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Child's Nervous System

, Volume 24, Issue 9, pp 987–994 | Cite as

Caffeic acid phenethyl ester preferentially enhanced radiosensitizing and increased oxidative stress in medulloblastoma cell line

  • Yi-Yen Lee
  • Chung-Lan Kao
  • Ping-Hsing Tsai
  • Tung-Hu Tsai
  • Shih-Hwa Chiou
  • Wei-Fong Wu
  • Hung-Hai Ku
  • Tai-Tong Wong
Original Paper

Abstract

Objectives

Caffeic acid phenethyl ester (CAPE), an active component of propolis, was recently reported to have radiosensitizing effects on medulloblastoma (MB) cells. However, the mechanisms of radiosensitivity involved in medulloblastoma cells are still unclear. The specific aim of this study was to investigate the role of CAPE-induced oxidative stress to influence of radiosensitivity and anti-proliferative effects in medulloblastoma cells.

Materials and methods

Medulloblastoma (Daoy) cells were treated with CAPE in different concentrations and assessed for cell viability. The following were also evaluated: migratory ability, reduced glutathione (GSH) level, reactive oxygen species (ROS) level, nuclear factor-kappaB (NF-κB) activity, and apoptosis in CAPE alone, radiation alone, or radiation combined with CAPE in Daoy cells.

Results

The results indicated that CAPE inhibited the growth of Daoy cells. CAPE treatment in Daoy cells could effectively decrease glutathione reductase and significantly increase glutathione peroxidase. Radiation-activated NF-κB was reversed by CAPE pretreatment. Finally, the result of terminal deoxynucleotidyl transferase-mediated dUTP–biotin nick end labeling assay showed that CAPE treatment can enhance radiation-induced apoptosis in Daoy cells.

Conclusions

Our study demonstrated the anti-proliferative and radiosensitizing effects of CAPE on MB cells, which may be achievable through depleting GSH, increased ROS activity, and inhibiting NF-κB activity.

Keywords

Caffeic acid phenethyl ester (CAPE) Medulloblastoma Radiosensitivity Nuclear factor-kappaB (NF-κB) Glutathione (GSH) Reactive oxygen species (ROS) 

Notes

Acknowledgment

This study was supported by research grants from the National Science Council (NSC-96-3111-B-075-001, 96-2628-B-010-006-MY3), Taipei Veterans General Hospital (V97E1-008, V97F-001), Yen-Tjing-Ling Medical Foundation, Taipei City Hospital (96001-62-014, 96001-62-018, 96002-62-092), and National Yang-Ming University (Ministry of Education, Aim for the Top University Plan), Taiwan.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Yi-Yen Lee
    • 1
    • 7
  • Chung-Lan Kao
    • 1
    • 4
  • Ping-Hsing Tsai
    • 2
    • 5
  • Tung-Hu Tsai
    • 3
    • 8
  • Shih-Hwa Chiou
    • 1
    • 5
  • Wei-Fong Wu
    • 7
  • Hung-Hai Ku
    • 2
  • Tai-Tong Wong
    • 6
  1. 1.Institute of Clinical MedicineNational Yang-Ming UniversityTaipeiTaiwan
  2. 2.Institute of Anatomy and Cell BiologyNational Yang-Ming UniversityTaipeiTaiwan
  3. 3.Institute of Traditional MedicineNational Yang-Ming UniversityTaipeiTaiwan
  4. 4.Department of Physical Medicine and Rehabilitation, The Neurological InstituteTaipei Veterans General HospitalTaipeiTaiwan
  5. 5.Department of Education and Research, The Neurological InstituteTaipei Veterans General HospitalTaipeiTaiwan
  6. 6.Division of Pediatric Neurosurgery, The Neurological InstituteTaipei Veterans General HospitalTaipeiTaiwan
  7. 7.Department of PediatricTaipei City Hospital, Renai BranchTaipeiTaiwan
  8. 8.Department of Education and ResearchTaipei City Hospital, Renai BranchTaipeiTaiwan

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