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Biology and Fertility of Soils

, Volume 50, Issue 5, pp 815–826 | Cite as

Changes in bacterial and archaeal community assemblages along an ombrotrophic peat bog profile

  • Edoardo Puglisi
  • Claudio Zaccone
  • Fabrizio Cappa
  • Pier Sandro Cocconcelli
  • William Shotyk
  • Marco TrevisanEmail author
  • Teodoro M. Miano
Original Paper

Abstract

Peatlands are archives of extreme importance for the assessment of past ecological, environmental and climatic changes. The importance as natural archives is even greater in the case of ombrotrophic peat bogs, where the only inputs are atmospheric in origin. Here we integrated previously published physical and chemical results regarding the solid and liquid phase of peat with a biomolecular microbiological approach to assess the relationships between chemistry and microbial biodiversity along a Swiss bog profile corresponding to approximately 2,000 years of peat formation. The structure of bacterial and archaeal communities was assessed through a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach followed by sequencing of PCR-DGGE bands of interest. Both chemical and microbiological data showed a differentiation of properties along the peat profile, with three major zones identified. Both bacterial and archaeal profiles clustered according to the depth (i.e., age) of samples. Among bacteria, Acidobacteria were recovered primarily in the first layers of the profile, whereas methanogenic archaea were more commonly recovered in the deepest part of the core, corresponding to the occurring anoxic conditions. Finally, a number of sequences had low homologies with known species, especially in bacteria: this points to an almost unknown microbial community adapted to the extreme conditions of peat bogs, which are acidic, rich in dissolved organic C, and predominantly anoxic.

Keywords

Peatlands Ombrotrophic peat bog Bacterial community Archaeal community PCR-DGGE 

Notes

Acknowledgements

This research was conducted within the PRIN 2009 project “Chemical and biomolecular indicators for reconstructing environmental changes in natural archives” (2009NBHPWR), financed by MIUR (Italian Ministry for University and Research). The authors are grateful to Maria Giulia Parisi for her support in the molecular analyses.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Edoardo Puglisi
    • 1
  • Claudio Zaccone
    • 2
    • 3
  • Fabrizio Cappa
    • 1
  • Pier Sandro Cocconcelli
    • 1
  • William Shotyk
    • 3
  • Marco Trevisan
    • 4
    Email author
  • Teodoro M. Miano
    • 5
  1. 1.Istituto di MicrobiologiaUniversità Cattolica del Sacro CuorePiacenzaItaly
  2. 2.Department of the Sciences of Agriculture, Food and EnvironmentUniversity of FoggiaFoggiaItaly
  3. 3.Department of Renewable ResourcesUniversity of AlbertaEdmontonCanada
  4. 4.Istituto di Chimica Agraria ed AmbientaleUniversità Cattolica del Sacro CuorePiacenzaItaly
  5. 5.Department of Soil, Plant and Food SciencesUniversity of Bari “Aldo Moro”BariItaly

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