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Heart and Vessels

, Volume 34, Issue 3, pp 538–544 | Cite as

Accumulation of gold nano-rods in the failing heart of transgenic mice with the cardiac-specific expression of TNF-α

  • Yoshihiro HiguchiEmail author
  • Takuro Niidome
  • Yuji Miyamoto
  • Yoshihiro Komohara
  • Tomotake Tokunou
  • Toru Kubota
  • Takahiko Horiuchi
Original Article
  • 140 Downloads

Abstract

Gold nano-rods, rod-shaped gold nanoparticles, act as contrast agents for in vivo bioimaging, drug delivery vehicles and thermal converters for photothermal therapy. Pro-inflammatory cytokines play critical roles in the development of heart failure. We examined the delivery of GNRs into the failing heart of a transgenic (TG) mouse model of inflammatory cardiomyopathy with the cardiac-specific overexpression of TNF-α. We modified GNRs with polyethylene glycol (PEG) to avoid cytotoxicity and reduce the rapid clearance of nanoparticles from blood. PEG-modified GNRs (4.5 mM as gold atoms, 200 μL) were administered intravenously to TG (n = 7) and wild-type (WT) mice (n = 5). These were killed 24 h later, and the heart, lung, liver, kidney and spleen were excised. A quantitative analysis of gold was performed using inductively coupled plasma mass or optical emission spectrometry. The amount of gold (ng) in the TG heart (3.24 ± 1.56 ng/mg heart weight) was significantly greater than that in the WT heart (1.01 ± 0.19; p < 0.05). No significant differences were observed among the other organs of TG and WT mice. The amount of gold in the TG heart was significantly and positively correlated with the ratio of the ventricular weight to body weight, which is known to be an index of ventricular hypertrophy. In conclusion, PEG-modified GNRs accumulated in the inflammatory TG heart in proportion with the severity of ventricular hypertrophy.

Keywords

Gold nano-rod Heart failure Inflammation TNF-α 

Notes

Acknowledgements

The authors thank K. Tsuchida for her expert technical assistance.

Compliance with ethical standards

Ethical approval

All procedures performed in this study involving animals were in accordance with the ethical standards of our institution.

Conflict of interest

The authors declare no conflicts of interest in association with the present study.

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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Yoshihiro Higuchi
    • 1
    Email author
  • Takuro Niidome
    • 2
  • Yuji Miyamoto
    • 2
  • Yoshihiro Komohara
    • 3
  • Tomotake Tokunou
    • 1
  • Toru Kubota
    • 4
  • Takahiko Horiuchi
    • 1
  1. 1.Department of Internal MedicineKyushu University Beppu HospitalBeppuJapan
  2. 2.Department of Applied Chemistry and Biochemistry, Faculty of Advanced Science and TechnologyKumamoto UniversityKumamotoJapan
  3. 3.Department of Cell Pathology, Graduate School of Medical SciencesKumamoto UniversityKumamotoJapan
  4. 4.Department of CardiologySaiseikai Fukuoka General HospitalFukuokaJapan

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