Child's Nervous System

, Volume 25, Issue 1, pp 13–20

Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis

  • Jian-Jun Zhao
  • Juhua Yang
  • Jianhong Lin
  • Nan Yao
  • Yihua Zhu
  • Jianlong Zheng
  • Jianhua Xu
  • Jin Q. Cheng
  • Jian-Yin Lin
  • Xu Ma
Original Paper

DOI: 10.1007/s00381-008-0701-x

Cite this article as:
Zhao, JJ., Yang, J., Lin, J. et al. Childs Nerv Syst (2009) 25: 13. doi:10.1007/s00381-008-0701-x

Abstract

Introduction

MicroRNAs (miRNAs) are small (∽22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.

Methods

This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.

Results

A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513–1, hsa-miR-513–2, hsa-miR-518c*, hsa-miR-129–1, hsa-miR-129–2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.

Conclusion

These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.

Keywords

MicroRNAs Retinoblastoma Microarray In situ hybridization Locked nucleic acid 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jian-Jun Zhao
    • 1
    • 2
    • 6
  • Juhua Yang
    • 2
    • 3
  • Jianhong Lin
    • 1
    • 6
  • Nan Yao
    • 1
    • 2
  • Yihua Zhu
    • 4
  • Jianlong Zheng
    • 4
  • Jianhua Xu
    • 3
  • Jin Q. Cheng
    • 6
  • Jian-Yin Lin
    • 5
  • Xu Ma
    • 1
    • 2
  1. 1.Peking Union Medical CollegeTsinghua UniversityBeijingChina
  2. 2.Department of Genetics, National Research Institute for Family Planning, Peking Union Medical CollegeTsinghua UniversityBeijingChina
  3. 3.Biomedical Engineering CenterFujian Medical UniversityFuzhouChina
  4. 4.Department of OphthalmologyThe First Affiliated Hospital of Fujian Medical UniversityFuzhouChina
  5. 5.Key Laboratory of Infection and Oncology, Molecular Medicine Research Center of Fujian Medical UniversityFujian Province UniversityFuzhouChina
  6. 6.H. Lee Moffitt Cancer Center and Research InstituteUniversity of South FloridaTampaUSA

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