Biology and Fertility of Soils

, Volume 48, Issue 4, pp 453–461 | Cite as

Two-year field study shows little evidence that PPO-transgenic rice affects the structure of soil microbial communities

  • Young Jin Chun
  • Hyo-Jeong Kim
  • Kee Woong Park
  • Soon-Chun Jeong
  • Bumkyu Lee
  • Kyoungwhan Back
  • Hwan Mook KimEmail author
  • Chang-Gi KimEmail author
Short Communication


There is global concern about the environmental consequences associated with transgenic crops. Their effects on the soil ecosystem are of special interest when assessing ecological safety and integrity. Although many efforts have been made to develop crops genetically modified to have resistance to protoporphyrin oxidase (PPO)-inhibiting herbicides, little is known about their influence on soil microbial communities. We conducted a 2-year field study and an analysis via terminal restriction fragment length polymorphism (T-RFLP) to assess the impacts of PPO-transgenic rice on bacterial and fungal communities. In the first year we sampled the rhizosphere and surrounding bulk soil, while in the second year we sampled rhizosphere soil only. No differences were observed in the diversity indices and community composition of microbial communities between transgenic rice and its parental non-transgenic counterpart (cultivar Dongjin). Instead, community variation was strongly dependent on growth stage and year. Therefore, we observed no adverse effects by these crops of modified rice on the microbial community composition in paddy soils.


Protoporphyrin oxidase Rice Soil microbial community T-RFLP Transgenic crop 



This research was supported by grants from the KRIBB Research Initiative Program, the National Research Foundation of Korea (NRF) funded by MEST (No. 20110028162), and the Next-Generation BioGreen 21 Program (No. PJ008008012011), Rural Development Administration, Republic of Korea.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Young Jin Chun
    • 1
  • Hyo-Jeong Kim
    • 2
  • Kee Woong Park
    • 2
  • Soon-Chun Jeong
    • 2
  • Bumkyu Lee
    • 3
  • Kyoungwhan Back
    • 4
  • Hwan Mook Kim
    • 5
    Email author
  • Chang-Gi Kim
    • 2
    Email author
  1. 1.Environmental Appraisal CenterKorea Environment InstituteSeoulRepublic of Korea
  2. 2.Bio-Evaluation CenterKRIBBCheongwonRepublic of Korea
  3. 3.National Academy of Agricultural ScienceRural Development AdministrationSuwonRepublic of Korea
  4. 4.Division of Applied Bioscience and BiotechnologyChonnam National UniversityGwangjuRepublic of Korea
  5. 5.Department of PharmacyGachon University of Medicine and ScienceIncheonRepublic of Korea

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