Biology and Fertility of Soils

, Volume 46, Issue 1, pp 45–55 | Cite as

Pseudomonas sp. strain AKM-P6 enhances tolerance of sorghum seedlings to elevated temperatures

  • Sk. Z. Ali
  • V. Sandhya
  • Minakshi Grover
  • N. Kishore
  • L. Venkateswar Rao
  • B. Venkateswarlu
Original Paper

Abstract

A thermotolerant strain AKM-P6 of Pseudomonas sp. possessing plant growth-promoting properties was isolated from rhizosphere of pigeon pea grown under semiarid conditions in India. The effect of inoculation with AKM-P6 on survival and growth of sorghum seedlings at elevated temperatures (ET) was investigated under sterile and nonsterile soil conditions. Inoculation with strain AKM-P6 helped sorghum (var CSV-15) seedlings to survive and to grow at elevated temperatures (47–50°C day/30–33°C night) up to 15 days while uninoculated plants died by the fifth day of exposure to elevated temperature. Under sterile and nonsterile conditions, significantly higher root and shoot biomass were recorded in inoculated seedlings as compared to uninoculated control at ET, but this difference was nonsignificant at ambient temperature. Inoculation induced the biosynthesis of high-molecular weight proteins in leaves under elevated temperature, reduced membrane injury, and improved the levels of cellular metabolites like proline, chlorophyll, sugars, amino acids, and proteins. Scanning electron microscopy studies confirmed the colonization and establishment of the organism on the root surface. The 16SrDNA sequence of the strain AMK-P6 showed 97% homology with that of Pseudomonas aeruginosa in the existing database. The results indicate that Pseudomonas sp. strain AKM-P6 can enhance tolerance of sorghum seedlings to elevated temperatures by inducing physiological and biochemical changes in the plant.

Keywords

Pseudomonas Thermotolerance Abiotic stress Sorghum PGPR 

References

  1. Abrol YP, Ingram KT (1996) Effect of higher day and night temperatures on growth and yields of some crop plants. In: Bazzaz F, Sombroek W (eds) Global climate change and agricultural production. John Wiley & Sons and FAO, United Nations, Rome, Italy, pp 123–140Google Scholar
  2. Ait Barka E, Nowak J, Clement C (2006) Enhancement of chilling resistance of inoculated grapevine plantlets with a plant growth promoting rhizobacterium; Burkholderia phytofirmans strain PsJn. Appl Environ Microbiol 72:7246–7252CrossRefPubMedGoogle Scholar
  3. Baker JT, Allen LH Jr (1993) Contrasting crop species response to CO2 and temperature: rice, soybean and citrus. Vegetatio 104(105):239–260CrossRefGoogle Scholar
  4. Bakker AW, Schipper B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas sp. Mediated plant growth stimulation. Soil Boil Biochem 19:451–457CrossRefGoogle Scholar
  5. Bano A, Fatima M (2009) Salt tolerance in Zea mays (L) following inoculation with Rhizobium and Pseudomonas. Biol Fertil Soils 45:405–413CrossRefGoogle Scholar
  6. Barnes JD, Balaguer L, Maurigue E, Elvira S, Davison AW (1992) A reappraisal of the use of DMSO for the extraction and determination of chlorophyll “a” and “b” in lichens and higher plants. Environ Exp Bot 32:87–99CrossRefGoogle Scholar
  7. Bates LS, Waldren RD, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  8. Bozzola JJ, Russell LD (1999) Electron microscopy principles and techniques for biologists 2nd ed. Boston: Jones and Bartlett. pp 19–24, 54–55 and 63–67Google Scholar
  9. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–258CrossRefPubMedGoogle Scholar
  10. Burton K (1956) A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323PubMedGoogle Scholar
  11. Chen WP, Kuo TT (1993) A Simple and rapid method for the preparation of Gram-negative bacterial genomic DNA. Nucl Acids Res 21:2260CrossRefPubMedGoogle Scholar
  12. Chen Z, Cuin TA, Zhou M, Twomey A, Naidu BP, Shabala S (2007) Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance. J Exp Bot 58:4245–4255CrossRefPubMedGoogle Scholar
  13. Dell’ Amico E, Cavalca L, Andreoni V (2008) Improvement of Brassica napus growth under cadmium stress by cadmium-resistance rhizobacteria. Soil Biol Biochem 40:74–84CrossRefGoogle Scholar
  14. Dey R, Pal KK, Bhatt DM, Chauhan SM (2004) Growth promotion and yield enhancement of pea nut (Arachis hypogaea l) by application of plant growth promoting rhizobacteria. Microbiol Res 159:371–394CrossRefPubMedGoogle Scholar
  15. Drigo B, Kowalchuck GA, Van Veen JA (2008) Climatic change goes underground: effects of elevated atmospheric CO2 on microbial community structure and activities in the rhizosphere. Biol Fertil Soils 44:667–679CrossRefGoogle Scholar
  16. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:30–356CrossRefGoogle Scholar
  17. Fett WF, Osman SF, Dunn MF (1989) Characterization of exopolysaccharides produced by plant associated Fluorescent pseudomonads. Appl Environ Microbiol 52:579–583Google Scholar
  18. Fischer G, Shah M, Harrij van Velthuizen (2002) Climate change and agricultural vulnerability. A special report prepared by the inter institute for applied system analysis under UNICA No.113, World Summit on Sustainable Development, JohannesburgGoogle Scholar
  19. Gordon AS, Weber RP (1951) Colorimetric estimation of indole acetic acid. Plant Physiol 26:192–195CrossRefPubMedGoogle Scholar
  20. Gouesbet G, Jan G, Boyaval P (2002) Two dimensional electrophoresis study of Lactobacillus delbrueckii subsp. bulgaricus thermotlerance. Appl Environ Microbiol 68:1055–1063CrossRefPubMedGoogle Scholar
  21. Han HS, Lee KD (2005) Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Res J Agric Biol Sci 1:210–215Google Scholar
  22. Hartel PG, Alexandre M (1986) Role of extracellular polysaccharide production and clays in the desiccation tolerance of cowpea Bradyrhizobia. Soil Sci Soc Am J 50:1193–1198Google Scholar
  23. Holbrook AA, Edge WJW, Baily F (1961) Spectrophotometric method for determination of gibberellic acid. Adv Chem Ser 28:159–167CrossRefGoogle Scholar
  24. Holt JG, Krieg NR, Sneath PHA, Stalely JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, BaltimoreGoogle Scholar
  25. Hong Y, Glick BR, Pasternak JJ (1991) Plant-microbial interaction under gnotobiotic condition: a scanning microscope study. Curr Microbiol 23:111–114CrossRefGoogle Scholar
  26. Howarth CJ (1991) Molecular response of plants to an increased incidence of heat shock. Plant Cell Environ 14:831–841CrossRefGoogle Scholar
  27. Ibrahim AMH, Quick JS (2001) Genetic control of high temperature tolerance in wheat as measured by membrane thermal stability. Crop Sci 41:1405–1407CrossRefGoogle Scholar
  28. Lalucat J, Bennasar A, Bosch R, García-Valdés E, Palleroni NJ (2006) Biology of Pseudomonas stutzeri. Microbiol Mol Biol Rev 70:510–547CrossRefPubMedGoogle Scholar
  29. Lindquist S (1986) The heat shock response. Ann Rev Biochem 55:1151–1191CrossRefPubMedGoogle Scholar
  30. Lowery OH, Rosebrough NJ, Farr AL, Randall J (1951) Protein measurement with Folin Phenol reagent. J Biol Chem 193:265–275Google Scholar
  31. Marquez LM, Redman RS, Rodrigues RJ, Roosinck J (2007) A virus in a fungus in a plant: three way symbiosis required for thermal tolerance. Science 315:513–515CrossRefPubMedGoogle Scholar
  32. McLellan CA, Turbyville TJ, Wijeratne EMK, Kerschen EV, Queitsch C, Whitesell L, Gunatilaka AAL (2007) A rhizosphere fungus enhances Arabidopsis thermotolerance through production of an HSP90 inhibitor. Plant Physiol 145:174–182CrossRefPubMedGoogle Scholar
  33. Mehta S, Nautiyal CS (2001) An efficient method for qualitative screening of phosphate solubilizing bacteria. Curr Microbiol 43:51–56CrossRefPubMedGoogle Scholar
  34. Redman RS, Sheehan KB, Stout RG, Rodriguez RJ, Henson JM (2002) Thermotolarance generated by plant/fungal symbiosis. Science 298:1581CrossRefPubMedGoogle Scholar
  35. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor laboratory, Cold Spring, Harbor, NewYorkGoogle Scholar
  36. Samra JS, Sing G (2004) Heat wave of March 2004: impact on agriculture natural resource management division. Indian council of Agricultural Research, New Delhi, p 32Google Scholar
  37. Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of Siderophore. Anal Biochem 160:47–56CrossRefPubMedGoogle Scholar
  38. Sinha SK, Singh GB, Rai M (1998) Decline in crop productivity in Haryana and Punjab: myth or reality? Indian council of Agricultural Research, New Delhi, p 89Google Scholar
  39. Srivastava S, Yadav A, Seem K, Mishra S, Choudhary V, Nautiyal CS (2008) Effect of high temperature on Pseudomonas putida NBRI0987 biofilm formation and expression of stress sigma factor RpoS. Curr Microbiol 56:453–457CrossRefPubMedGoogle Scholar
  40. Timmusk S, Wagner EGH (1999) The plant growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thalianan gene expression: a possible connection between biotic and abiotic stress responses. Mol Plant-Microb Interact 12:951–959CrossRefGoogle Scholar
  41. Vanaja M, Ramakrishna YS, Rao GGSN, Rao KV, Subba rao VM (2007) Climate change and dry land agriculture. Dryland Ecosystems: Indian Perspective. Central Arid zone Research Institute (CAZRI) and Arid Forest Research Institute (AFRI) Jodhpur, India, pp 23–24Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sk. Z. Ali
    • 2
  • V. Sandhya
    • 2
  • Minakshi Grover
    • 2
  • N. Kishore
    • 2
  • L. Venkateswar Rao
    • 1
  • B. Venkateswarlu
    • 2
  1. 1.Department of MicrobiologyOsmania UniversityHyderabadIndia
  2. 2.Central Research Institute for Dryland AgricultureHyderabadIndia

Personalised recommendations