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Heart and Vessels

, Volume 29, Issue 5, pp 718–722 | Cite as

Reduction of inorganic phosphate-induced human smooth muscle cells calcification by inhibition of protein kinase A and p38 mitogen-activated protein kinase

  • Jeong-Hun KangEmail author
  • Riki Toita
  • Daisuke Asai
  • Tetsuji Yamaoka
  • Masaharu Murata
Short Communication

Abstract

High levels of serum phosphate are associated with calcification of human smooth muscle cells (HSMCs). We investigated whether inhibition of protein kinase A (PKA) and mitogen-activated protein kinase (MAPK) signals [p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK)] can reduce inorganic phosphate (Pi)-induced HSMC calcification. Inhibition of PKA or p38 MAPK by inhibitors or small interfering RNAs (siRNAs) reduced Ca levels and alkaline phosphatase activities in HSMCs treated with high Pi, but inhibition of ERK1/2 and JNK showed no significant changes. Moreover, there were no significant changes in cell viability on adding siRNAs and three inhibitors (PKA, p38, and MEK1/2), but JNK inhibitor slightly reduced cell viability. These results show that PKA and p38 MAPK are involved in the Pi-induced calcification of HSMCs, and may be good targets for reducing vascular calcification.

Keywords

Smooth muscle cell Calcification Protein kinase A Mitogen-activated protein kinase Alkaline phosphatase 

Notes

Acknowledgments

This work was financially supported by a grant-in-aid for Scientific Research (B) (KAKENHI Grant Number 23310085) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.

Supplementary material

380_2013_427_MOESM1_ESM.pdf (214 kb)
Supplementary material 1 (PDF 213 kb)

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Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Jeong-Hun Kang
    • 1
    Email author
  • Riki Toita
    • 2
  • Daisuke Asai
    • 3
  • Tetsuji Yamaoka
    • 1
  • Masaharu Murata
    • 4
  1. 1.Department of Biomedical EngineeringNational Cerebral and Cardiovascular Center Research InstituteSuitaJapan
  2. 2.Department of Biomaterials, Faculty of Dental ScienceKyushu UniversityFukuokaJapan
  3. 3.Department of MicrobiologySt. Marianna University School of MedicineKawasakiJapan
  4. 4.Department of Advanced Medical Initiatives, Faculty of Medical SciencesKyushu UniversityFukuokaJapan

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