Biology and Fertility of Soils

, Volume 48, Issue 4, pp 463–468 | Cite as

Extraction, characterization, and nematicidal activity of chitin and chitosan derived from shrimp shell wastes

  • Mohamed A. Radwan
  • Samia A. A. Farrag
  • Mahmoud M. Abu-Elamayem
  • Nabila S. Ahmed
Short Communication

Abstract

Chitin has been chemically extracted from the Egyptian shrimp shell waste. The obtained chitin was transformed into the more soluble chitosan. Chitin and chitosan were characterized using Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectrometry. These products were also characterized by their nematicidal potential against the root-knot nematode, Meloidogyne incognita, infecting tomato in a glasshouse. Chitin or chitosan was incorporated into the soil at the rate of 1, 3, 5, and 10 g/kg, and their nematicidal activity was compared with that of the synthetic nematicide oxamyl at the rate of 0.01 g a.i./kg. The effects of the treatments on the growth of tomato were also examined. The obtained results show that chitin and chitosan reduced tomato root galls and J2 of M. incognita in the soil in a dose-dependent manner. Chitosan was more effective in the reduction of galls and J2 in the soil than chitin. However, the efficacy of chitin and chitosan in reducing the number of J2 in the soil was less than that of oxamyl. As compared to the untreated inoculated plants, the length of shoots and roots was significantly (p ≤ 0.05) increased in soil amended with either chitin or chitosan, whereas both treatments did not significantly (p > 0.05) differ with respect to the inoculated control on the effect on the dry weight of shoot and root systems. Furthermore, neither chitin nor chitosan treatments at the tested rates were phytotoxic to tomato plants.

Keywords

Shrimp shell wastes Chitin Chitosan Extraction Characterization Nematicidal activity 

References

  1. Aboud HM, Fattah FA, Al-Heeti AA, Saleh HM (2002) Efficiency of chitosan in inducing systemic acquired resistance against the root-knot nematode (Meloidogyne javanica (Treub) Chitwood) on tomato. Arab J Plant Protect 20:93–98Google Scholar
  2. Badawy MEI, Rabea EI (2011) A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. Inter J Carbohy Chem 2011:1–29CrossRefGoogle Scholar
  3. Bautista-Baños S, Hernández-Lauzardo AN, Velázquez-del Valle MG, Hernández-López M, Ait Barka E, Bosquez-Molina E, Wilson CL (2006) Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Protect 25:108–118CrossRefGoogle Scholar
  4. Bell NI, Watson RN, Sarathchandra SU (2000) Suppression of plant parasitic nematodes in pastoral soils amended with chitin. New Zealand Plant Protect 53:44–47Google Scholar
  5. D’Addabbo T (1995) The nematicidal effect of organic amendments: a review of the literature, 1982–1994. Nematol medit 23:299–305Google Scholar
  6. Ehteshamul-Haque S, Sultana V, Ara J, Qasim R, Ghaffar A (1997) Use of crustacean chitin and plant growth promoting bacteria for the control of Meloidogyne javanica root knot nematode in chickpea. Pak J Nematol 15:89–93Google Scholar
  7. El Hadrami A, Adam LR, El Hadrami I, Daayf F (2010) Chitosan in plant protection. Mar drugs 8:968–987PubMedCrossRefGoogle Scholar
  8. Goodey JB (1963) Laboratory methods for work with plant and soil nematodes. Ministry of Agriculture, Fisheries and Food; Tech. Bull. 2, London, p 44Google Scholar
  9. Hallmann J, Rodriguez-Kabana R, Kloepper JW (1999) Chitin-mediated changes in bacterial communities of the soil rhizosphere and within roots of cotton in relation to nematode control. Soil Biol Biochem 31:551–560CrossRefGoogle Scholar
  10. Hussey RS, Barker KR (1973) A comparison of methods of collecting inocula for Meloidogyne spp. including a new technique. Plant Dis Rep 57:1025–1028Google Scholar
  11. Kalaiarasan P, Lakshmanan P, Rajendran G, Samiyappan R (2006) Chitin and chitinolytic biocontrol agents for the management of root-knot nematode, Meloidogyne arenaria in groundnut (Arachis hypogaea L.) cv. Co3. Indian J Nematol 36:200–205Google Scholar
  12. Khan TA, Saxena SK (1997) Effect of chitin amendments in the control of plant parasitic nematodes associated with cowpea. Pak J Nematol 15:65–69Google Scholar
  13. Kokalis-Burelle N, Martinez-Ochoa N, Rodríguez-Kábana R, Kloepper JW (2002) Development of multi-component transplant mixes for suppression of Meloidogyne incognita on tomato (Lycopersicon esculentum). J Nematol 34:362–369PubMedGoogle Scholar
  14. Korayem AM, Youssef MMA, Mohamed MMM (2008) Effect of chitin and abamectin on Meloidogyne incognita infecting rapeseed. J Plant Protect Res 48:365–370CrossRefGoogle Scholar
  15. Ladner DC, Tchounwou PB, Lawrence GW (2008) Evaluation of the effect of ecologic on root knot nematode, Meloidogyne incognita, and tomato plant, Lycopersicon esculenum. Inter J Environ Res Public Health 5:104–110CrossRefGoogle Scholar
  16. Lopez RJ, de Aymerich VB (2005) Improvement of soil health and quality by reducing plant parasitic nematodes through selected organic amendments. Advan Geoecol 36:421–426Google Scholar
  17. Mian JH, Godoy G, Shelby RA, Rodriguez-Kabana R, Morgan Jones G (1982) Chitin amendments for control of Meloidogyne arenaria in infested soil. Nematropica 12:71–84Google Scholar
  18. Mula W, Hallmann J, Bell D, Kopp-Holtwiesche B, Sikora RA (2001) Effects of natural products on soil organisms and plant health enhancement. Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 66:609–617Google Scholar
  19. No HK, Meyers SP (1989) Crawfish chitosan as a coagulant in recovery of organic compounds from sea food processing streams. J Agric Food Chem 37:580–583CrossRefGoogle Scholar
  20. No HK, Meyers SP, Lee KS (1989) Isolation and characterization of chitin from crawfish shell waste. J Agric Food Chem 37:575–579CrossRefGoogle Scholar
  21. Noling JW, Becker JO (1994) The challenge of research and extension to define and implement alternatives to methyl bromide. J Nematol 26:573–586PubMedGoogle Scholar
  22. Oka Y (2010) Mechanisms of nematode suppression by organic soil amendments—a review. Appl Soil Ecol 44:101–115CrossRefGoogle Scholar
  23. Rich JR, Dunn R, Noling J (2004) Nematicides: past and present uses. In: Chen ZX, Chen SY, Dickson DW (eds) Nematology: advances and perspectives, vol. 2. Nematode management and utilization. CABI Publishing, Oxfordshire, pp 1041–1082Google Scholar
  24. Rodríguez-Kábana R, Morgan-Jones G, Chet I (1987) Biological control of nematodes: soil amendments and microbial antagonists. Plant Soil 100:237–247CrossRefGoogle Scholar
  25. Sadek, S, Rafael R, Shakouri M, Rafomanana G, Ribeiro FL, Clay J (2002) Shrimp aquaculture in Africa and the Middle East: the current reality and trends for the future. Report prepared under the World Bank, NACA, WWF and FAO Consortium Program on Shrimp Farming and the Environment. Work in Progress for Public Discussion. Published by the Consortium, Bangkok, Thailand, pp 1–42Google Scholar
  26. SAS Institute (1998) SAS/STAT user’s guide. Release 6.12, 6th edition. SAS Institute Inc, Carry, p 1028Google Scholar
  27. Shahidi F, Synowiecki J, Naczk M (1992) Utilization of shellfish processing discards. In: Bligh EG (ed) Seafood science and technology. Canadian Institute of Fisheries Technology, Oxford, pp 300–304Google Scholar
  28. Sikora RA, Fernandez E (2005) Nematode parasites of vegetables. In: Luc M, Sikora RA, Bridge J (eds) Plant parasitic nematodes in subtropical and tropical agriculture. CABI Publishing, Wallingford, pp 319–392CrossRefGoogle Scholar
  29. Spiegel Y, Cohn E, Chet I (1986) Use of chitin for controlling plant-parasitic nematodes. I. Direct effects on nematode reproduction and plant performance. Plant Soil 95:87–95CrossRefGoogle Scholar
  30. Spiegel Y, Chet I, Cohn E (1987) Use of chitin for controlling plant-parasitic nematodes. II. Mode of action. Plant Soil 98:337–345CrossRefGoogle Scholar
  31. Tabarant P, Villenave C, Risede J-M, Roger-estrade J, Dorel M (2011) Effects of organic amendements on plant-parasitic nematode populations, root damage, and banana plant growth. Biol Fertil Soils 47:341–348CrossRefGoogle Scholar
  32. Xu Y, Gallert C, Winter J (2008) Chitin purification from shrimp wastes by microbial deproteination and decalcification. Appl Microbiol Biotechnol 79:687PubMedCrossRefGoogle Scholar
  33. Zinov'eva SV, Vasyukova NI, Il'inskaya LI, Varlamov VP, Ozeretskovskaya OL, Sonin MD (1999) Effect of chitosan on interactions in a plant—parasitic nematode system. Doklady Biol Sci 367:400–402Google Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Mohamed A. Radwan
    • 1
  • Samia A. A. Farrag
    • 1
  • Mahmoud M. Abu-Elamayem
    • 1
  • Nabila S. Ahmed
    • 1
  1. 1.Department of Pesticide Chemistry and Technology, Faculty of AgricultureUniversity of AlexandriaAlexandriaEgypt

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