Heart and Vessels

, Volume 32, Issue 12, pp 1536–1545 | Cite as

Tumor necrosis factor alpha-stimulated gene-6 (TSG-6) inhibits the inflammatory response by inhibiting the activation of P38 and JNK signaling pathway and decreases the restenosis of vein grafts in rats

  • Chengxin Zhang
  • Biao Zhang
  • Huiping Wang
  • Qianshan Tao
  • Shenglin GeEmail author
  • Zhimin ZhaiEmail author
Original Article


This study aims to explore the effects of tumor necrosis factor alpha-stimulated gene-6 (TSG-6) on vascular inflammatory response and vascular injury in grafted vein wall of rats and its possible mechanism. Vascular grafting model was established by modified cuff. The effect of TSG-6 on the inflammatory response and vascular injury of vein graft was investigated. The activation of mast cells and macrophages after LPS stimulation was observed by lentivirus-mediated upregulation or downregulation of TSG-6 expression. The results showed that rhTSG-6 treatment could significantly inhibit the proliferation of venous bridge, decrease macrophage infiltration and smooth muscle cell proliferation. The expression levels of TNF-α and IL-1 in treated group were significantly lower than that of untreated group (P < 0.05), while the expression of IL-10 in treated group were significantly higher than that of untreated group (P < 0.05). The expression levels of P38, p-P38, JNK and p-JNK in venous bridge of rats were significantly lower than those of untreated rats (P < 0.05), while there was no significant difference in the expression level of ERK and p-ERK (P > 0.05). TSG-6 could inhibit the proliferation of mast cells and macrophages and the release of inflammatory cytokines by down regulating the expression levels of P38, p-P38, JNK and p-JNK. TSG-6 can inhibit the inflammatory response of transplanted vein grafts in rats and reduce vascular injury by downregulation of P38 and JNK signaling pathway.


Tumor necrosis factor alpha-stimulated gene-6 (TSG-6) Grafted vein Venous bridge Proinflammatory response 


Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interests.


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Copyright information

© Springer Japan KK 2017

Authors and Affiliations

  1. 1.Cardiac Surgery DepartmentThe 1st Affiliated Hospital of Anhui Medical UniversityHefeiChina
  2. 2.Hematology DepartmentThe 2nd Affiliated Hospital of Anhui Medical UniversityHefeiChina

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