Child's Nervous System

, Volume 26, Issue 5, pp 627–636 | Cite as

Expression of neuronal antigens and related ventral and dorsal proteins in the normal spinal cord and a surgically induced open neural tube defect of the spine in chick embryos: an immunohistochemical study

  • Do-Hun Lee
  • Ji Hoon Phi
  • You-Nam Chung
  • Yun-Jin Lee
  • Seung-Ki Kim
  • Byung-Kyu Cho
  • Dong Won Kim
  • Moon-Sik Park
  • Kyu-Chang WangEmail author
Original Paper



The aims of this study were to elucidate the processes of neuronal differentiation and ventrodorsal patterning in the spinal cord of the chick embryo from embryonic day (E) 3 to E17 and to study the effect of a prenatal spinal open neural tube defect (ONTD) on these processes.


Expression patterns of neuronal antigens (neuronal nuclear antigen, neurofilament-associated protein (NAP), and synaptophysin) and related ventral markers [sonic hedgehog, paired box gene (PAX)6, and islet-1], and dorsal markers (bone morphogenetic protein, Notch homolog 1, and PAX7) were investigated in the normal spinal cord and in a surgically induced spinal ONTD in chick embryos. Four normal and ONTD chick embryos were used for each antigen group.

Result & Conclusion

There were no differences in the expression of neuronal and ventrodorsal markers between the control and ONTD groups. NAP and synaptophysin were useful for identifying dorsal structures in the distorted anatomy of the ONTD chicks.


Spinal cord Chick embryo Open neural tube defect Neuron Ventral and dorsal markers 



This study was supported by a Seoul National University Hospital Research Grant (no. 04-2002-008-0).


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Do-Hun Lee
    • 1
  • Ji Hoon Phi
    • 1
  • You-Nam Chung
    • 1
  • Yun-Jin Lee
    • 1
  • Seung-Ki Kim
    • 1
  • Byung-Kyu Cho
    • 1
  • Dong Won Kim
    • 2
  • Moon-Sik Park
    • 2
  • Kyu-Chang Wang
    • 1
    • 3
    Email author
  1. 1.Division of Pediatric NeurosurgerySeoul National University Children’s HospitalSeoulSouth Korea
  2. 2.Department of NeurosurgeryKeimyung University School of MedicineDaeguSouth Korea
  3. 3.Department of NeurosurgerySeoul National University College of MedicineSeoulSouth Korea

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