Effects of O3 stress on physico-chemical and biochemical properties and composition of main microbial groups of a soil cropped to soybean
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Tropospheric O3 (ozone) stress can negatively affect forest productivity and crop yields. Yet, relatively little attention has been paid to the effects of O3 stress on belowground system. Here, a pot experiment was conducted in open top chambers to monitor the response of physico-chemical properties, main microbial groups, and potential enzyme activities of a soil cropped to soybean (Glycine max; a highly sensitive species to O3) and exposed to background O3 concentration (45 ± 5 ppb, control) and O3 stress (80 ± 10 ppb, O3+ and 110 ± 10 ppb, O3++) with sampling at branching, flowering, and podding stages. The growth of soybean was significantly inhibited by O3 stress, which showed significant effects on soil microbial biomass C and pH during the whole growth of soybean at the highest concentration. The O3++ stress significantly decreased soil pH at flowering stage, and increased soil pH at podding stage; the O3+ stress and growth stage × O3+ stress showed significant influences on the potential activities of acid phosphomonoesterase, invertase, and amylase. The O3 stress significantly reduced the abundances of total PLFAs (phospholipid fatty acid), bacterial PLFAs, and AMF (arbuscular mycorrhizal) PLFAs at branching and podding stages. Our results suggest that the main soil microbial groups might be indirectly affected by the O3 stress through the alteration of soil physico-chemical properties with changes in the potential enzyme activities of soil.
KeywordsSoybean root Growth stage Phospholipid fatty acid (PLFA) Potential enzyme activity Pot experiment Open top chamber (OTC)
This work was supported by the National Natural Science Foundation of China (30970448; 31570404) and China Postdoctoral Science Foundation (2016M601342). We thank editor, three anonymous referees, Gui-Gang Lin PhD and De-Hui Zeng professor for their valuable comments and suggestions that greatly improved the manuscript.
- Carter MR, Gregorich EG (2007) Soil sampling and methods of analysis, 2nd ed. CRC Press, Taylor & Francis Group, Boca RatonGoogle Scholar
- Cole MA (1977) Lead inhibition of enzyme synthesis in soil. Appl Environ Microbiol 3:262–268Google Scholar
- Lepš J, Šmilauer P (2003) Multivariate analysis of ecological data using CANOCO. Cambridge University PressGoogle Scholar
- Meehl GA, Stocker TF, Collins WD, Friedlingstein P, Gaye AT, Gregory JM, Kitoh A, Knutti R, Murphy JM, Noda A, Raper SCB, Watterson IG, Weaver AJ, Zhao ZC (2007) Climate change 2007: the physical science basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USAGoogle Scholar
- Nelson DW, Sommers LE (1982) Total carbon, organic carbon, and organic matter. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis. American Society of Agronomy and Soil Science Society of American, MadisonGoogle Scholar
- Pritsch K, Ernst D, Fleischmann F, Gayler S, Grams TEE, Göttlein A, Heller W, Koch N, Lang H, Matyssek R, Munch JC, Olbrich M, Scherb H, Stich S, Winkler JB, Schloter M (2008) Plant and soil system responses to ozone after 3 years in a lysimeter study with juvenile beech (Fagus sylvatica, L.). Water Air Soil Pollut 8:139–154CrossRefGoogle Scholar
- Tabatabai MA (1982) Soil enzymes in methods of soil analysis. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis. American Society of Agronomy and Soil Science of America, Madison, pp 903–947Google Scholar