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Biology and Fertility of Soils

, Volume 49, Issue 8, pp 1141–1152 | Cite as

Involvement of autoregulation in the interaction between rhizobial nodulation and AM fungal colonization in soybean roots

  • Kazunori SakamotoEmail author
  • Natsuko Ogiwara
  • Tomomitsu Kaji
Original Paper

Abstract

Soybean plants autoregulate to suppress excessive nodulation. It has been revealed recently that the autoregulation of various legumes controls both nodulation and arbuscular mycorrhizal (AM) fungal colonization. We investigated the involvement of autoregulation in the interaction between rhizobial nodulation and AM fungal colonization. We used a wild-type soybean cv. Enrei and its hypernodulating mutant Kanto100, defective in the autoregulation. We included four different treatments: an uninoculated control, inoculation with rhizobium Bradyrhizobium japonicum alone, inoculation with AM fungus Gigaspora rosea alone, and dual inoculation with rhizobium and AM fungus. In both Enrei and Kanto100, AM fungal colonization enhanced the weight and N2 fixation of nodules, suggesting that autoregulation of host plant is not involved in the stimulatory effect of AM fungal colonization on rhizobial nodulation. In plants with the AM fungus alone, the AM fungal colonization of Enrei was comparable to that of Kanto100. In plants with dual inoculation, however, this was significantly (P < 0.05) lower than in Kanto100. To confirm the control of AM fungal colonization by the autoregulation of host plant, a reciprocal grafting experiment was performed between Enrei and Kanto100. In plants with the AM fungus alone, AM fungal colonization was comparable among Enrei (shoot)/Enrei (root), Enrei/Kanto100, Kanto100/Enrei, and Kanto100/Kanto100 grafts. In plants with dual inoculation, however, AM fungal colonization of Enrei/Enrei and Enrei/Kanto100 grafts was significantly (P < 0.05) lower than that of Kanto100/Enrei and Kanto100/Kanto100. These results indicate that rhizobial nodulation suppresses AM fungal colonization, and the autoregulation of host plant, initiated by nodulation, is involved in this phenomenon.

Keywords

Arbuscular mycorrhizal fungi Autoregulation Dual inoculation Rhizobia Soybean Tripartite symbiosis 

Notes

Acknowledgments

We are grateful to Dr. Ryo Yamamoto, NARO Institute of Crop Science, Tsukuba, Japan, for providing soybean seeds. We thank Professor Kazuyuki Inubushi and Dr. Miwa Matsushima-Yashima, Chiba University, for their valuable suggestions. This work was supported by the Japan Society for the Promotion of Science KAKENHI grant numbers 18380047 and 23380042.

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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Kazunori Sakamoto
    • 1
    Email author
  • Natsuko Ogiwara
    • 1
  • Tomomitsu Kaji
    • 1
  1. 1.Department of HorticultureChiba UniversityMatsudoJapan

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