Linc00299/miR-490-3p/AURKA axis regulates cell growth and migration in atherosclerosis
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Long non-coding RNA (lncRNA) plays a crucial role in regulating various cellular processes in atherosclerosis. The present study identified the regulation of Linc00299, via miR-490-3p targeting Aurora kinase A (AURKA), on migration and proliferation of endothelial cells and vascular smooth muscle cells (VSMCs) during atherosclerosis. The expression of RNAs was assessed by real-time PCR. The proliferation, apoptosis and migration were detected using MTT assay, Annexin V/PI staining and Transwell system, respectively. Bindings of Linc00299/miR-490-3p and subsequent miR-490-3p/AURKA were verified by luciferase and biotin pull-down assays. The protein expression of AURKA was detected by Western blotting. Expressions of Linc00299 and miR-490-3p were upregulated and downregulated in atherosclerosis patients, respectively. Both Linc00299 knockdown and miR-490-3p overexpression suppressed cell proliferation, increased apoptosis and inhibited migration of VSMCs and HUVECs. Linc00299 directly bound to miR-490-3p which targeted AURKA. The regulation of Linc00299 on expression of AURKA and proliferation and migration of VSMCs were dependent on miR-490-3p. Atherosclerosis-increased Linc00299 acts as a sponge of miR-490-3p to upregulate AURKA, and as a result increases proliferation and migration in VSMCs and HUVECs. Our study reveals an important effect of Linc00299/miR-490-3p/AURKA axis on regulating cell proliferation and migration in atherosclerosis.
KeywordsAtherosclerosis Linc00299 miR-490-3p AURKA Vascular smooth muscle cells
This study was supported by the Health Planning Commission of Hebei Provincial Medical Research (NO 20180724), Baoding Science and Technology Project (NO 17ZF131) and Health Planning Commission and Finance Department of Hebei Provincial: Geriatric Disease Prevention and Control Project (NO 2017068879-204).
Compliance with ethical standards
Conflicts of interest
All authors disclose that they do not have any financial and personal relationships with other people or organizations that could inappropriately influence (bias) their work. Potential conflicts of interest include employment, consultancies, stock ownership, honoraria, paid expert testimony, patent applications/registrations and grants or other funding. All the authors state that they have no conflicts of interest.
Human and animal rights
The study was approved by the Ethics Committee of Affiliated Hospital of Hebei University.
Written informed consent had been signed by all patients.
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