Nutr 120:444–449PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • I. Sukhotnik
    • 1
    • 2
  • N. Mor-Vaknin
    • 2
  • R. A. Drongowski
    • 2
  • A. G. Coran
    • 2
  • C. M. Harmon
    • 2
  1. 1.Carmel Medical CenterDept. of Surg. BHaifaIsrael
  2. 2.Section of Pediatric SurgeryC.S. Mott Children’s Hospital and University of Michigan Medical SchoolAnn ArborUSA

Personalised recommendations