Upregulation of mir-221 and mir-222 in atypical teratoid/rhabdoid tumors: potential therapeutic targets
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The aim of this study is to search for new therapeutic targets for atypical teratoid–rhabdoid tumors (ATRT).
To achieve this, we compared the expression of 365 microRNAs among ATRT, medulloblastomas, and normal brain.
MiR-221 and miR-222 were within the top differentially expressed microRNAs. The deregulated expression of miR221/222 was demonstrated to inhibit the expression of the tumor suppressor and inhibitor of cell cycle p27Kip1. Here, we demonstrated the negative regulation of p27Kip1 by miR-221/222 in ATRT using microarray, real-time reverse transcriptase polymerase chain reaction, and immunohistochemistry.
As anti-miR therapy was recently proposed as an alternative treatment for cancer, these findings suggest that anti-miR-221/222 therapy might have therapeutic potential in ATRT.
KeywordsAtypical teratoid/rhabdoid tumors miR221 miR222 p27Kip1
This project was supported by the Dr. Ralph and Marian C. Falk Medical Research Trust, the Rally Foundation for Childhood Cancer Research, and the Maeve McNicholas Memorial Foundation.
This study has been approved by the Children’s Memorial Hospital Institutional Review Board (IRB#2009-13778) and had therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki. All persons gave their informed consent prior to their inclusion in the study.
Conflict of interest
The authors declare that they have no conflict of interest.
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