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Biology and Fertility of Soils

, Volume 49, Issue 8, pp 1109–1121 | Cite as

Impact of 12-year field treatments with organic and inorganic fertilizers on crop productivity and mycorrhizal community structure

  • Saad El Din Hassan
  • Aiguo Liu
  • Shabtai Bittman
  • Thomas A. Forge
  • Derek E. Hunt
  • Mohamed HijriEmail author
  • Marc St-ArnaudEmail author
Original Paper

Abstract

The effect of manure and mineral fertilization on the arbuscular mycorrhizal (AM) fungal community structure of sunflower (Helianthus annuus L.) plants was studied. Soils were collected from a field experiment treated for 12 years with equivalent nitrogen (N) doses of inorganic N, dairy manure slurry, or without N fertilization. Fresh roots of tall fescue (Festuca arundinacea Schreb.) grass collected from the field plots without N fertilization and unfumigated field soils were used as native microbial inoculum sources. Sunflower plants were sown in pots containing these soils, and three different means of manipulating the microbial community were set: unfumigated soil with fresh grass roots, fumigated soil with fresh grass roots, or fumigated soil with sterilized grass roots. Assessing the implications with respect to plant productivity and mycorrhizal community structure was investigated. Twelve AM fungal OTUs were identified from root or soil samples as different taxa of Acaulospora, Claroideoglomus, Funneliformis, Rhizophagus, and uncultured Glomus, using PCR-DGGE and sequencing of an 18S rRNA gene fragment. Sunflower plants grown in manure-fertilized soils had a distinct AMF community structure from plants either fertilized with mineral N or unfertilized, with an abundance of Rhizophagus intraradices-like (B2). The results also showed that AM inoculation increased P and N contents in inorganic N-fertilized or unfertilized plants, but not in manure-fertilized plants.

Keywords

Arbuscular mycorrhizal fungi Biodiversity Manure Nitrogen Fertilization Ribosomal rRNA genes DGGE 

Notes

Acknowledgments

This work was supported by NSERC discovery grants to both MSA and MH and by a fellowship from the Ministry of Higher Education of Egypt to SEH, to which supports are gratefully acknowledged. Funding was also provided by NSERC Metal in the Human Environment (MITHE) grant. We thank Stéphane Daigle for helping with statistical analysis and Cristina Micali for comments and English editing.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Saad El Din Hassan
    • 1
    • 2
  • Aiguo Liu
    • 3
  • Shabtai Bittman
    • 3
  • Thomas A. Forge
    • 3
  • Derek E. Hunt
    • 3
  • Mohamed Hijri
    • 1
    Email author
  • Marc St-Arnaud
    • 1
    Email author
  1. 1.Institut de recherche en biologie végétaleUniversité de Montréal and Jardin botanique de MontréalMontrealCanada
  2. 2.Botany & Microbiology Department, Faculty of ScienceAl-Azhar UniversityNasr CityEgypt
  3. 3.Agriculture and Agri-Food CanadaAgassizCanada

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