Biology and Fertility of Soils

, Volume 33, Issue 2, pp 152–156 | Cite as

Isolation and identification of the most efficient plant growth-promoting bacteria associated with canola (Brassica napus)

  • H. Bertrand
  • R. Nalin
  • R. Bally
  • J.-C. Cleyet-Marel
ORIGINAL PAPER

Abstract

 Thirteen of the most abundant Gram-negative bacteria which are able to grow in N-depleted culture conditions were isolated from the rhizoplane and endorhizosphere of canola (Brassica napus) and identified by 16S rDNA sequence analysis. Eight of these bacteria induced a significant increase in root dry weight ranging from 11 to 52%. Phylogenetic positioning based on 16S rDNA sequences indicated that at least four genera are represented, Pseudomonas, Variovorax, Agrobacterium and Phyllobacterium. The most important direct plant growth-promoting-rhizobacteria effect was found with both isolates belonging to the Phyllobacterium.

Keywords Direct plant growth-promoting rhizobacteria effect Rhizobacteria Canola Phyllobacterium 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • H. Bertrand
    • 2
  • R. Nalin
    • 1
  • R. Bally
    • 1
  • J.-C. Cleyet-Marel
    • 2
  1. 1.L.E.M.S. UMR-CNRS 5557, Laboratoire d'Ecologie Microbienne du Sol, Bat. 741, 4eme et., Université Claude Bernard Lyon I, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France e-mail: nalin@biomserv.univ-lyon1.fr Tel.: +33-4-72431110 Fax: +33-4-72431223FR
  2. 2.L.S.T.M. Laboratoire des Symbioses Tropicales et Méditérannéennes, INRA/IRD/CIRAD/AGRO-M TA10/J Campus International de Baillarguet 34398 Montpellier cedex 5, FranceFR

Personalised recommendations