Pediatric Surgery International

, Volume 20, Issue 4, pp 238–242

IL-1β induces an exaggerated pro- and anti-inflammatory response in peritoneal macrophages of children compared with adults

  • Katherine A. Barsness
  • Denis D. Bensard
  • David A. Partrick
  • Casey M. Calkins
  • Richard J. Hendrickson
  • Anirban Banerjee
  • Robert C. McIntyreJr.
Original Article

Abstract

Children have a lower incidence of acute lung injury (ALI) compared with adults. Because ALI appears to be the end result of systemic hyperinflammation, children may either have 1) an attenuated pro-inflammatory response or 2) an augmented anti-inflammatory response compared with adults. The purpose of this study was to determine the IL-1-induced pro- and anti-inflammatory response of pediatric vs. adult peritoneal macrophages (PMs). We hypothesized that pediatric PMs would have an enhanced anti-inflammatory response compared with adult PMs. Human PMs were collected during elective laparoscopic procedures, cultured, and stimulated with IL-1β. IL-6, IL-8, IL-10, and TNFα production were determined by ELISA. Statistical analyses were by ANOVA; a P <0.05 was significant. Our results showed that IL-1β induced an 11-fold increase in IL-10 production in pediatric PMs (659±103 vs. 60±25 control, P <0.05). There was no IL-10 production in IL-1β-stimulated adult PMs. IL-1β-induced TNF production was greater in children compared with adults (2152±166 vs. 592±188, P <0.05). Similarly, IL-1β-induced IL-6 production was greater in pediatric PMs compared with adults (532±3 vs. 444±52, P <0.05). There was no difference in IL-1β-induced IL-8 production in children compared with adults. The IL-10:TNFα ratio after IL-1β stimulation was 0.306±0.056 in pediatric macrophages and 0.020±0.015 in adult macrophages ( P <0.01). In conclusion, IL-1β-induced IL-6 and TNFα production were greater in pediatric than adult PMs. Furthermore, pediatric PMs had an 11-fold increase in IL-1β-induced IL-10 production, while adult PMs did not produce IL-10. Therefore, IL-1β induces both a pro- and an anti-inflammatory response in pediatric PMs, whereas adult PMs produce only pro-inflammatory cytokines in response to IL-1β. The exaggerated anti-inflammatory IL-10 response in children may be an important factor in the observed differences in ALI between children and adults.

Keywords

acute lung injury inflammatory response peritoneal macrophages IL-1β children 

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Katherine A. Barsness
    • 1
    • 2
  • Denis D. Bensard
    • 2
  • David A. Partrick
    • 2
  • Casey M. Calkins
    • 2
  • Richard J. Hendrickson
    • 2
  • Anirban Banerjee
    • 2
  • Robert C. McIntyreJr.
    • 2
  1. 1.DenverUSA
  2. 2.Division of Pediatric Surgery and GI, Tumor and Endocrine Surgery, Department of SurgeryUniversity of Colorado Health Sciences Center and The Children’s HospitalDenverUSA

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