Biology and Fertility of Soils

, Volume 46, Issue 2, pp 169–174

Co-inoculation of the endophytic fungus Piriformospora indica with the phosphate-solubilising bacterium Pseudomonas striata affects population dynamics and plant growth in chickpea

  • Kamlesh K. Meena
  • Sukumar Mesapogu
  • Manish Kumar
  • Mahesh S. Yandigeri
  • Geeta Singh
  • Anil K. Saxena
Original Paper

Abstract

In this study, inoculation of the chickpea (Cicer arietinum) with the novel symbiotic fungus (Piriformospora indica) was analysed in combination with the Tn5-lacZ-tagged phosphate-solubilising bacterium Pseudomonas striata. This study aims to evaluate whether the co-inoculation of these two species would enhance the population buildup of P. striata in the rhizosphere, P uptake, growth and yield of chickpea. Single inoculation of P. indica and P. striata has a negative effect on plant growth and yield of chickpea. Data showed that the combination of the two microorganisms had a synergistic effect on population buildup of P. striata and plant dry biomass with respect to their single inoculation. However, the P uptake was not significantly influenced by single or combined inoculation of two species. At 20 days after sowing, the influence of combined inoculation on the population of P. striata was positive; at 60 days after sowing, it was neutral as the populations in treatments with single and combined inoculation were at par; and at harvest, it was negative The population of P. striata was higher at flowering stage as compared to 20 days after sowing and at harvest.

Keywords

Pseudomonas striata Piriformospora indica Chickpea Rhizosphere Population dynamics 

References

  1. Adesemoye AO, Torbert HA, Kloepper JW (2008) Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Can J Microbiol 54:876–886CrossRefPubMedGoogle Scholar
  2. Barea JM, Azcon R, Azcon-Aguilar C (2004) Mycorrhizal fungi and PGPR. In: Kamp RM, Calvete JJ, Choli-Papadopoulou T (eds) Principles and practice, methods in proteome and protein analysis. Springer, BerlinGoogle Scholar
  3. Bashan Y, de-Bashan LE (2005) Bacteria/plant growth-promotion. In: Hillel D (ed) Encyclopedia of soils in the environment, vol 1. Elsevier, OxfordGoogle Scholar
  4. Bucio JL, Campos-Cuevas JC, Hernandez-Calderon E, Valasquez-Bacerra C, Farias-Rodriguez R, Macias-Rodriguez LI, Valencia-Cantero E (2007) Bacillus megaterium rhizobacteria promote growth and alter root system architecture through an auxin and ethylene-independent signaling mechanism in Arabidopsis thaliana. Mol Plant Microbe Interactions 20:207–217CrossRefGoogle Scholar
  5. Deshmukh S, Huckelhoven R, Schafer P, Imani J, Sharma M, Weiss M, Waller F, Kogel K (2006) The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley. Proc Nat Acad Sci 103:18450–18457CrossRefPubMedGoogle Scholar
  6. Franke IH, Fegan M, Hayward C, Leonard G, Sly LI (2000) Molecular detection of Gluconoacetobacter sacchari associated with the pink sugarcane mealy bug Saccharicoccus sacchari (Cockerell) and the sugarcane leaf sheath microenvironment by FISH and PCR. FEMS Microbiol Ecol 31:61–71CrossRefPubMedGoogle Scholar
  7. Hardoim PR, Overbeek V, Leo S, Elsas DJV (2008) Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol 16:463–471CrossRefPubMedGoogle Scholar
  8. Hoflich G, Wiehe W, Kuhn G (1994) Plant growth stimulation with symbiotic and associative rhizoshpere microorganisms. Experientia 50:897–905CrossRefGoogle Scholar
  9. Isopi R, Fabbri P, Degallo M, Puppi G (1995) Dual inoculation of Sorghum bicolor (L.) Moench sp. bicolor with vesicular–arbuscular mycorrhizas and Acetobacter diazotrophicus. Symbiosis 18:43–55Google Scholar
  10. Jackson ML (1973) Soil chemical analysis. Prentice Hall, New Delhi, p 183Google Scholar
  11. Kaefer E (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Advances Genet 19:33–131CrossRefGoogle Scholar
  12. Kloepper JW, Hume DJ, Schner FM, Singleton C, Tipping B, Laliberte M, Frauley K, Kutchaw T, Simonson C, Lifshitz R, Zaleska I, Lee L (1988) Plant growth promoting rhizobacteria on canola. Plant Diseases 72:42–46CrossRefGoogle Scholar
  13. Kohler J, Caravaca F, Carrasco L, Roldan A (2007) Interaction between a PGPR, an AM fungus and a phosphate solubilizing fungus in the rhizosphere of Lactuca sativa. Appl Soil Ecol 35:480–487CrossRefGoogle Scholar
  14. Linderman RG (1992) Vesicular-arbuscular mycorrhizae and soil microbial interactions. In: Bethlenfalvay GJ, Linderman RG (eds) Agriculture. American Society of Agronomy, Madison, pp 45–70Google Scholar
  15. Marschner P, Timonen S (2004) Interaction between plant species, mycorrhizal colonization and light intensity on the bacterial community composition in the rhizosphere. Appl Soil Ecol 28:23–36CrossRefGoogle Scholar
  16. Marschner P, Crowley DE, Lieberei R (2001) Arbuscular mycorrhizal infection changes the bacterial 16S rDNA community composition in the rhizosphere of maize. Mycorrhiza 11:297–302CrossRefGoogle Scholar
  17. McInroy JA, Kloepper JW (1995) Population dynamics of endophytic bacteria in field grown sweet corn and cotton. Can J Microbiol 41:895–901CrossRefGoogle Scholar
  18. Diby P, Sarma YR, Srinivasan V, Anandraj M (2005) Pseudomonas fluorescens mediated vigour in black pepper (Piper nigrum L.) under green house cultivation. Ann Microbiol 55:171–174Google Scholar
  19. Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologia 17:362–370Google Scholar
  20. Rodriguez H, Fraga R, Gonzalez T, Bashan Y (2006) Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant Soil 287:15–21CrossRefGoogle Scholar
  21. Roesti D, Gaur R, Johri BN, Imfeld G, Sharma S, Kawaljeet K, Aragno M (2006) Plant growth stage, fertilizer management and bio-inoculations of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biol Biochem 38:1111–1120CrossRefGoogle Scholar
  22. Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
  23. Simon R, Quandt J, Klipp W (1989) New derivatives of transposon Tn5 suitable for mobilization of replicon, generation of operon fusions and induction of genes in Gram-negative bacteria. Gene 80:161–169CrossRefPubMedGoogle Scholar
  24. Singh M, Klingmuller W (1986) Transposon mutagenesis in Azospirillum brasilense, isolation of auxotrophic and Nif mutants and molecular cloning of the mutagenized nif DNA. Mol Gen Genet 196:413–420Google Scholar
  25. Sirrenberg ACG, Stephanie G, Czempinski N, Karlovsky ARP, Santos P, Katharina IF (2007) Piriformospora indica affects plant growth by auxin production. Physiologia Plantarum 131:581–589CrossRefPubMedGoogle Scholar
  26. Smith RS (1995) Inoculant formulations and application to meet changing needs. In: Tikhonovich IA, Provorov NA, Romonov VI, Newton WA (eds) Nitrogen fixation: fundamentals and applications. Kluwer, Dordrecht, pp 653–657Google Scholar
  27. Varma A, Verma S, Sudha N, Buetehorn B, Franken P (1999) Piriformospora indica, acultivable plant growth promoting root endophyte. Appl Environ Microbiol 65:2741–2744PubMedGoogle Scholar
  28. Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586CrossRefGoogle Scholar
  29. Weller F, Achatz B, Baltruschat H, Fodor J, Becker K, Fischer M, Heier T, Huckelhoven R, Neumann C, Wettstein DV, Franken P, Kogel K (2005) The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance and higher yield. Proc Nat Acad Sci 102:13386–13391CrossRefGoogle Scholar
  30. Zaidi A, Khan MS, Amil M (2003) Interactions effect of rhizotrophic microorganisms on yie;ld and nutrient uptake of chickpea (Cicer arietinum L.). Eur J Agron 19:15–21CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Kamlesh K. Meena
    • 1
  • Sukumar Mesapogu
    • 1
  • Manish Kumar
    • 1
  • Mahesh S. Yandigeri
    • 1
  • Geeta Singh
    • 2
  • Anil K. Saxena
    • 2
  1. 1.National Bureau of Agriculturally Important MicroorganismsMau Nath BhanjanIndia
  2. 2.Division of MicrobiologyIndian Agricultural Research InstituteNew DelhiIndia

Personalised recommendations