Advertisement

Biology and Fertility of Soils

, Volume 54, Issue 6, pp 783–789 | Cite as

Phosphorus addition enhances gross microbial N cycling in phosphorus-poor soils: a 15N study from two long-term fertilization experiments

  • Yi Cheng
  • Jing Wang
  • Nan Sun
  • Minggang Xu
  • Jinbo Zhang
  • Zucong Cai
  • Shenqiang Wang
Short Communication

Abstract

The tight coupling between nitrogen (N) and phosphorus (P) suggests that P availability may affect soil microbial N dynamics in terrestrial ecosystems. However, how P addition affects the internal N transformations in P-deficient agricultural soil remains poorly understood. We hypothesized that an increase in gross microbial N rates in P-deficient soil should occur after long-term P inputs in agricultural soils. We thus conducted a 15N pool dilution experiment to quantify the gross microbial N transformation rates after long-term mineral fertilizer applications in an upland fluvo-aquic soil (from Fengqiu with pH 8.55) and upland red soil (from Qiyang with pH 5.49) in China. We found that P addition significantly enhanced the gross N mineralization and immobilization rates when N and K were also applied, probably due to the increased soil total C and N concentrations at both soils. Also, gross nitrification rate was stimulated by P addition, perhaps because of enhanced gross N mineralization rates and associated NH4+ substrate availability. Our results showed that long-term P addition may stimulate soil gross N dynamics and hence increase overall N availability for crops in P-deficient agricultural soils.

Keywords

Phosphorus deficiency P availability N dynamics Gross N mineralization 15N recovery 

Notes

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (grant numbers 41671231, 41571294), the High-Level Talent Start-Up Research Project of Nanjing Forestry University (grant numbers GXL2018012), and the National Key Research and Development Program of China (grant numbers 2017YFD0200103, 2017YFD0800106).

Supplementary material

374_2018_1294_MOESM1_ESM.docx (115 kb)
ESM 1 (DOCX 115 kb)

References

  1. Bai ZH, Li HG, Yang XY, Zhou BK, Shi XJ, Wang BR, Li DC, Shen JB, Chen Q, Qin W (2013) The critical soil P levels for crop yield: soil fertility and environmental safety in different soil types. Plant Soil 372:27–37CrossRefGoogle Scholar
  2. Bauhus J, Khanna PK (1994) Carbon and nitrogen turnover in two acid forest soils of Southeast Australia as affected by phosphorus addition and drying and wetting cycles. Biol Fertil Soils 17:212–218CrossRefGoogle Scholar
  3. Booth MS, Stark JM, Rastetter E (2005) Controls on nitrogen cycling in terrestrial ecosystems: a synthetic analysis of literature data. Ecol Monogr 75:139–157CrossRefGoogle Scholar
  4. Bremner JM (1996) Nitrogen-total. In: Sparks DL (ed) Methods of soil analysis. Part 3. Chemical methods. Soil Science Society of America, Madison, pp 1085–1121Google Scholar
  5. Cai ZJ, Wang BR, Xu MG, Zhang HM, He XH, Zhang L, Gao SD (2015) Intensified soil acidification from chemical N fertilization and prevention by manure in an 18-year field experiment in the red soil of southern China. J Soils Sediments 15:260–270CrossRefGoogle Scholar
  6. Chen Y, Sun TT, Qian HY, Fan JB, He YQ, Sun B (2016) Nitrogen mineralization as a result of phosphorus supplementation in long-term phosphate deficient soil. Appl Soil Ecol 106:24–32CrossRefGoogle Scholar
  7. Chen H, Zhang W, Gurmesa GA, Zhu X, Li D, Mo J (2017) Phosphorus addition affects soil nitrogen dynamics in a nitrogen-saturated and two nitrogen-limited forests. Eur J Soil Biol 68:472–479Google Scholar
  8. Cheng Y, Wang J, Zhang JB, Müller C, Wang SQ (2015) Mechanistic insights into the effects of N fertilizer application on soil N2O emission pathways in acidic soil of a tea plantations. Plant Soil 389:45–57CrossRefGoogle Scholar
  9. Cui S, Shi Y, Groffman PM, Schlesinger WH, Zhu YG (2013) Centennial-scale analysis of the creation and fate of reactive nitrogen in China (1910–2010). Proc Natl Acad Sci U S A 110:2052–2057CrossRefPubMedPubMedCentralGoogle Scholar
  10. de Groot C, Marcelis LM, van den Boogaard R, Kaiser W, Lambers H (2003) Interaction of nitrogen and phosphorus nutrition in determining growth. Plant Soil 248:257–268CrossRefGoogle Scholar
  11. Erguder TH, Boon N, Wittebolle L, Marzorati M, Verstraete W (2009) Environmental factors shaping the ecological niches of ammonia oxidizing archaea. FEMS Microbiol Rev 33:855–869CrossRefPubMedGoogle Scholar
  12. Guo JH, Liu XJ, Zhang Y, Shen JL, Han WX, Zhang WF, Christie P, Goulding KWT, Vitousek PM, Zhang FS (2010) Significant acidification in major Chinese croplands. Science 327:1008–1010CrossRefPubMedGoogle Scholar
  13. He M, Dijkstra FA (2015) Phosphorus addition enhances loss of nitrogen in a phosphorus-poor soil. Soil Biol Biochem 82:99–106CrossRefGoogle Scholar
  14. He D, Xiang XJ, He JS, Wang C, Cao GM, Adams J, Chu HY (2016) Composition of the soil fungal community is more sensitive to phosphorus than nitrogen addition in the alpine meadow on the Qinghai–Tibetan plateau. Biol Fertil Soils 52:1059–1072CrossRefGoogle Scholar
  15. Hu J, Lin X, Wang J, Chu H, Yin R, Zhang J (2009) Population size and specific potential of P-mineralizing and -solubilizing bacteria under long-term P-deficiency fertilization in a sandy loam soil. Pedobiologia 53:49–58CrossRefGoogle Scholar
  16. Jing ZW, Chen RR, Wei SP, Feng YZ, Zhang JB, Lin XG (2017) Response and feedback of C mineralization to P availability driven by soil microorganisms. Soil Biol Biochem 105:111–120CrossRefGoogle Scholar
  17. Ju XT, Xing GX, Chen XP, Zhang SL, Zhang LJ, Liu XJ, Cui ZL, Yin B, Christie P, Zhu ZL, Zhang FS (2009) Reducing environmental risk by improving N management in intensive Chinese agricultural systems. Proc Natl Acad Sci U S A 106:3041–3046CrossRefPubMedPubMedCentralGoogle Scholar
  18. Kirkham D, Bartholomew WV (1954) Equations for following nutrient transformations in soil utilizing tracer data. Soil Sci Soc Am J 18:33–34CrossRefGoogle Scholar
  19. Landi L, Badalucco L, Pomaré F, Nannipieri P (1993) Effectiveness of antibiotics to distinguish the contributions of fungi and bacteria net nitrogen mineralisation, nitrification and respiration. Soil Biol Biochem 25:1771–1778CrossRefGoogle Scholar
  20. Lang M, Li P, Han XZ, Qiao YF, Miao SJ (2016) Gross nitrogen transformations in black soil under different land uses and management systems. Biol Fertil Soils 52:233–241CrossRefGoogle Scholar
  21. Li J, Li Z, Wang F, Zou B, Chen Y, Zhao J, Mo Q, Li Y, Li X, Xia H (2015) Effects of nitrogen and phosphorus addition on soil microbial community in a secondary tropical forest of China. Biol Fertil Soils 51:207–215CrossRefGoogle Scholar
  22. Liu L, Gundersen P, Zhang T, Mo J (2012) Effects of phosphorus addition on soil microbial biomass and community composition in three forest types in tropical China. Soil Biol Biochem 44:31–38CrossRefGoogle Scholar
  23. Liu L, Zhang T, Gilliam FS, Gundersen P, Zhang W, Chen H, Mo J (2013) Interactive effects of nitrogen and phosphorus on soil microbial communities in a tropical forest. PLoS One 8:e61188CrossRefPubMedPubMedCentralGoogle Scholar
  24. Mehnaz KR, Dijkstra FA (2016) Denitrification and associated N2O emissions are limited by phosphorus availability in a grassland soil. Geoderma 284:34–41CrossRefGoogle Scholar
  25. Mehnaz KR, Keitel C, Dijkstra FA (2018) Effects of carbon and phosphorus addition on microbial respiration, N2O emission, and gross nitrogen mineralization in a phosphorus-limited grassland soil. Biol Fertil Soils 54:481–493.  https://doi.org/10.1007/s00374-018-1274-9 CrossRefGoogle Scholar
  26. Mori T, Yokoyama D, Kitayama K (2016) Contrasting effects of exogenous phosphorus application on N2O emissions from two tropical forest soils with contrasting phosphorus availability. SpringerPlus 5:1237CrossRefPubMedPubMedCentralGoogle Scholar
  27. Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27:31–36CrossRefGoogle Scholar
  28. Murphy DV, Recous S, Stockdale EA, Fillery IRP, Jensen LS, Hatch DJ, Goulding KWT (2003) Gross nitrogen fluxes in soil: theory, measurement and application of 15N pool dilution techniques. Adv Agron 79:69–118CrossRefGoogle Scholar
  29. Neumann G, Römheld V (2002) Root-induced changes in the availability of nutrients in the rhizosphere. In: Waisel Y, Eshel A, Kafkafi U (eds) Plant roots, the hidden half, 3rd edn. CRC Press, New York, pp 617–649CrossRefGoogle Scholar
  30. Shi Y, Lalande R, Ziadi N, Sheng M, Hu Z (2012) An assessment of the soil microbial status after 17 years of tillage and mineral P fertilization management. Appl Soil Ecol 62:14–23CrossRefGoogle Scholar
  31. Vervaet H, Boeckx P, Boko AMC, Van Cleemput O, Hofman G (2004) The role of gross and net N transformation processes and NH4 + and NO3 immobilization in controlling the mineral N pool of a temperate mixed deciduous forest soil. Plant Soil 264:349–357CrossRefGoogle Scholar
  32. Wei XM, Hu YJ, Peng PQ, Zhu ZK, Atere CT, O’Donnell AG, Wu JS, Ge TD (2017) Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil. Biol Fertil Soils 53:767–776CrossRefGoogle Scholar
  33. Zhang F, Shen J, Zhang J, Zuo Y, Li L, Chen X (2010) Rhizosphere processes and management for improving nutrient use efficiency and crop productivity: implications for China. Adv Agron 107:1–32CrossRefGoogle Scholar
  34. Zhang JB, Zhu TB, Cai ZC, Qin SW, Müller C (2012) Effects of long-term repeated mineral and organic fertilizer applications on soil nitrogen transformations. Eur J Soil Biol 63:75–85Google Scholar
  35. Zhou Z, Shi X, Zheng Y, Qin Z, Xie D, Li Z, Guo T (2014) Abundance and community structure of ammonia-oxidizing bacteria and archaea in purple soil under long-term fertilization. Eur J Soil Biol 60:24–33CrossRefGoogle Scholar
  36. Zhou ZH, Wang CK, Jing Y (2017) Stoichiometric responses of soil microflora to nutrient additions for two temperate forest soils. Biol Fertil Soils 53:397–406CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yi Cheng
    • 1
  • Jing Wang
    • 2
  • Nan Sun
    • 3
  • Minggang Xu
    • 3
  • Jinbo Zhang
    • 4
    • 5
  • Zucong Cai
    • 1
    • 6
  • Shenqiang Wang
    • 7
  1. 1.School of Geography SciencesNanjing Normal UniversityNanjingChina
  2. 2.College of ForestryNanjing Forestry UniversityNanjingChina
  3. 3.Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable LandBeijingChina
  4. 4.Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and ApplicationNanjingChina
  5. 5.State Key Laboratory Cultivation Base of Geographical Environment Evolution (Jiangsu Province)NanjingChina
  6. 6.Key Laboratory of Virtual Geographic Environment (Nanjing Normal University), Ministry of EducationNanjingChina
  7. 7.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of SciencesNanjingChina

Personalised recommendations