Biology and Fertility of Soils

, Volume 51, Issue 4, pp 511–516 | Cite as

Manure-associated stimulation of soil-borne methanogenic activity in agricultural soils

  • Adrian Ho
  • Alaa El-Hawwary
  • Sang Yoon Kim
  • Marion Meima-Franke
  • Paul Bodelier
Short Communication

Abstract

The growing human population and scarcity of arable land necessitate agriculture intensification to meet the global food demand. Intensification of agricultural land entails manure input into agrosystems which have been associated to increased methane emission. We investigated the immediate short-term response of methane production and the methanogens after manure amendments in agricultural soils and determined the relevance of the manure-derived methanogenic population in its contribution to soil methane production. We followed methane production in a series of unamended and manure-amended batch incubations: (i) manure and soil, (ii) sterilized manure and soil, and (iii) manure and sterilized soil. Moreover, we determined the methanogenic abundance using a quantitative PCR targeting the mcrA gene. Results show that the soil-borne methanogenic community was significantly stimulated by manure amendment, resulting in increased methane production and mcrA gene abundance; manure-derived methanogenic activity contributed only marginally to overall methane production. Accordingly, our results highlighted the importance of the resident methanogenic community and physiochemical properties of a residue when considering methane mitigation strategies in agricultural soils.

Keywords

Methane production Methanogens Manure-amendment mcrA Agricultural management 

Supplementary material

374_2015_995_Fig3_ESM.jpg (688 kb)
Figure S1

Methane production in un-amended and amended wetland agricultural soil with (A) 10 %, (B) 20 %, and (C) 40 %w/w manure (mean ± s.d; n = 4). (JPEG 687 kb)

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High resolution image (EPS 1194 kb)
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Figure S2

Methane production in un-amended and amended oxic soil with (A) 10 %, (B) 20 %, and (C) 40 %w/w manure (mean ± s.d; n = 4). Note the different scales on the y-axis. (JPEG 869 kb)

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Figure S3

Methane production in soils and gamma-irradiated (sterilized) soils for wetland (A) and oxic (B) agricultural soils, and (C) methane production in incubations containing only (sterilized) manure (mean ± s.d; n = 4). Note the different scales on the x- and y-axis. (JPEG 821 kb)

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Figure S4

Methane production after prolonged incubation (16 d) in sterilized wetland (A) and oxic (B) agricultural soils amended with 10 %w/w manure (mean ± s.d; n = 2) in comparison to other treatments (mean ± s.d; n = 4). Note the different scales on the y-axis. (JPEG 908 kb)

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Figure S5

Correlation of mcrA gene abundance and methane production rate at a significant level (p < 0.005; linear regression). Data was integrated from the un-amended and 10 %w/w manure-amended incubations for both soils. (JPEG 854 kb)

374_2015_995_MOESM5_ESM.eps (1.2 mb)
High resolution image (EPS 1198 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Adrian Ho
    • 1
  • Alaa El-Hawwary
    • 1
  • Sang Yoon Kim
    • 1
  • Marion Meima-Franke
    • 1
  • Paul Bodelier
    • 1
  1. 1.Department of Microbial EcologyNetherlands Institute of Ecology (NIOO-KNAW)WageningenThe Netherlands

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