Child's Nervous System

, Volume 27, Issue 12, pp 2077–2085

Analysis of different medulloblastoma histotypes by two-dimensional gel and MALDI-TOF

  • Cristina Zanini
  • Giorgia Mandili
  • Daniele Bertin
  • Francesco Cerutti
  • Denisa Baci
  • Marco Leone
  • Isabella Morra
  • Luca di Montezemolo Cordero
  • Marco Forni
Original Paper
  • 194 Downloads

Abstract

Objective

The purpose of this study is to detect different protein profiles in medulloblastoma (MDB) that may be clinically relevant and to check the correspondence of histological classification of MDB with proteomic profiles.

Materials and methods

Surgical specimens, snap frozen at the time of neurosurgery, entered the proteomic study. Eight samples from patients (age range, 4 months–26 years) with different MDB histotypes (five classic, one desmoplastic/nodular, one with extensive nodularity, and one anaplastic) were analyzed by two-dimensional gel electrophoresis. One sample for each histotype was further characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis.

Results

Eighty-six unique proteins were identified and compared to histology, with the determination of proteins expressed by single histotypes and of a smaller number of proteins shared by two or three histotypes. The sharp difference of protein expression was found to be in agreement with WHO histological classification, with the identification of type-specific proteins with limited overlapping between histotypes.

Conclusion

Proteomic analysis confirmed and strengthened the difference between histotypes as biologically relevant. Cluster analysis enhanced the distance of extensive nodularity MDB from other histotypes. Possible innovative approaches to therapy may rely upon a proteomic-based classification of MDB tightly correlated to histology. The utility of snap freezing tumoral samples must be stressed and should become a mandatory task for pathologists.

Keywords

Medulloblastoma Proteomics Histology 

Supplementary material

381_2011_1515_MOESM1_ESM.doc (40 kb)
Online Resource 1Clinical data of patients (DOC 40 kb)
381_2011_1515_Fig3_ESM.jpg (153 kb)
Online Resource 2

Representative colloidal Comassie-stained 2-DE maps of MDB in four different histotypes. Proteins were separated in a pH 3-10NL strips, followed by SDS–PAGE on 10%. After mass spectrometry analysis, 86 unique proteins corresponding to 450 spots cut were identified. Identifications are reported in Tables 1 and 2. Classic MDB (a), desmoplastic/nodular MDB (b), extensive nodularity MDB (c), anaplastic MDB (d) (JPEG 153 kb)

381_2011_1515_MOESM2_ESM.tif (2.5 mb)
High resolution image file (TIFF 2532 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Cristina Zanini
    • 1
  • Giorgia Mandili
    • 2
  • Daniele Bertin
    • 3
  • Francesco Cerutti
    • 4
  • Denisa Baci
    • 1
  • Marco Leone
    • 5
  • Isabella Morra
    • 5
  • Luca di Montezemolo Cordero
    • 6
  • Marco Forni
    • 1
  1. 1.Molecular Biotechnology Centre (MBC)University of TurinTorinoItaly
  2. 2.Research Center for Experimental Medicine (CeRMS)TurinItaly
  3. 3.School of Experimental PediatricsUniversity of TurinTurinItaly
  4. 4.Department of Animal Production, Epidemiology and Ecology, MBCUniversity of Turin, ItalyTurinItaly
  5. 5.Unit of Pediatric PathologyOspedale Infantile Regina MargheritaTurinItaly
  6. 6.Unit of PediatricsUniversity of TurinTurinItaly

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