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Child's Nervous System

, Volume 31, Issue 4, pp 581–587 | Cite as

Healing of rabbit calvarial critical-sized defects using autogenous bone grafts and fibrin glue

  • Olli-Pekka Lappalainen
  • Riikka Korpi
  • Marianne Haapea
  • Jarkko Korpi
  • Leena P. Ylikontiola
  • Soili Kallio-Pulkkinen
  • Willy S. Serlo
  • Petri Lehenkari
  • George K. Sándor
Original Paper

Abstract

Purpose

This study aimed to evaluate ossification of cranial bone defects comparing the healing of a single piece of autogenous calvarial bone representing a bone flap as in cranioplasty compared to particulated bone slurry with and without fibrin glue to represent bone collected during cranioplasty. These defect-filling materials were then compared to empty control cranial defects.

Methods

Ten White New Zealand adult male rabbits had bilateral critical-sized calvarial defects which were left either unfilled as control defects or filled with a single full-thickness piece of autogenous bone, particulated bone, or particulated bone combined with fibrin glue. The defects were left to heal for 6 weeks postoperatively before termination. CT scans of the calvarial specimens were performed. Histomorphometric assessment of hematoxylin-eosin- and Masson trichrome-stained specimens was used to analyze the proportion of new bone and fibrous tissue in the calvarial defects.

Results

There was a statistically significant difference in both bone and soft tissue present in all the autogenous bone-grafted defect sites compared to the empty negative control defects. These findings were supported by CT scan findings. While fibrin glue combined with the particulated bone seemed to delay ossification, the healing was more complete compared to empty control non-grafted defects.

Conclusions

Autogenous bone grafts in various forms such as solid bone flaps or particulated bone treated with fibrin glue were associated with bone healing which was superior to the empty control defects.

Keywords

Bone healing Cranial defect Bone graft CT 

Notes

Acknowledgments

The authors wish to express their sincere gratitude for the financial support provided to this project by the University of Oulu EVO Grant Fund and the ITI Foundation (ITI Grant Number 619-2009).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Olli-Pekka Lappalainen
    • 1
    • 2
  • Riikka Korpi
    • 3
  • Marianne Haapea
    • 3
  • Jarkko Korpi
    • 4
  • Leena P. Ylikontiola
    • 2
  • Soili Kallio-Pulkkinen
    • 3
  • Willy S. Serlo
    • 5
  • Petri Lehenkari
    • 1
  • George K. Sándor
    • 2
  1. 1.Department of Anatomy and Cell BiologyUniversity of OuluOuluFinland
  2. 2.Department of Oral and Maxillofacial SurgeryUniversity of OuluOuluFinland
  3. 3.Department of Diagnostic RadiologyOulu University HospitalOuluFinland
  4. 4.Department of OtolaryngologyOulu University HospitalOuluFinland
  5. 5.Department of Pediatric SurgeryOulu University HospitalOuluFinland

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