Biology and Fertility of Soils

, Volume 47, Issue 7, pp 753–766

Spatial variability and biophysicochemical controls on N2O emissions from differently tilled arable soils

  • Mohammad Mofizur Rahman Jahangir
  • Dries Roobroeck
  • Oswald Van Cleemput
  • Pascal Boeckx
Original Paper

Abstract

Nitrous oxide (N2O) emissions, soil microbial community structure, bulk density, total pore volume, total C and N, aggregate mean weight diameter and stability index were determined in arable soils under three different types of tillage: reduced tillage (RT), no tillage (NT) and conventional tillage (CT). Thirty intact soil cores, each in a 25 × 25-m2 grid, were collected to a depth of 10 cm at the seedling stage of winter wheat in February 2008 from Maulde (50°3′ N, 3°43′ W), Belgium. Two additional soil samples adjacent to each soil core were taken to measure the spatial variance in biotic and physicochemical conditions. The microbial community structure was evaluated by means of phospholipid fatty acids analysis. Soil cores were amended with 15 kg NO3-N ha−1, 15 kg NH4+-N ha−1 and 30 kg ha−1 urea-N ha−1 and then brought to 65% water-filled pore space and incubated for 21 days at 15°C, with regular monitoring of N2O emissions. The N2O fluxes showed a log-normal distribution with mean coefficients of variance (CV) of 122%, 78% and 90% in RT, NT and CT, respectively, indicating a high spatial variation. However, this variability of N2O emissions did not show plot scale spatial dependence. The N2O emissions from RT were higher (p < 0.01) than from CT and NT. Multivariate analysis of soil properties showed that PC1 of principal component analysis had highest loadings for aggregate mean weight diameter, total C and fungi/bacteria ratio. Stepwise multiple regression based on soil properties explained 72% (p < 0.01) of the variance of N2O emissions. Spatial distributions of soil properties controlling N2O emissions were different in three different tillages with CV ranked as RT > CT > NT.

Keywords

Spatial heterogeneity N2O emissions Aggregate distribution Denitrification Microbial community structure 

References

  1. Ambus P, Christensen S (1994) Measurement of N2O emission from fertilized grassland: an analysis of spatial variability. J Geophys Res 99:16549–16555CrossRefGoogle Scholar
  2. Azam F, Muller C, Benckiser G, Ottow JCG (2002) Nitrification and denitrification as sources of atmospheric nitrous oxide- role of oxidizable carbon and applied nitrogen. Biol Fertil Soils 35:54–61CrossRefGoogle Scholar
  3. Baggs EM, Stevenson M, Pihlatie M, Regar A, Cook H, Cadisch G (2003) Nitrous oxide emissions following application of residues and fertilizer under zero and conventional tillages. Plant Soil 254:361–370CrossRefGoogle Scholar
  4. Ball BC, Scott A, Parker JP (1999) Field N2O, CO2 and CH4 fluxes in relation to tillage, compaction and soil quality in Scotland. Soil Tillage Res 53:29–39CrossRefGoogle Scholar
  5. Bateman EJ, Baggs EM (2005) Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space. Biol Fertil Soils 41:379–388CrossRefGoogle Scholar
  6. Beauchamp EG, Trevors JT, Paul JW (1989) Carbon sources for bacterial denitrification. Adv Soil Sci 10:113–142Google Scholar
  7. Beheydt D, Boeckx P, Hasan PA, Van Cleemput O (2008) N2O emission from conventional and minimum-tilled soils. Biol Fertil Soils. doi:10.1007/s00374-008-0271-9 Google Scholar
  8. Boeckx P, van Neuland K, van Cleemput O (2011) Short-term effect of tillage intensity on N2O and CO2 emissions. Agron Sustain Dev. doi:10s13593-0001/s13593-011-0001-9 Google Scholar
  9. Boon JJ, De Leeuw JW, Hoek GJ, Vosjan JH (1977) Significance and taxonomic value of iso and anteiso monoenoic fatty acids and branched β-hydroxy acids in Desulfovibrio desulfuricans. J Bacteriol 129:1183–1191PubMedGoogle Scholar
  10. Brettar I, Sanchez-Perez JM, Trémolières M (2002) Nitrate elimination by denitrification in hardwood forest soils of the Upper Rhine floodplain—correlation with redox potential and organic matter. Hydrobiologia 269:11–21CrossRefGoogle Scholar
  11. Calderon FJ, Jackson LE, Scow KM, Rolston DE (2000) Microbial responses to simulated tillage in cultivated and uncultivated soils. Soil Biol Biochem 32:1547–1559CrossRefGoogle Scholar
  12. Calderon FJ, Louise EJ, Kate MS, Dennis ER (2001) Short-term dynamics of nitrogen, microbial activity and phospholipid fatty acids after tillage. Soil Sci Soc Am J 65:118–126CrossRefGoogle Scholar
  13. Castaldi S, Smith KA (1998) Effect of cycloheximide on N2O and NO3- production in a forest and an agricultural soil. Biol Fertil Soils 27:27–34CrossRefGoogle Scholar
  14. Chaves B, De Neve S, Cabrera M, Boeckx P, Van Cleemput O, Hofman G (2005) The effect of mixing organic biological waste materials and high-N crop residues on the short-time N2O emissions from horticultural soil in model experiments. Biol Fertil Soils 41:411–419CrossRefGoogle Scholar
  15. Choudhary MA, Akramkhanov A, Saggar S (2002) Nitrous oxide emissions from a New Zealand cropped soil: tillage effects, spatial and seasonal variability. Agric Ecosyst Environ 93:33–43CrossRefGoogle Scholar
  16. D'Haene K, Vermang J, Cornelis WM, Leroy BLM, Schiettecatte W, De Neve S, Gabriels D, Hofman G (2008) Reduced tillage effects on physical properties of silt loam soils growing root crops. Soil Tillage Res 99:279–290CrossRefGoogle Scholar
  17. Davidson EA (1991) Fluxes of nitrous oxide and nitric oxide from terrestrial ecosystems. In Rogers JE, Whitman WB (Eds.), Microbial production and consumption of greenhouse gases: Methane, Nitrogen oxides and Halomethanes. Am Soc Microbiol, Washington DC, pp. 219–235Google Scholar
  18. De Leenheer L, De Boodt M (1967) Determination of aggregate stability by change in mean weight diameter. Meded Landbouwhogesch Opzoekingsstn Staat Gent 24:290–300Google Scholar
  19. De Vita P, Di Paolo E, Fecondo G, Di Fonzo N, Pisante M (2007) No-tillage and conventional tillage effects on durum wheat yield, grain quality and soil moisture content in southern Italy. Soil Tillage Res 92:69–78CrossRefGoogle Scholar
  20. Del Grosso SJ, Ojima DS, Parton WJ, Mosier AR (2002) Simulated effects of tillage and timing of N fertilizer application on net greenhouse gas fluxes and N losses from agricultural soils in the Midwestern USA. In: Van Ham J., Baede, A.P.M., Guicherit, R., Williams-jacobs, J., (Eds.), Non-CO2 greenhouse gases. Proceedings NCGG 3, Maastricht, Netherlands, 21–23 january 2002, Millpress, Rotterdam, pp. 23–29Google Scholar
  21. Denef K, Roobroeck D, Wadu MCWM, Lootens P, Boeckx P (2009) Microbial community composition and rhizodeposit-carbon assimilation in differently managed temperate grassland soils. Soil Biol Biochem 41:144–153CrossRefGoogle Scholar
  22. Doran JW (1982) Tilling changes soil. Crops Soils 34:10–12Google Scholar
  23. Drury CF, Reynolds WD, Tan CS, Welacky TW, Calder W, McLaughlin NB (2006) Emissions of nitrous oxide and carbon dioxide: Influence of tillage type and nitrogen placement depth. Soil Sci Soc Am J 70: 570-581Google Scholar
  24. Ehhalt D, Prather M, Dentener F, Derwent R, Dlugokencky E, Holland E, Laksen I, Katima J, Kirchhoff V, Matson P, Midgley P, Wang M (2001) Atmospheric chemistry and greenhouse gases. In: Houghton JT et al (eds) Climate change 2001: The scientific basis. Cambridge Univ. Press, Cambridge, pp 239–288Google Scholar
  25. Elliott ET (1986) Aggregate structure and carbon, nitrogen and phosphorus in native and cultivated soils. Soil Sci Soc Am J 50:627–633CrossRefGoogle Scholar
  26. Elmi AA, Madromooto C, Hamel C, Liu A (2003) Denitrification and nitrous to nitrous oxide plus nitrogen ratios in the soil profile under three tillage systems. Biol Fertil Soils 38:340–348CrossRefGoogle Scholar
  27. FAO (2006) World reference base for soil resources. Report 013. FAO, Rome, p 86Google Scholar
  28. Farquharson R, Baldock J (2008) Concepts in modelling N2O emissions from land use. Plant Soil 39:147–167CrossRefGoogle Scholar
  29. Flessa H, Beese F (1995) Effects of sugar beet residues on soil redox potential and N2O emissions. Soil Sci Soc Am J 59:1044–1051CrossRefGoogle Scholar
  30. Folorunso OA, Rolston DE (1984) Spatial variability of field-measured denitrification gas fluxes. Soil Sci Soc Am J 48:1214–1219CrossRefGoogle Scholar
  31. Frey SD, Elliott ET, Paustian K (1999) Bacterial and fungal abundance and biomass in conventional and no-tillage agroecosystems along two climatic gradients. Soil Biol Biochem 31:573–585CrossRefGoogle Scholar
  32. Frostegard A, Baath E (1996) The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fertil Soils 22:59–65CrossRefGoogle Scholar
  33. Granli T, Bockman O (1994) Nitrous oxide from agriculture. Norweg J Agric Sci 12:7–128Google Scholar
  34. Gregorich EG, Rochette P, VandenBygaart AJ, Angers DA (2005) Greenhouse gas contributions of agricultural soils and potential mitigation practices in eastern Canada. Soil Tillage Res 83:53–72CrossRefGoogle Scholar
  35. Grigera MS, Drijber RA, Wienhold BJ (2007) Redistribution of crop residues during row cultivation creates a biologically enhanced environment for soil microorganisms. Soil Tillage Res 94:550–554CrossRefGoogle Scholar
  36. Harris K, Young IM, Gilligan CA, Otten W, Ritz K (2003) Effect of bulk density on the spatial organisation of the fungus Rhizoctonia solani in soil. FEMS Microbiol Ecol 44:45–56PubMedCrossRefGoogle Scholar
  37. IPCC (2007a) Climate change 2007: the physical science basis. Contribution of working group I to the fourth assessment report of the intergovernmental panel on climate change. Cambridge Univ Press, Cambridge, UK and New York, USAGoogle Scholar
  38. IPCC (2007b) Climate change 2007: mitigation. Contribution of working group III to the fourth assessment report of the intergovernmental panel on climate change. Cambridge Univ Press, Cambridge, UK and New York, USAGoogle Scholar
  39. Ishizuka S, Iswandi A, Nakajima Y, Yonemura SL, Sudo S, Tsuruta H, Muriyarso D (2005) The variations of greenhouse gas emissions from soils various land use/cover types in Jambi province, Indonesia. Nutr Cycl Agroecosyst 71:17–32CrossRefGoogle Scholar
  40. Jacinthe PA, Lal R (2006) Spatial variability of soil properties and trace gas fluxes in reclaimed mine land of southeastern Ohio. Geoderma 136:598–608CrossRefGoogle Scholar
  41. Jiangxin Gu, Xuhua Z, Yinghong W, Weixin D, Bo Z, Xin C, Yiyong W, Zhichun Z, Jianguo Z (2007) Regulatory effects of soil properties on background N2O emissions from agricultural soils in China. Plant Soil 295:53–65CrossRefGoogle Scholar
  42. Kaharabata SK, Drury CF, Priesak E, Desjardins RL, McKenny DJ, Tan CS, Reynolds D (2003) Comparing measured and expert-N predicted N2O emissions from conventional till and no till corn treatments. Nutr Cycl Agroecosyst 66:107–118CrossRefGoogle Scholar
  43. Konda R, Ohta S, Ishizuka S, Arai S, Ansori S, Tanaka N, Hardjono A (2008) Spatial structures of N2O, CO2 and CH4 fluxes from Acacia magnum plantation soils during a relatively dry season in Indonesia. Soil Biol Biochem 40:3021–3030CrossRefGoogle Scholar
  44. Larry MZ, Joe MB (2006) Soil aggregation, aggregate carbon and nitrogen, and moisture retention induced by conservation tillage. Soil Sci Soc Am J 71:793–802Google Scholar
  45. Laughlin RJ, Stevens RJ (2002) Evidence for fungal dominance of denitrification and co-denitrification in a grassland soil. Soil Sci Soc Am J 66:1540–1548CrossRefGoogle Scholar
  46. Laverman AM, Zoomer HR, Engelbrecht D, Berg MP, van Straalen NM, van Verseveld HW, Verhoef HW (2000) Soil layer specific variability in net nitrification and denitrification in an acid coniferous forest. Biol Fertil Soils 32:427–434CrossRefGoogle Scholar
  47. Lemke RL, Izaurralde RC, Malhi SS, Arshad MA, Nyborg M (1999) Nitrous oxide emissions from agricultural soils of the Boreal and Parkland regions of Alberta. Soil Sci Soc Am J 62:1096–1102CrossRefGoogle Scholar
  48. Lemke RL, Izaurralde RC, Nyborg M, Solberg ED (2004) Seasonal nitrous oxide emissions from agricultural soils in the Parkland region of the Canadian Prairie. Available online:http://paridss.usask.ca/factbook/soilcrop/cesar1.html; consulted on 10.12.2010
  49. Liu XJ, Mosier AR, Halvorson AD, Reule CA, Zhang FS (2007) Dinitrogen and N2O emissions in arable soils: Effects of tillage, N source and soil moisture. Soil Biol Biochem 39:2362–2370CrossRefGoogle Scholar
  50. MacKenzie AF, Fan MF, Cadrin F (1997) Nitrous oxide emission as affected tillage, corn-soybean-alfalfa rotations and nitrogen fertilization. Canad J Soil Sci 77:145–152CrossRefGoogle Scholar
  51. Malhi SS, Lemke RL, Wang Z, Farrel R, Chhabra BS (2006) Tillage, nitrogen and crop residue effects on crop yield and nutrient uptake, soil quality and greenhouse gas emissions. Soil Tillage Res 90:171–183CrossRefGoogle Scholar
  52. Mathieu O, Leveque J, Heault C, Milloux MJ, Bizouard F, Andreux F (2006) Emissions and spatial variability of N2O, N2 and nitrous oxide mole fraction at the field scale, revealed with 15 N isotopic techniques. Soil Biol Biochem 38:941–951CrossRefGoogle Scholar
  53. Mikha MM, Rice CW (2004) Tillage and manure effects on soil and aggregate associated carbon and nitrogen. Soil Sci Soc Am J 68:809–816CrossRefGoogle Scholar
  54. Mosier AR, Halvorson AD, Reule CA, Liu XJ (2006) Net global warming potential and greenhouse gas intensity in irrigated cropping systems in north eastern Colorado. J Environ Qual 35:1584–1598PubMedCrossRefGoogle Scholar
  55. Otten W, Gilligan CA, Watts CW, Dexter AR, Hall D (1999) Continuity of air-filled pores and invasion thresholds of soil borne fungal plant pathogen Rhizoctonia Solani. Soil Biol Biochem 31:1803–1810CrossRefGoogle Scholar
  56. Parkin TB (1987) Soil microsites as a source of denitrification variability. Soil Sci Soc Am J 51:1194–1199CrossRefGoogle Scholar
  57. Peterson SO, Klug MJ (1994) Effects of sieving, storage and incubation temperature on the phospholipid fatty acid of a soil microbial community. Appl Environ Microbiol 60:2421–2430Google Scholar
  58. Philippot L, Hallin S (2005) Finding the missing link between diversity and activity using denitrifying bacteria as a model functional community. Curr Opin Microbiol 8:234–239PubMedCrossRefGoogle Scholar
  59. Prather M, Ehhalt D, Dentener F, Derwent R, Dlugokencky E, Holland E, Isaksen I, Katima J, Kirchoff C, Matson P, Midgley P, Wang M (2001) Atmospheric chemistry and greenhouse gases. In: Houghton JT, Ding W, Griggs DJ, Noguer M, van der Linden PJ, Dai X, Maskell K, Johnson CA (eds) Climate change 2001: the scientific basis. Contribution of Working Group I to the Third Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, UK, pp 240–287Google Scholar
  60. Rahman MS, Okubo A, Sugiyama S, Mayland HF (2008) Physical, chemical and microbiological properties of an Andisol as related to land use and tillage practice. Soil Tillage Res 101:10–19CrossRefGoogle Scholar
  61. Rochette P (2008) No-till only increases N2O emissions in poorly-aerated soils. Soil Tillage Res 101:97–100CrossRefGoogle Scholar
  62. Rochette P, Angers DA, Chantigny MH, Bertrand N (2008) Nitrous oxide emissions respond differently to No-till in a loam and a heavy clay soil. Soil Sci Soc Am J 72:1363–1369CrossRefGoogle Scholar
  63. Ruser R, Flessa H, Russow R, Schmidt G, Buegger F, Munch JC (2006) Emissions of N2O, N2 and CO2 from soil fertilized with nitrate: effect of compaction, soil moisture and rewetting. Soil Biol Biochem 6:1–16Google Scholar
  64. SAS (2003) SAS Institute Inc., Cary, NC, USAGoogle Scholar
  65. Scholefield D, Hawkins JMB, Jackson SM (1997) Use of flowing helium atmosphere incubation technique to measure the effects of denitrification controls applied to intact cores of a clay soil. Soil Biol Biochem 29:1337–1344CrossRefGoogle Scholar
  66. Sexstone AJ, Revsbech NP, Parkin TB, Tiedje JM (1985) Direct measurement of oxygen profiles and denitrification rates in soil aggregates. Soil Sci Soc Am J 49:645–651CrossRefGoogle Scholar
  67. Shoun H, Kim D, Uchiyama H, Sugiyama J (1992) Denitrification by fungi. FEMS Microbiol Lett 94:277–282CrossRefGoogle Scholar
  68. Simek M, Brucek P, Hynst J, Uhlirova E, Petersen SO (2006) Effects of excretal returns and soil compaction of nitrous oxide emissions from a cattle over-wintering area. Agric Ecosyst Environ 112:186–191CrossRefGoogle Scholar
  69. Sitaula BK, Hansen S, Sitaula JIB, Bakken LR (2000) Effects of soil compaction on N2O emission in agricultural soil. Chemosphere Glob Chang Sci 2:367–371CrossRefGoogle Scholar
  70. Skinner MF, Bowen GD (1974) The penetration of soil by mycelial strands of ectomycorrhizal fungi. Soil Biol Biochem 6:57–58CrossRefGoogle Scholar
  71. Smith KA (1990) Anaerobic zones and denitrification in soil: modelling and measurement. In: Revsbech S (ed) Denitrification in Soil and Sediment. Plenum Press, New York, pp 229–244Google Scholar
  72. Stahl PD, Klug MJ (1996) Characterization and differentiation of filamentous fungi based on fatty acid composition. Appl Environ Microbiol 62:4136–4146PubMedGoogle Scholar
  73. Stoyan H, De-Polli H, Bohm S, Robertson GP, Paul EA (2000) Spatial heterogeneity of soil respiration and related properties at the plant scale. Plant Soil 222:203–214CrossRefGoogle Scholar
  74. Swerts M, Merckx R, Vlassak K (1996) Influence if C availability on the production of NO, N2O, N2 and CO2 by soil cores during anaerobic incubation. Plant Soil 181:145–151CrossRefGoogle Scholar
  75. The R Foundation for Statistical Computing (2006) version 2.7.2. http://www.r-project.org/foundation. Accessed 12 Dec 2010
  76. Uchida Y, Clough TJ, Kelliher FM, Sherlock RR (2008) Effects of aggregate size, soil compaction, and bovine urine on N2O emissions from a pasture soil. Soil Biol Biochem 40:924–931CrossRefGoogle Scholar
  77. Van den Heuvel RN, Hefting MM, Tan NCG, Jetten MSM, Verhoeven JTA (2009) N2O emission hotspots at different spatial scales and governing factors for small scale hotspots. Sci Total Environ 4007:2325–2332Google Scholar
  78. Venterea RT, Burger M, Spokas KA (2005) Nitrogen oxide and methane emissions under varying tillages and fertilizer management. J Environ Qual 34:1467–1477PubMedCrossRefGoogle Scholar
  79. Vian JF, Peigne J, Chaussod R, Roger-Estrade J (2009) Effects of four tillage systems on soil structure and soil microbial biomass in organic farming. Soil Use Manage 25:1–10CrossRefGoogle Scholar
  80. Wang L, Sun X, Cai Y, Xie H, Zhang X (2008) Relationships of soil physical and microbial properties with nitrous oxide emission affected by freeze-thaw even. Front Agric China 2:290–295CrossRefGoogle Scholar
  81. Webster EA, Hopkins DW (1996) Contributions from different microbial process to N2O emission from soil under different moisture regimes. Biol Fertil Soils 22:331–335CrossRefGoogle Scholar
  82. Weitz AM, Linder E, Frolking S, Crill PM, Keller M (2001) N2O emissions from humid tropical agricultural soils: effects of soil moisture, texture and nitrogen availability. Soil Biol Biochem 33:1077–1093CrossRefGoogle Scholar
  83. Xiong Z, Xie Y, Xing G, Zhu Z, Butenhoff C (2006) Measurements of nitrous oxide emissions from vegetables production in China. Atmos Environ 40:2225–2234CrossRefGoogle Scholar
  84. Yanai J, Sawamoto T, Oe T, Kusa K, Yamakawa K, Sakamoto K, Naganawa T, Inubushi K, Hatano R, Kosaki T (2003) Spatial variability of nitrous oxide and their soil-related determining factors in an agricultural field. J Environ Qual 32:1965–1977PubMedCrossRefGoogle Scholar
  85. Yates TT, Si BC, Farrel RE, Pennock DJ (2006) Probability distribution and spatial dependence of nitrous oxide emission: temporal change in hummocky terrain. Soil Sci Soc Am J 70:753–762CrossRefGoogle Scholar
  86. Young IM, Ritz K (2000) Tillage, habitat space and function of soil microbes. Soil Tillage Res 53:201–213CrossRefGoogle Scholar
  87. Zelles L (1997) Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review. Biol Fertil Soils 29:111–129CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Mohammad Mofizur Rahman Jahangir
    • 1
    • 2
  • Dries Roobroeck
    • 2
  • Oswald Van Cleemput
    • 2
  • Pascal Boeckx
    • 2
  1. 1.Department of Civil, Structural & Environmental Engineering, Museum BuildingTrinity College DublinDublinIreland
  2. 2.Laboratory of Applied Physical Chemistry (ISOFYS)Ghent UniversityGhentBelgium

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