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Bacterial communities involved directly or indirectly in the anaerobic degradation of cellulose

  • Yuanyuan Bao
  • Jan Dolfing
  • Baozhan Wang
  • Ruirui Chen
  • Miansong Huang
  • Zhongpei Li
  • Xiangui Lin
  • Youzhi FengEmail author
Original Paper
  • 133 Downloads

Abstract

To determine bacterial communities involved, directly or indirectly, in the anaerobic degradation of cellulose, we conducted a microcosm experiment with soil treated with 13C-cellulose, 12C-cellulose, or without cellulose with analyses of DNA-based stable isotope probing (DNA-SIP), real-time quantitative PCR, and high-throughput sequencing. Firmicutes, Actinobacteria, Verrucomicrobia, and Fibrobacteres were the dominant bacterial phyla-degrading cellulose. Generally, bacteria possessing gene-encoding enzymes involved in the degradation of cellulose and hemicellulose were stimulated. Phylotypes affiliated to Geobacter were also stimulated by cellulose, probably due to their role in electron transfer. Nitrogen-fixing bacteria were also detected, probably due to the decreased N availability during cellulose degradation. High-throughput sequencing showed the presence of bacteria not incorporating 13C and probably involved in the priming effect caused by the addition of cellulose to soil. Collectively, our findings revealed that a more diverse microbial community than expected directly and indirectly participated in anaerobic cellulose degradation.

Keywords

Cellulose degradation Paddy soil Cellulolytic bacteria Syntrophic microorganisms Nitrogen-fixing bacteria Priming effect 

Notes

Acknowledgements

The authors thank Ms. Yushan Zhan for her assistance of material preparation before the experiment. Authors also thank Editor-in-Chief Prof. Paolo Nannipieri and three anonymous reviewers for their constructive comments and suggestions, which greatly improved the manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (Project Nos. 41430859, 41771294, 41671267, and 41471208), the CAS Strategic Priority Research Program Grant (Project No. XDB15020103), National Key R&D Program (2016YFD0200306), Research Program for Key Technologies of Sponge City Construction and Management in Guyuan City (Grant No. SCHM-2018), and Knowledge Innovation Program of Chinese Academy of Sciences (Grant No. ISSASIP1639).

Supplementary material

374_2019_1342_MOESM1_ESM.docx (200 kb)
ESM 1 (DOCX 199 kb)
374_2019_1342_MOESM2_ESM.xlsx (12 kb)
Supplementary Table S2 (XLSX 11 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yuanyuan Bao
    • 1
    • 2
  • Jan Dolfing
    • 3
  • Baozhan Wang
    • 1
  • Ruirui Chen
    • 1
  • Miansong Huang
    • 4
  • Zhongpei Li
    • 1
  • Xiangui Lin
    • 1
  • Youzhi Feng
    • 1
    Email author
  1. 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of SciencesNanjingPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.School of Engineering, Newcastle UniversityNewcastle-upon-TyneUK
  4. 4.Beijing Capital Co., LTDBeijingPeople’s Republic of China

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